##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632805.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	247325
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.09884564843829	33.0	33.0	33.0	27.0	33.0
2	30.76234509248964	33.0	33.0	33.0	27.0	33.0
3	31.225759628019812	33.0	33.0	33.0	27.0	33.0
4	31.39258061255433	33.0	33.0	33.0	27.0	33.0
5	31.513441827554836	33.0	33.0	33.0	27.0	33.0
6	34.19158192661478	37.0	33.0	37.0	27.0	37.0
7	34.38645102597796	37.0	37.0	37.0	27.0	37.0
8	34.56652582634186	37.0	37.0	37.0	27.0	37.0
9	34.64980895582735	37.0	37.0	37.0	27.0	37.0
10	34.73178611139189	37.0	37.0	37.0	27.0	37.0
11	34.71439199433943	37.0	37.0	37.0	27.0	37.0
12	34.690666127564945	37.0	37.0	37.0	27.0	37.0
13	34.674125138987165	37.0	37.0	37.0	27.0	37.0
14	34.660511472758515	37.0	37.0	37.0	27.0	37.0
15	34.674869099363185	37.0	37.0	37.0	27.0	37.0
16	34.69374709390478	37.0	37.0	37.0	27.0	37.0
17	34.66546042656424	37.0	37.0	37.0	27.0	37.0
18	34.68342060042454	37.0	37.0	37.0	27.0	37.0
19	34.682947538663704	37.0	37.0	37.0	27.0	37.0
20	34.67786111391893	37.0	37.0	37.0	27.0	37.0
21	34.66307490144547	37.0	37.0	37.0	27.0	37.0
22	34.67438795107652	37.0	37.0	37.0	27.0	37.0
23	34.631808349337916	37.0	37.0	37.0	27.0	37.0
24	34.646392398665725	37.0	37.0	37.0	27.0	37.0
25	34.62865056100273	37.0	37.0	37.0	27.0	37.0
26	34.57920549883756	37.0	37.0	37.0	27.0	37.0
27	34.503175983018295	37.0	37.0	37.0	27.0	37.0
28	34.457208126958456	37.0	37.0	37.0	27.0	37.0
29	34.50032952592743	37.0	37.0	37.0	27.0	37.0
30	34.445397755989084	37.0	37.0	37.0	27.0	37.0
31	34.44441524310118	37.0	37.0	37.0	27.0	37.0
32	34.460701506115434	37.0	37.0	37.0	27.0	37.0
33	34.43696350955221	37.0	37.0	37.0	27.0	37.0
34	34.34204791266552	37.0	37.0	37.0	27.0	37.0
35	34.3174203982614	37.0	37.0	37.0	27.0	37.0
36	34.293561103810774	37.0	37.0	37.0	27.0	37.0
37	34.31325179419792	37.0	37.0	37.0	27.0	37.0
38	34.15855251187708	37.0	37.0	37.0	27.0	37.0
39	34.2107146467199	37.0	37.0	37.0	27.0	37.0
40	34.230902658445366	37.0	37.0	37.0	27.0	37.0
41	34.24613362983928	37.0	37.0	37.0	27.0	37.0
42	34.14123117355706	37.0	37.0	37.0	27.0	37.0
43	33.88011321136157	37.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	14.0
16	15.0
17	16.0
18	14.0
19	22.0
20	42.0
21	122.0
22	316.0
23	699.0
24	1365.0
25	2156.0
26	3371.0
27	4776.0
28	6391.0
29	8341.0
30	10273.0
31	12472.0
32	15342.0
33	19534.0
34	26568.0
35	44949.0
36	90527.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.331042151015865	20.984938845648436	13.779844334377845	23.904174668957847
2	15.824522389568383	24.53977559890832	37.29586576367128	22.339836247852016
3	18.44374810472051	27.756999898918426	30.858991205903163	22.9402607904579
4	12.534115030829879	18.340240574143333	40.247852016577376	28.877792378449406
5	14.022844435459417	37.203679369250985	35.09026584453654	13.683210350753058
6	28.283837056504602	41.29263115334075	17.259072071161427	13.164459718993227
7	25.341554634590114	33.25341150308299	23.59526938239159	17.809764479935307
8	24.39057919741231	35.48124936824017	21.171737592236937	18.956433842110584
9	26.160315374507228	15.014252501768926	20.43788537349641	38.387546750227436
10	14.486202365308804	28.902456282219752	34.394824623471145	22.216516729000304
11	33.6747194986354	24.184372788840594	24.39098352370363	17.74992418882038
12	22.630951177600323	26.53795613059739	30.967754978267465	19.863337713534822
13	28.889517840897604	22.634994440513495	26.359648236126553	22.115839482462345
14	21.771353482260185	21.7078742545234	29.112705953704637	27.408066309511774
15	23.353077933892653	29.49519862529061	25.203679369250985	21.948044071565754
16	21.686444961083595	29.004750833922976	26.235924390983524	23.072879814009905
17	21.77499241888204	27.716162943495398	27.270595370463962	23.238249267158597
18	22.861821489942383	26.761144243404427	28.869301526331746	21.50773274032144
19	24.405539270191042	26.525017689275245	28.705549378348326	20.363893662185383
20	24.49651268573739	26.241180632770643	28.10552916203376	21.156777519458203
21	23.11412109572425	27.11695137976347	28.181542504801378	21.587385019710904
22	22.14414232285454	27.29768523198221	28.986960477105022	21.571211968058222
23	22.38795107651875	26.95562518952795	28.604063479227737	22.052360254725563
24	21.940766198322045	26.98150207217224	28.923885575659558	22.153846153846153
25	22.598200748003638	27.261295865763675	28.78843626806833	21.35206711816436
26	22.588092590720713	27.29930253714748	28.282624077630647	21.829980794501164
27	22.569493581320124	26.983928029920147	28.671181643586372	21.775396745173357
28	22.19185282522996	27.089052865662588	28.542605882947537	22.17648842615991
29	22.42838370565046	27.346204386940258	28.3784494086728	21.84696249873648
30	23.034468816334783	27.061558677853025	28.159708885070252	21.74426362074194
31	22.491458607095925	27.298089558273524	28.395835439199434	21.814616395431113
32	21.573637925806125	27.231780046497523	28.74436470231477	22.450217325381583
33	21.551804306075002	27.372889922167186	29.009602749418782	22.06570302233903
34	22.17770140503386	26.680683311432325	28.89356110381078	22.248054179723038
35	21.796421712321845	27.13514606287274	28.721722430001012	22.346709794804408
36	22.096431820479125	27.538663701607195	28.55392701910442	21.81097745880926
37	22.556959466289296	27.105225917315273	28.492469422824218	21.845345193571212
38	22.633377135348226	27.14727585161225	27.96563226523805	22.253714747801475
39	22.066107348630347	27.117760032346105	28.544627514404127	22.271505104619425
40	21.933488325078336	27.00818760739917	28.73951278681896	22.318811280703528
41	21.96623875467502	26.78338218942687	28.704740725765692	22.545638330132416
42	21.790356817952087	27.268169412716066	28.764581016880626	22.176892752451227
43	22.161932679672496	27.06721924593147	28.674011927625592	22.096836146770443
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16.0
1	21.0
2	26.0
3	104.5
4	183.0
5	183.0
6	231.0
7	279.0
8	278.5
9	278.0
10	374.0
11	470.0
12	470.0
13	724.5
14	979.0
15	1638.5
16	2298.0
17	2569.5
18	2841.0
19	2841.0
20	2698.0
21	2555.0
22	2884.5
23	3214.0
24	4122.0
25	5030.0
26	5030.0
27	6012.0
28	6994.0
29	8213.0
30	9432.0
31	10619.5
32	11807.0
33	11807.0
34	12768.5
35	13730.0
36	14363.0
37	14996.0
38	15632.5
39	16269.0
40	16269.0
41	16709.0
42	17149.0
43	17063.0
44	16977.0
45	17194.0
46	17411.0
47	17411.0
48	17429.5
49	17448.0
50	17132.0
51	16816.0
52	16231.0
53	15646.0
54	15646.0
55	14611.0
56	13576.0
57	12332.5
58	11089.0
59	10295.0
60	9501.0
61	9501.0
62	8293.5
63	7086.0
64	5943.5
65	4801.0
66	4046.5
67	3292.0
68	3292.0
69	2734.0
70	2176.0
71	1826.5
72	1477.0
73	1109.0
74	741.0
75	741.0
76	518.5
77	296.0
78	227.5
79	159.0
80	130.5
81	102.0
82	102.0
83	87.0
84	72.0
85	67.0
86	62.0
87	46.5
88	31.0
89	31.0
90	23.0
91	15.0
92	10.0
93	5.0
94	2.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	247325.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.45898029311691
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.17409494628639	68.63189119141425
2	4.444410638728022	6.618525683271378
3	1.083394445970149	2.420053371064909
4	0.5653012839362955	1.6836702864114526
5	0.33031411787155607	1.2297426196568244
6	0.25425021337919085	1.1358726976513163
7	0.1765890332107693	0.9204047540674866
8	0.12316795346608167	0.7336768175899612
9	0.09903768542474393	0.6636820560585228
>10	0.6717343381342259	9.411915216614911
>50	0.057056371169373905	2.930558648103718
>100	0.0190187903897913	2.2718606667268224
>500	5.433940111368944E-4	0.24033574996184018
>1k	0.0010867880222737887	1.1078102414065967
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1530	0.6186192257151522	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1208	0.48842615991104826	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	594	0.24016981704235318	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	360	0.14555746487415344	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	345	0.13949257050439703	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	279	0.11280703527746891	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0012129788739512787	0.0	0.0
2	0.0	0.0	0.0016173051652683716	0.0	0.0
3	0.0	0.0	0.0016173051652683716	0.0	0.0
4	0.0	0.0	0.0016173051652683716	0.0	0.0
5	0.0	0.0	0.0024259577479025574	0.0	0.0
6	0.0	0.0	0.004447589204488022	0.0	0.0
7	0.0	0.0	0.00849085211765895	0.0	0.0
8	0.0	0.0	0.010512483574244415	0.0	0.0
9	0.0	0.0	0.01253411503082988	0.0012129788739512787	0.0
10	4.043262913170929E-4	0.0	0.014151420196098252	0.0012129788739512787	0.0
11	4.043262913170929E-4	0.0	0.01940766198322046	0.0012129788739512787	0.0
12	4.043262913170929E-4	0.0	0.020216314565854644	0.0012129788739512787	0.0
13	4.043262913170929E-4	0.0	0.022642272313757202	0.0012129788739512787	0.0
14	4.043262913170929E-4	0.0	0.024663903770342666	0.0012129788739512787	0.0
15	4.043262913170929E-4	0.0	0.026685535226928132	0.0012129788739512787	0.0
16	4.043262913170929E-4	0.0	0.028302840392196504	0.0012129788739512787	0.0
17	4.043262913170929E-4	0.0	0.028707166683513596	0.0016173051652683716	0.0
18	4.043262913170929E-4	0.0	0.028707166683513596	0.0016173051652683716	0.0
19	4.043262913170929E-4	0.0	0.02951581926614778	0.0016173051652683716	0.0
20	4.043262913170929E-4	0.0	0.030324471848781967	0.0016173051652683716	0.0
21	4.043262913170929E-4	0.0	0.03072879814009906	0.0016173051652683716	0.0
22	4.043262913170929E-4	0.0	0.03072879814009906	0.0016173051652683716	0.0
23	4.043262913170929E-4	0.0	0.03072879814009906	0.0020216314565854646	0.0
24	4.043262913170929E-4	0.0	0.03072879814009906	0.0024259577479025574	0.0
25	4.043262913170929E-4	0.0	0.031133124431416154	0.0028302840392196503	0.0
26	4.043262913170929E-4	0.0	0.031133124431416154	0.0028302840392196503	0.0
27	4.043262913170929E-4	0.0	0.031133124431416154	0.004043262913170929	0.0
28	4.043262913170929E-4	0.0	0.031133124431416154	0.011725462448195693	0.0
29	4.043262913170929E-4	0.0	0.031133124431416154	0.024663903770342666	0.0
30	4.043262913170929E-4	0.0	0.031133124431416154	0.052562417871222074	0.0
31	4.043262913170929E-4	0.0	0.031133124431416154	0.1310017183867381	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	290	0.0	32.53448	1
GCCGTCC	50	2.7062015E-7	29.6	27
GTCCTAG	35	8.849645E-4	26.42857	1
TCGCCGT	60	1.330327E-6	24.666666	25
CGAGTCG	60	1.330327E-6	24.666666	21
CGCCGTC	60	1.330327E-6	24.666666	26
GCGAGTC	60	1.330327E-6	24.666666	20
ACCTAGG	55	1.8948414E-5	23.545454	1
TGGGCGC	55	1.8948414E-5	23.545454	12
GCCGCTC	40	0.001926927	23.125002	27
CGGGGAA	40	0.001926927	23.125002	17
TTCGCCG	40	0.001926927	23.125002	24
ATGGGCG	65	2.6687321E-6	22.76923	11
GGCGAGT	65	2.6687321E-6	22.76923	19
CGCGGCT	50	2.6938005E-4	22.2	10
TACTGAG	50	2.6938005E-4	22.2	5
GTATCAA	825	0.0	22.199999	1
GCGCAAG	60	3.7110854E-5	21.583332	15
CGCAAGC	60	3.7110854E-5	21.583332	16
GTCCGCC	70	5.075255E-6	21.142857	30
>>END_MODULE