##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632803.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	338737
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.598744748875973	33.0	33.0	33.0	27.0	33.0
2	31.162297003279832	33.0	33.0	33.0	27.0	33.0
3	31.59480954250643	33.0	33.0	33.0	27.0	33.0
4	31.742584955289797	33.0	33.0	33.0	27.0	33.0
5	31.837732518148297	33.0	33.0	33.0	33.0	33.0
6	34.70000029521428	37.0	37.0	37.0	27.0	37.0
7	34.8389724181297	37.0	37.0	37.0	27.0	37.0
8	35.03115691524693	37.0	37.0	37.0	33.0	37.0
9	35.08134629520838	37.0	37.0	37.0	33.0	37.0
10	35.15062718274059	37.0	37.0	37.0	33.0	37.0
11	35.185506750074545	37.0	37.0	37.0	33.0	37.0
12	35.11902449392892	37.0	37.0	37.0	33.0	37.0
13	35.14868467276973	37.0	37.0	37.0	33.0	37.0
14	35.12179360388738	37.0	37.0	37.0	33.0	37.0
15	35.14317006999531	37.0	37.0	37.0	33.0	37.0
16	35.14160248216168	37.0	37.0	37.0	33.0	37.0
17	35.114856068277156	37.0	37.0	37.0	33.0	37.0
18	35.094991099289416	37.0	37.0	37.0	33.0	37.0
19	35.11245302402749	37.0	37.0	37.0	33.0	37.0
20	35.0705178353708	37.0	37.0	37.0	33.0	37.0
21	35.120323436766576	37.0	37.0	37.0	33.0	37.0
22	35.13082125660911	37.0	37.0	37.0	33.0	37.0
23	35.096685629264	37.0	37.0	37.0	33.0	37.0
24	35.085857169426426	37.0	37.0	37.0	33.0	37.0
25	35.10457670700278	37.0	37.0	37.0	33.0	37.0
26	35.07279688962233	37.0	37.0	37.0	33.0	37.0
27	34.995908330061376	37.0	37.0	37.0	27.0	37.0
28	34.97697328605969	37.0	37.0	37.0	27.0	37.0
29	35.016177742614474	37.0	37.0	37.0	27.0	37.0
30	34.99284695796444	37.0	37.0	37.0	27.0	37.0
31	34.97436654395593	37.0	37.0	37.0	27.0	37.0
32	35.009443904858344	37.0	37.0	37.0	27.0	37.0
33	34.966546317644664	37.0	37.0	37.0	27.0	37.0
34	34.91610305340131	37.0	37.0	37.0	27.0	37.0
35	34.90925112993266	37.0	37.0	37.0	27.0	37.0
36	34.87503579473161	37.0	37.0	37.0	27.0	37.0
37	34.913702961294454	37.0	37.0	37.0	27.0	37.0
38	34.76165284571807	37.0	37.0	37.0	27.0	37.0
39	34.83242751751359	37.0	37.0	37.0	27.0	37.0
40	34.88558380100196	37.0	37.0	37.0	27.0	37.0
41	34.890248186646275	37.0	37.0	37.0	27.0	37.0
42	34.754092407974326	37.0	37.0	37.0	27.0	37.0
43	34.580341090580596	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	14.0
16	25.0
17	25.0
18	14.0
19	14.0
20	39.0
21	89.0
22	225.0
23	540.0
24	1152.0
25	1945.0
26	3164.0
27	4667.0
28	6524.0
29	8712.0
30	11061.0
31	13928.0
32	17886.0
33	22814.0
34	32463.0
35	58679.0
36	154756.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.03832767013937	20.216273982470177	13.38442508494791	25.360973262442542
2	16.144678614972676	23.118820796074832	37.35228215400148	23.38421843495101
3	18.11346265686949	26.535335673398535	30.216952975317135	25.13424869441484
4	12.874885235448149	17.889099803092073	38.93492591597611	30.301089045483664
5	14.387858427039266	36.70162987804698	34.94510490439485	13.965406790518898
6	29.85797240927327	39.634583762624096	17.31372716886552	13.193716659237106
7	26.59437852965575	31.997095091472143	23.457431576709954	17.95109480216215
8	24.728624271927778	35.558560180907314	20.782199759695573	18.930615787469335
9	26.16218482185294	15.049728845682639	19.869101987677755	38.91898434478666
10	14.67451149416804	28.25820621898405	34.15658755907976	22.91069472776815
11	34.82524790619271	23.112326081886536	24.01450092549677	18.04792508642398
12	22.785523872502857	25.15993233688673	30.7772696811981	21.277274109412318
13	28.586484499774162	21.76703460206591	26.00896860986547	23.637512288294456
14	22.241443952092627	20.632821333364824	27.779959083300614	29.34577563124194
15	24.09952263850716	28.52714642923566	24.113397709727604	23.259933222529572
16	22.975051441088514	28.04151893652007	25.22901247870767	23.754417143683742
17	22.678951516958584	27.339204161340508	26.45621824601387	23.525626075687036
18	23.500237647496437	26.70095088520002	27.430425374257904	22.368386093045636
19	24.59341613109876	26.423154246509828	26.925313738977437	22.058115883413976
20	24.471197418646323	26.15391882197693	26.882507668190957	22.492376091185786
21	23.40222650610946	26.6230143149405	27.142591450003984	22.832167728946057
22	22.926636298957597	26.61091052940777	27.23735523429681	23.225097937337814
23	23.16989286673732	26.41046003241453	27.332709447152215	23.086937653695934
24	23.319861721630648	26.738443098923355	26.589655101155174	23.352040078290827
25	23.61182864582257	26.584931672654594	27.28281823361487	22.520421447907964
26	23.752645857996026	26.412231318102247	26.937712738791454	22.897410085110277
27	23.3907131491393	26.776820955490543	26.885459811003816	22.947006084366336
28	23.063615725474335	26.69534181385559	27.180969306571175	23.0600731540989
29	23.61920900285472	26.633642029066802	26.736671813235635	23.010477154842842
30	23.473963576461976	26.575189601372156	27.03483823733457	22.9160085848313
31	23.477210933556123	26.37001567587834	26.94214095301074	23.2106324375548
32	22.95438644139849	26.434667603479983	27.10303273631165	23.507913218809872
33	22.937264013083897	26.46743638870274	27.545854158240758	23.049445439972605
34	23.181701437988764	26.47363588860975	27.071740022495327	23.27292265090616
35	23.063615725474335	26.787448669616843	27.12871637878354	23.02021922612528
36	23.70954457292826	26.435258032042558	26.821988740527313	23.03320865450187
37	23.57640293206824	26.34344639056259	26.866861311282797	23.21328936608637
38	23.33521286425752	26.60264452953176	26.869518239814372	23.19262436639635
39	22.874973799732537	26.36322574740876	27.309682733211904	23.452117719646807
40	23.010477154842842	26.61533874362706	27.46083244523037	22.913351656299724
41	23.17077850958118	26.04793689499523	27.458765945261366	23.32251865016222
42	22.95586251280492	26.63954631469252	27.281932590771014	23.12265858173155
43	23.173435438112755	26.05354596633967	27.43160623138305	23.34141236416453
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9.0
1	12.0
2	15.0
3	81.0
4	147.0
5	147.0
6	176.0
7	205.0
8	187.5
9	170.0
10	248.5
11	327.0
12	327.0
13	503.0
14	679.0
15	1155.5
16	1632.0
17	1914.5
18	2197.0
19	2197.0
20	2058.0
21	1919.0
22	2264.0
23	2609.0
24	3463.5
25	4318.0
26	4318.0
27	5598.0
28	6878.0
29	8331.0
30	9784.0
31	11549.5
32	13315.0
33	13315.0
34	15250.0
35	17185.0
36	18455.5
37	19726.0
38	21175.0
39	22624.0
40	22624.0
41	23680.0
42	24736.0
43	25419.0
44	26102.0
45	26140.0
46	26178.0
47	26178.0
48	26185.5
49	26193.0
50	25751.0
51	25309.0
52	24396.5
53	23484.0
54	23484.0
55	21986.5
56	20489.0
57	18520.5
58	16552.0
59	15356.5
60	14161.0
61	14161.0
62	12450.0
63	10739.0
64	9000.5
65	7262.0
66	6177.5
67	5093.0
68	5093.0
69	4302.5
70	3512.0
71	2838.0
72	2164.0
73	1756.5
74	1349.0
75	1349.0
76	1029.0
77	709.0
78	544.5
79	380.0
80	312.0
81	244.0
82	244.0
83	200.0
84	156.0
85	119.5
86	83.0
87	78.0
88	73.0
89	73.0
90	49.5
91	26.0
92	15.0
93	4.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	338737.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.0647229897932
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.5182277999915	65.40624516743583
2	6.182087768023663	9.033850605384758
3	1.6377851109724988	3.5899294635004004
4	0.7688399684830584	2.2470031728278395
5	0.4050057937807729	1.479581806592674
6	0.2717837681496747	1.19146834397869
7	0.2018276615817485	1.0322537529603726
8	0.14756885045770932	0.8625661744491799
9	0.11605870106033911	0.7631817159176005
>10	0.6792401044198382	9.099278269783431
>50	0.05205416223951256	2.674508579950357
>100	0.018706964554734758	2.008035660842066
>500	4.066731424942338E-4	0.2513758758615549
>1k	4.066731424942338E-4	0.3607214105152809
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1214	0.35839013748129084	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	846	0.2497512819680165	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	449	0.1325512122974461	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	5.90428562572143E-4	0.0	0.0
2	0.0	0.0	5.90428562572143E-4	0.0	0.0
3	0.0	0.0	5.90428562572143E-4	0.0	0.0
4	0.0	0.0	8.856428438582145E-4	0.0	0.0
5	0.0	0.0	0.001180857125144286	0.0	0.0
6	0.0	0.0	0.002361714250288572	0.0	0.0
7	0.0	0.0	0.005018642781863215	0.0	0.0
8	0.0	0.0	0.007675571313437859	0.0	0.0
9	2.952142812860715E-4	0.0	0.007970785594723931	2.952142812860715E-4	0.0
10	2.952142812860715E-4	0.0	0.008561214157296073	2.952142812860715E-4	0.0
11	2.952142812860715E-4	0.0	0.012398999814015002	2.952142812860715E-4	0.0
12	2.952142812860715E-4	0.0	0.014760714064303574	2.952142812860715E-4	0.0
13	2.952142812860715E-4	0.0	0.016827214033306077	2.952142812860715E-4	0.0
14	2.952142812860715E-4	0.0	0.02007457112745286	5.90428562572143E-4	0.0
15	2.952142812860715E-4	0.0	0.022436285377741432	5.90428562572143E-4	0.0
16	2.952142812860715E-4	0.0	0.02361714250288572	5.90428562572143E-4	0.0
17	2.952142812860715E-4	0.0	0.023912356784171793	8.856428438582145E-4	0.0
18	2.952142812860715E-4	0.0	0.024797999628030004	8.856428438582145E-4	0.0
19	2.952142812860715E-4	0.0	0.02568364247188822	8.856428438582145E-4	0.0
20	2.952142812860715E-4	0.0	0.026274071034460365	8.856428438582145E-4	0.0
21	2.952142812860715E-4	0.0	0.026864499597032507	8.856428438582145E-4	0.0
22	2.952142812860715E-4	0.0	0.02745492815960465	8.856428438582145E-4	0.0
23	2.952142812860715E-4	0.0	0.02804535672217679	0.0014760714064303575	0.0
24	2.952142812860715E-4	0.0	0.028340571003462864	0.001771285687716429	0.0
25	2.952142812860715E-4	0.0	0.028635785284748937	0.0020664999690025005	0.0
26	2.952142812860715E-4	0.0	0.028635785284748937	0.0020664999690025005	0.0
27	2.952142812860715E-4	0.0	0.028635785284748937	0.0032473570941467865	0.0
28	2.952142812860715E-4	0.0	0.028635785284748937	0.012398999814015002	0.0
29	2.952142812860715E-4	0.0	0.028635785284748937	0.026274071034460365	0.0
30	2.952142812860715E-4	0.0	0.028635785284748937	0.04900557069348787	0.0
31	2.952142812860715E-4	0.0	0.028635785284748937	0.11483835542028181	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	585	0.0	24.666668	1
TAGGACC	70	1.9145955E-7	23.785715	4
GGTATCA	175	0.0	23.257143	1
TTAGCCG	85	5.1535608E-8	21.764706	29
TTCGCCG	60	3.7163067E-5	21.583332	24
CTACGGT	45	0.0038203213	20.555557	27
TCTAACC	45	0.0038203213	20.555557	3
GCGAGTC	45	0.0038203213	20.555557	20
GAGGCGT	45	0.0038203213	20.555557	29
TCCGCCG	55	5.132807E-4	20.181818	31
CGCGCGC	65	6.883778E-5	19.923077	10
GCTTCGC	65	6.883778E-5	19.923077	22
TCGCGCG	65	6.883778E-5	19.923077	9
ATTACAC	60	9.218492E-4	18.5	3
CGCGCCT	70	1.21597805E-4	18.5	12
TAGCCGG	100	2.8600698E-7	18.5	30
GCCGCTC	60	9.218492E-4	18.5	27
CGCCTTT	60	9.218492E-4	18.5	14
CCCTTAG	50	0.0070248907	18.5	1
TCCCGGA	50	0.0070248907	18.5	27
>>END_MODULE