FastQCFastQC Report
Fri 10 Feb 2017
ERR1632801.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632801.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences289358
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14100.48728564615459047No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10300.35596043655264414No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4790.1655388826298219No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT3580.12372217115130739No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC3370.1164647253575156No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC3260.1126632061321961No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG3090.10678813096579325No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT3000.1036777970541682No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCAT250.00548963229.66
GGTATCA1850.028.01
GCCGCTC358.853296E-426.42857227
GAGTCGC509.060426E-625.90000222
CACGTAG659.341056E-825.61538526
GTATCAA5700.024.6666661
TCACGTA701.9125946E-723.78571525
CGAGTCG551.8962875E-523.54545421
AGCTTCG551.8962875E-523.54545421
GCTTCGC551.8962875E-523.54545422
CGCTCTC400.00192771623.12529
GGGGTTA400.00192771623.1256
TCCGCCG652.6713424E-622.7692331
CGTTGGT502.6953363E-422.224
CGCCGTC502.6953363E-422.226
GCCGTCC603.713896E-521.58333427
TCCGGCG705.080181E-621.14285916
CGTAGGA806.9231464E-720.812528
ACGTAGG806.9231464E-720.812527
CTAAACA450.003818996320.5555554