FastQCFastQC Report
Fri 10 Feb 2017
ERR1632803.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632803.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences338737
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12140.35839013748129084No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8460.2497512819680165No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4490.1325512122974461No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA5850.024.6666681
TAGGACC701.9145955E-723.7857154
GGTATCA1750.023.2571431
TTAGCCG855.1535608E-821.76470629
TTCGCCG603.7163067E-521.58333224
CTACGGT450.003820321320.55555727
TCTAACC450.003820321320.5555573
GCGAGTC450.003820321320.55555720
GAGGCGT450.003820321320.55555729
TCCGCCG555.132807E-420.18181831
CGCGCGC656.883778E-519.92307710
GCTTCGC656.883778E-519.92307722
TCGCGCG656.883778E-519.9230779
ATTACAC609.218492E-418.53
CGCGCCT701.21597805E-418.512
TAGCCGG1002.8600698E-718.530
GCCGCTC609.218492E-418.527
CGCCTTT609.218492E-418.514
CCCTTAG500.007024890718.51
TCCCGGA500.007024890718.527
Produced by FastQC (version 0.11.5)