##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632804.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	368688
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.68546575966671	33.0	33.0	33.0	27.0	33.0
2	31.21472627262075	33.0	33.0	33.0	27.0	33.0
3	31.654013691793605	33.0	33.0	33.0	27.0	33.0
4	31.809386256129844	33.0	33.0	33.0	27.0	33.0
5	31.917727183960423	33.0	33.0	33.0	33.0	33.0
6	34.75436412359502	37.0	37.0	37.0	27.0	37.0
7	34.92484431280649	37.0	37.0	37.0	27.0	37.0
8	35.0860809139435	37.0	37.0	37.0	33.0	37.0
9	35.178394740268196	37.0	37.0	37.0	33.0	37.0
10	35.214834765438525	37.0	37.0	37.0	33.0	37.0
11	35.252630950831055	37.0	37.0	37.0	33.0	37.0
12	35.20683613244803	37.0	37.0	37.0	33.0	37.0
13	35.22749045263203	37.0	37.0	37.0	33.0	37.0
14	35.180955170767696	37.0	37.0	37.0	33.0	37.0
15	35.20685783101159	37.0	37.0	37.0	33.0	37.0
16	35.217723386711796	37.0	37.0	37.0	33.0	37.0
17	35.17478735407716	37.0	37.0	37.0	33.0	37.0
18	35.18064325391659	37.0	37.0	37.0	33.0	37.0
19	35.192591025474115	37.0	37.0	37.0	33.0	37.0
20	35.18420453066007	37.0	37.0	37.0	33.0	37.0
21	35.20456863255652	37.0	37.0	37.0	33.0	37.0
22	35.1941668836523	37.0	37.0	37.0	33.0	37.0
23	35.168953152801286	37.0	37.0	37.0	33.0	37.0
24	35.179403723473506	37.0	37.0	37.0	33.0	37.0
25	35.16038222019702	37.0	37.0	37.0	33.0	37.0
26	35.136256129844206	37.0	37.0	37.0	33.0	37.0
27	35.08069424554095	37.0	37.0	37.0	33.0	37.0
28	35.03023694831402	37.0	37.0	37.0	27.0	37.0
29	35.08166254393959	37.0	37.0	37.0	33.0	37.0
30	35.02876687063316	37.0	37.0	37.0	27.0	37.0
31	35.02587824935989	37.0	37.0	37.0	27.0	37.0
32	35.052355921537995	37.0	37.0	37.0	27.0	37.0
33	35.03530085058369	37.0	37.0	37.0	27.0	37.0
34	34.94573460486916	37.0	37.0	37.0	27.0	37.0
35	34.959442672395085	37.0	37.0	37.0	27.0	37.0
36	34.94512975741006	37.0	37.0	37.0	27.0	37.0
37	34.97139315627305	37.0	37.0	37.0	27.0	37.0
38	34.829159072169425	37.0	37.0	37.0	27.0	37.0
39	34.89314813609339	37.0	37.0	37.0	27.0	37.0
40	34.92524844855271	37.0	37.0	37.0	27.0	37.0
41	34.914382892852494	37.0	37.0	37.0	27.0	37.0
42	34.825717137525494	37.0	37.0	37.0	27.0	37.0
43	34.60807783274747	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	9.0
16	29.0
17	17.0
18	19.0
19	23.0
20	39.0
21	96.0
22	250.0
23	560.0
24	1174.0
25	1988.0
26	3304.0
27	4815.0
28	6865.0
29	9224.0
30	11691.0
31	14788.0
32	18630.0
33	24303.0
34	33952.0
35	61770.0
36	175141.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.253037798897715	20.638588725426377	13.739530443084668	24.368843032591244
2	15.656327301132663	23.834787137091524	38.458805277090654	22.05008028468515
3	17.775734496376337	27.47987458230265	30.957611856095124	23.786779065225883
4	12.459586425378639	18.242796076899708	40.05988803541206	29.2377294623096
5	13.530953000911339	37.21547758538385	35.75733411448162	13.49623529922319
6	28.467430456103806	40.56627826237903	17.69083886646704	13.275452415050124
7	25.359111226836784	33.031180835828664	24.00376470077681	17.60594323655774
8	23.954129236644533	36.157401380028645	21.138198151282385	18.750271232044437
9	26.016848934600528	15.469719654558869	20.323688321833096	38.18974308900751
10	14.510100681334896	28.593824588812222	34.52512693659679	22.370947793256086
11	33.2335199409799	24.137753330729506	24.5432452371653	18.08548149112529
12	22.114362279217115	26.139445818686802	30.89794080631862	20.84825109577746
13	28.010946925313547	22.531517163563773	26.79853968667274	22.658996224449943
14	21.928568328776635	21.429501367009504	28.419151152193724	28.222779152020138
15	23.62349737447381	28.907368832183312	25.203152801284556	22.265980992058328
16	22.168608688104847	28.98033025213731	25.69326910558521	23.157791954172634
17	22.028924185218937	27.7334765438528	26.818068393872323	23.419530877055937
18	22.679067395738404	27.133782493598922	28.22576270450896	21.96138740615371
19	24.463774248144773	26.65939764787571	28.17585600833225	20.70097209564727
20	24.31703771210346	26.296489172416788	28.023152367313287	21.363320748166473
21	22.50954736796424	27.504285466302132	27.671906869765223	22.314260295968406
22	22.213633207481664	27.31279564292844	27.80345441131797	22.670116738271926
23	22.28740832356898	27.227357548930257	28.266176279130324	22.21905784837044
24	22.736568589159397	27.443258256303434	27.483129366835918	22.337043787701255
25	22.925346092088702	27.010371913379334	28.190502538731938	21.873779455800026
26	22.836381981512822	27.15873584168728	27.697673913986893	22.307208262813003
27	22.914768042355597	27.024747211734585	27.557989411100987	22.502495334808835
28	22.104055461528446	27.26261771470729	28.14927526797726	22.48405155578701
29	22.96684459488782	27.28268888599575	27.50862517901315	22.241841340103285
30	22.713242633337675	27.37924749381591	27.95642928438137	21.951080588465043
31	22.876795556134184	27.03559649351213	27.810235212428935	22.27737273792475
32	21.639434969405023	27.11859349911036	28.222236687931257	23.01973484355336
33	22.046554268107453	26.98514733324654	28.438679859393307	22.529618539252702
34	22.177016881482444	26.86173675302695	28.10831922926702	22.852927136223585
35	22.454216030898756	27.32689970923925	27.969448422514432	22.249435837347566
36	22.69561255044916	27.34886950483878	27.62986590287723	22.325652041834832
37	23.08564423035195	26.813457449116868	27.44542811265894	22.655470207872238
38	22.964945970576746	27.080892244933384	27.56124419563425	22.392917588855617
39	22.439027036410188	26.881536692270974	28.08038232868984	22.599053942628995
40	22.321041097079373	27.12211951568806	28.257768085752723	22.29907130147984
41	22.405665494944234	26.652888078809184	28.289773466996486	22.651672959250096
42	22.31724384845723	26.96643232218027	28.024508527535474	22.69181530182702
43	22.33351777112355	26.591589636766045	28.180738185132142	22.89415440697826
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	15.0
2	24.0
3	100.5
4	177.0
5	177.0
6	231.5
7	286.0
8	283.5
9	281.0
10	360.5
11	440.0
12	440.0
13	663.5
14	887.0
15	1676.0
16	2465.0
17	2751.0
18	3037.0
19	3037.0
20	2912.5
21	2788.0
22	3270.0
23	3752.0
24	4894.5
25	6037.0
26	6037.0
27	7489.0
28	8941.0
29	11089.5
30	13238.0
31	14875.0
32	16512.0
33	16512.0
34	18233.0
35	19954.0
36	21446.5
37	22939.0
38	24637.0
39	26335.0
40	26335.0
41	27207.0
42	28079.0
43	27850.5
44	27622.0
45	27636.0
46	27650.0
47	27650.0
48	27346.5
49	27043.0
50	27221.5
51	27400.0
52	26892.0
53	26384.0
54	26384.0
55	23680.5
56	20977.0
57	18733.5
58	16490.0
59	14472.5
60	12455.0
61	12455.0
62	10954.0
63	9453.0
64	7588.0
65	5723.0
66	4938.5
67	4154.0
68	4154.0
69	3453.5
70	2753.0
71	2324.0
72	1895.0
73	1526.5
74	1158.0
75	1158.0
76	869.5
77	581.0
78	451.0
79	321.0
80	248.0
81	175.0
82	175.0
83	142.5
84	110.0
85	102.0
86	94.0
87	72.0
88	50.0
89	50.0
90	32.5
91	15.0
92	10.0
93	5.0
94	3.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	368688.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.80248008543704
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.06344216397025	59.26397859420563
2	5.361070909651887	7.055435235379683
3	1.4332440514286833	2.8293303945512114
4	0.6844912642353377	1.8016489113400573
5	0.4381640076637711	1.4416139194225293
6	0.29473852669175404	1.1636715619827123
7	0.21971793849839827	1.0120589690717894
8	0.18699688343126944	0.9843886958419921
9	0.12990880205243654	0.7693489223980596
>10	1.046009498163937	13.840722368977609
>50	0.09909508179815908	4.525749255544891
>100	0.04187700109449177	4.34758948558848
>500	4.146237732127898E-4	0.16233547184971103
>1k	8.292475464255796E-4	0.802128213845631
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1671	0.4532287462569978	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1269	0.3441934643926572	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	595	0.16138306644100162	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	394	0.10686542550883131	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	8.136961333159745E-4	0.0	0.0
2	0.0	0.0	8.136961333159745E-4	0.0	0.0
3	0.0	0.0	8.136961333159745E-4	0.0	0.0
4	0.0	0.0	8.136961333159745E-4	0.0	0.0
5	0.0	0.0	0.0013561602221932908	0.0	0.0
6	0.0	0.0	0.0035260165777025562	0.0	0.0
7	0.0	0.0	0.006780801110966454	0.0	0.0
8	0.0	0.0	0.00895065746647572	0.0	0.0
9	0.0	0.0	0.011120513821984984	5.424640888773163E-4	0.0
10	2.7123204443865815E-4	0.0	0.012205441999739617	5.424640888773163E-4	0.0
11	2.7123204443865815E-4	0.0	0.018443779021828755	8.136961333159745E-4	0.0
12	2.7123204443865815E-4	0.0	0.020071171288460705	0.0010849281777546326	0.0
13	2.7123204443865815E-4	0.0	0.021698563555092653	0.0010849281777546326	0.0
14	2.7123204443865815E-4	0.0	0.022783491732847286	0.0010849281777546326	0.0
15	2.7123204443865815E-4	0.0	0.024410883999479233	0.0010849281777546326	0.0
16	2.7123204443865815E-4	0.0	0.02630950831054984	0.0010849281777546326	0.0
17	2.7123204443865815E-4	0.0	0.026851972399427156	0.0010849281777546326	0.0
18	2.7123204443865815E-4	0.0	0.027665668532743132	0.0010849281777546326	0.0
19	2.7123204443865815E-4	0.0	0.028479364666059107	0.0010849281777546326	0.0
20	2.7123204443865815E-4	0.0	0.029293060799375083	0.0013561602221932908	0.0
21	2.7123204443865815E-4	0.0	0.030649221021568373	0.0013561602221932908	0.0
22	2.7123204443865815E-4	0.0	0.03092045306600703	0.0018986243110706072	0.0
23	2.7123204443865815E-4	0.0	0.03092045306600703	0.0024410883999479234	0.0
24	2.7123204443865815E-4	0.0	0.03146291715488435	0.003254784533263898	0.0
25	2.7123204443865815E-4	0.0	0.03146291715488435	0.0035260165777025562	0.0
26	2.7123204443865815E-4	0.0	0.03146291715488435	0.0037972486221412144	0.0
27	2.7123204443865815E-4	0.0	0.03146291715488435	0.005424640888773163	0.0
28	2.7123204443865815E-4	0.0	0.03146291715488435	0.0116629779108623	0.0
29	2.7123204443865815E-4	0.0	0.03146291715488435	0.028750596710497765	0.0
30	2.7123204443865815E-4	0.0	0.03146291715488435	0.055331337065486264	0.0
31	2.7123204443865815E-4	0.0	0.03146291715488435	0.12612290066397605	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGCCA	55	6.2423715E-7	26.90909	12
GGTATCA	320	0.0	25.437502	1
AACAGTG	45	1.320831E-4	24.666668	7
GTATCAA	860	0.0	24.093023	1
ATTAGAC	40	0.0019287129	23.125002	3
TATTAGA	110	7.4578566E-11	21.863636	2
GCACCGC	70	5.0864255E-6	21.142857	10
ACCTCGA	80	6.933751E-7	20.8125	34
CGAGTCG	45	0.0038209527	20.555557	21
ATCGCGC	45	0.0038209527	20.555557	8
GTCCGGC	65	6.885886E-5	19.923077	15
GAGTCGC	50	0.0070260433	18.499998	22
TTAGACT	50	0.0070260433	18.499998	4
CGAGCTC	100	2.8617796E-7	18.499998	28
TCGCGCG	50	0.0070260433	18.499998	9
GCGAGTC	50	0.0070260433	18.499998	20
CGAACCG	135	1.1423253E-9	17.814816	31
GTATTAG	170	3.6379788E-12	17.411764	1
GAACTAT	75	2.0624709E-4	17.266666	1
AACCGGG	140	1.8553692E-9	17.178572	33
>>END_MODULE