FastQCFastQC Report
Fri 10 Feb 2017
ERR1632804.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632804.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences368688
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16710.4532287462569978No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12690.3441934643926572No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5950.16138306644100162No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA3940.10686542550883131No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCA556.2423715E-726.9090912
GGTATCA3200.025.4375021
AACAGTG451.320831E-424.6666687
GTATCAA8600.024.0930231
ATTAGAC400.001928712923.1250023
TATTAGA1107.4578566E-1121.8636362
GCACCGC705.0864255E-621.14285710
ACCTCGA806.933751E-720.812534
CGAGTCG450.003820952720.55555721
ATCGCGC450.003820952720.5555578
GTCCGGC656.885886E-519.92307715
GAGTCGC500.007026043318.49999822
TTAGACT500.007026043318.4999984
CGAGCTC1002.8617796E-718.49999828
TCGCGCG500.007026043318.4999989
GCGAGTC500.007026043318.49999820
CGAACCG1351.1423253E-917.81481631
GTATTAG1703.6379788E-1217.4117641
GAACTAT752.0624709E-417.2666661
AACCGGG1401.8553692E-917.17857233
Produced by FastQC (version 0.11.5)