FastQCFastQC Report
Fri 10 Feb 2017
ERR1632802.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632802.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304030
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18170.5976383909482617No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13560.4460086175706345No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7630.25096207611091015No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4130.13584185771141005No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA3690.12136960168404433No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGCTT250.005490064629.617
GGTATCA3700.029.51
TAGGACC405.9259877E-527.754
CGTAAAG358.854336E-426.4285728
GGCTACT358.854336E-426.4285735
CGAATTA400.001927939623.12515
CGAGCCC400.001927939623.12515
AGGTGTC400.001927939623.1257
GTATCAA8700.020.839081
ATAGAGC450.003819434220.5555573
GTAAAGC450.003819434220.55555729
GGTGTCC555.131144E-420.1818188
TTAGGAC656.8808105E-519.9230773
GCCCCGA500.007023275418.511
ACGGGTA500.007023275418.516
CCCCGAA500.007023275418.512
CCGAATT500.007023275418.514
CGGACTC500.007023275418.528
ATTAACC1054.7686444E-717.6190473
AGTCTTA650.001576323117.07692327
Produced by FastQC (version 0.11.5)