##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632801.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	289358
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.68252821764043	33.0	33.0	33.0	27.0	33.0
2	31.204746369549138	33.0	33.0	33.0	27.0	33.0
3	31.645829733409826	33.0	33.0	33.0	27.0	33.0
4	31.7673850386027	33.0	33.0	33.0	27.0	33.0
5	31.895879844345068	33.0	33.0	33.0	33.0	33.0
6	34.76426433691137	37.0	37.0	37.0	27.0	37.0
7	34.94882118344749	37.0	37.0	37.0	27.0	37.0
8	35.11426675605997	37.0	37.0	37.0	33.0	37.0
9	35.19080170584535	37.0	37.0	37.0	33.0	37.0
10	35.23557669046648	37.0	37.0	37.0	33.0	37.0
11	35.26574692940925	37.0	37.0	37.0	33.0	37.0
12	35.20908701331914	37.0	37.0	37.0	33.0	37.0
13	35.23688648663593	37.0	37.0	37.0	33.0	37.0
14	35.20943606190256	37.0	37.0	37.0	33.0	37.0
15	35.2271891566848	37.0	37.0	37.0	33.0	37.0
16	35.21236323170605	37.0	37.0	37.0	33.0	37.0
17	35.189955003836076	37.0	37.0	37.0	33.0	37.0
18	35.19405373274628	37.0	37.0	37.0	33.0	37.0
19	35.19424035278099	37.0	37.0	37.0	33.0	37.0
20	35.22287961625391	37.0	37.0	37.0	33.0	37.0
21	35.21154763303589	37.0	37.0	37.0	33.0	37.0
22	35.202966567366374	37.0	37.0	37.0	33.0	37.0
23	35.189415879291396	37.0	37.0	37.0	33.0	37.0
24	35.190604718030954	37.0	37.0	37.0	33.0	37.0
25	35.18977529565452	37.0	37.0	37.0	33.0	37.0
26	35.149216541446926	37.0	37.0	37.0	33.0	37.0
27	35.08093780023362	37.0	37.0	37.0	33.0	37.0
28	35.0585122927308	37.0	37.0	37.0	33.0	37.0
29	35.0785393871951	37.0	37.0	37.0	33.0	37.0
30	35.047249428044154	37.0	37.0	37.0	27.0	37.0
31	35.024246780804404	37.0	37.0	37.0	27.0	37.0
32	35.06372037406949	37.0	37.0	37.0	27.0	37.0
33	35.034960153166665	37.0	37.0	37.0	27.0	37.0
34	34.986273059670026	37.0	37.0	37.0	27.0	37.0
35	34.96850959710808	37.0	37.0	37.0	27.0	37.0
36	34.955200823892895	37.0	37.0	37.0	27.0	37.0
37	34.9563620152199	37.0	37.0	37.0	27.0	37.0
38	34.81687736298979	37.0	37.0	37.0	27.0	37.0
39	34.88895071157528	37.0	37.0	37.0	27.0	37.0
40	34.925479855404035	37.0	37.0	37.0	27.0	37.0
41	34.931285812039064	37.0	37.0	37.0	27.0	37.0
42	34.839541329425835	37.0	37.0	37.0	27.0	37.0
43	34.60476641392324	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	10.0
16	20.0
17	16.0
18	13.0
19	15.0
20	20.0
21	59.0
22	188.0
23	425.0
24	884.0
25	1543.0
26	2541.0
27	3687.0
28	5328.0
29	7067.0
30	9237.0
31	11703.0
32	14789.0
33	18992.0
34	26839.0
35	48770.0
36	137212.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.04189274186302	20.491916587756343	13.73661692436359	24.729573746017046
2	15.5675668203402	23.759840750903724	38.58127302511076	22.09131940364531
3	17.72130025781212	27.298363964362487	31.210818432529948	23.769517345295448
4	12.73474381216348	18.285307473786798	39.11590486525343	29.8640438487963
5	13.626718459486172	37.4238832173294	35.41391632510592	13.535481998078506
6	28.55908597654117	40.091858528189995	17.811845533906094	13.537209961362743
7	25.567635938871568	32.88245011369998	23.788524941422047	17.7613890060064
8	23.691758997504824	36.33699431154487	21.231830466066256	18.739416224884053
9	25.734557192128783	15.605927605250244	20.284560993648007	38.374954208972966
10	14.551178816552508	28.644792955439286	34.349490942016466	22.454537285991748
11	33.34900020044374	24.168676863953994	24.36393671507268	18.118386220529587
12	22.132099337153285	26.076348329750687	30.63817140013409	21.153380932961937
13	27.80673076258476	22.42412513218919	26.940675564525606	22.82846854070045
14	21.840764727431072	21.47201736257508	28.08907996322894	28.598137946764908
15	23.90740881537749	28.648248882007753	25.185756053055385	22.25858624955937
16	22.56720049212394	28.77231664581591	25.25660254770907	23.40388031435108
17	22.21469598213977	27.769061162988407	26.343491453493595	23.67275140137822
18	22.34291085783009	27.459064549796448	28.037586657358705	22.160437935014755
19	24.761713863103836	26.455809066968943	28.159580865225774	20.622896204701444
20	24.424069837364097	25.877632552063535	28.132279045334847	21.566018565237528
21	22.424816317502884	27.356769123369666	27.499844483304418	22.71857007582303
22	22.14661422874087	27.29801837170564	27.531984600391212	23.023382799162285
23	22.14004796826077	27.3277393401945	28.085624036660466	22.446588654884263
24	23.158509527989548	27.221296801885554	27.16911231070162	22.451081359423274
25	23.09111895990434	26.871557033156158	28.163382384451097	21.873941622488406
26	22.9774189758016	27.02741932139426	27.344673380380012	22.650488322424124
27	22.968433566723576	26.8791600716068	27.310805300008983	22.84160106166064
28	22.012524277884143	27.307694966097362	27.99680672385073	22.682974032167767
29	23.235231097809635	27.21300257812122	27.20574513232743	22.34602119174172
30	22.625605651131124	27.551337789174656	27.724479710255117	22.0985768494391
31	23.03893446872041	26.91890322714423	27.705126521471673	22.33703578266369
32	21.66762280635061	27.0474636954914	27.892783334139715	23.392130164018273
33	22.260659805500453	26.782048535032725	28.290214889514026	22.667076769952793
34	22.262387768784688	26.75025401060278	27.964666606763938	23.02269161384859
35	22.727901077557906	27.39754905687764	27.568963014673862	22.305586850890595
36	22.817409575681335	27.215076134062304	27.53440374898914	22.43311054126722
37	23.2452532848582	26.52458200568154	27.45768217916906	22.772482530291196
38	23.156781564705316	26.812115096178434	27.346055751007402	22.685047588108848
39	22.535405967693997	26.654179251999256	27.892783334139715	22.91763144616703
40	22.636664616150238	27.033639989217512	27.912482115580005	22.41721327905225
41	22.277939438342813	26.78446768363066	28.073873886327664	22.863718991698864
42	22.65878254618846	26.909572225409356	27.61354446740716	22.81810076099503
43	22.53747952363508	26.376322755894083	27.92319548794227	23.163002232528562
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	12.5
2	18.0
3	73.0
4	128.0
5	128.0
6	166.5
7	205.0
8	203.5
9	202.0
10	293.5
11	385.0
12	385.0
13	576.0
14	767.0
15	1394.5
16	2022.0
17	2266.5
18	2511.0
19	2511.0
20	2254.0
21	1997.0
22	2326.5
23	2656.0
24	3342.5
25	4029.0
26	4029.0
27	5146.5
28	6264.0
29	8035.5
30	9807.0
31	10919.0
32	12031.0
33	12031.0
34	13531.0
35	15031.0
36	16182.5
37	17334.0
38	18922.5
39	20511.0
40	20511.0
41	21813.5
42	23116.0
43	22332.5
44	21549.0
45	21854.5
46	22160.0
47	22160.0
48	22000.5
49	21841.0
50	22204.0
51	22567.0
52	22668.5
53	22770.0
54	22770.0
55	19770.0
56	16770.0
57	14801.0
58	12832.0
59	11164.5
60	9497.0
61	9497.0
62	8275.5
63	7054.0
64	5745.0
65	4436.0
66	3841.5
67	3247.0
68	3247.0
69	2686.5
70	2126.0
71	1804.0
72	1482.0
73	1216.5
74	951.0
75	951.0
76	715.5
77	480.0
78	368.0
79	256.0
80	194.5
81	133.0
82	133.0
83	108.5
84	84.0
85	75.0
86	66.0
87	45.5
88	25.0
89	25.0
90	17.5
91	10.0
92	5.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	289358.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.73307695887673
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.30179993384537	54.23573087948444
2	5.385822408051571	6.541951335900778
3	1.616322252572668	2.944926712675225
4	0.8092073187046215	1.9658260145029631
5	0.4990850904453522	1.5155486603522768
6	0.33160555991131807	1.2083655594051295
7	0.2543186665352232	1.0811888602733835
8	0.18867586963177896	0.9167092888503859
9	0.1548568047704633	0.8464437217557267
>10	1.2427606480262028	15.279881887525029
>50	0.14673014632294634	6.135722277026984
>100	0.06767785818771556	6.484923764421392
>500	0.0	0.0
>1k	0.001137442994751522	0.8427810378262688
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1410	0.48728564615459047	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1030	0.35596043655264414	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	479	0.1655388826298219	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	358	0.12372217115130739	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	337	0.1164647253575156	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	326	0.1126632061321961	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	309	0.10678813096579325	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	300	0.1036777970541682	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	3.455926568472273E-4	0.0	0.0
2	0.0	0.0	6.911853136944546E-4	0.0	0.0
3	0.0	0.0	6.911853136944546E-4	0.0	0.0
4	0.0	0.0	0.0013823706273889092	0.0	0.0
5	0.0	0.0	0.002419148597930591	0.0	0.0
6	0.0	0.0	0.004492704539013955	0.0	0.0
7	0.0	0.0	0.009331001734875137	0.0	0.0
8	0.0	0.0	0.01036777970541682	0.0	0.0
9	0.0	0.0	0.012441335646500183	0.0	0.0
10	3.455926568472273E-4	0.0	0.015551669558125229	3.455926568472273E-4	0.0
11	3.455926568472273E-4	0.0	0.025228263949847594	3.455926568472273E-4	0.0
12	3.455926568472273E-4	0.0	0.02730181989093096	3.455926568472273E-4	0.0
13	3.455926568472273E-4	0.0	0.02799300520462541	3.455926568472273E-4	0.0
14	3.455926568472273E-4	0.0	0.02937537583201432	3.455926568472273E-4	0.0
15	3.455926568472273E-4	0.0	0.030412153802556	3.455926568472273E-4	0.0
16	3.455926568472273E-4	0.0	0.03248570974363937	3.455926568472273E-4	0.0
17	3.455926568472273E-4	0.0	0.03386808037102828	3.455926568472273E-4	0.0
18	3.455926568472273E-4	0.0	0.03490485834156996	3.455926568472273E-4	0.0
19	3.455926568472273E-4	0.0	0.03559604365526441	3.455926568472273E-4	0.0
20	3.455926568472273E-4	0.0	0.03628722896895887	3.455926568472273E-4	0.0
21	3.455926568472273E-4	0.0	0.03836078491004223	3.455926568472273E-4	0.0
22	3.455926568472273E-4	0.0	0.03870637756688946	0.0017279632842361365	0.0
23	3.455926568472273E-4	0.0	0.03870637756688946	0.0027647412547778184	0.0
24	3.455926568472273E-4	0.0	0.03870637756688946	0.0027647412547778184	0.0
25	3.455926568472273E-4	0.0	0.03870637756688946	0.0031103339116250457	0.0
26	3.455926568472273E-4	0.0	0.03870637756688946	0.0038015192253195	0.0
27	3.455926568472273E-4	0.0	0.03870637756688946	0.005529482509555637	0.0
28	3.455926568472273E-4	0.0	0.03870637756688946	0.012441335646500183	0.0
29	3.455926568472273E-4	0.0	0.03870637756688946	0.030066561145708777	0.0
30	3.455926568472273E-4	0.0	0.03870637756688946	0.04734619398807014	0.0
31	3.455926568472273E-4	0.0	0.03870637756688946	0.11715591067121006	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCAT	25	0.005489632	29.6	6
GGTATCA	185	0.0	28.0	1
GCCGCTC	35	8.853296E-4	26.428572	27
GAGTCGC	50	9.060426E-6	25.900002	22
CACGTAG	65	9.341056E-8	25.615385	26
GTATCAA	570	0.0	24.666666	1
TCACGTA	70	1.9125946E-7	23.785715	25
CGAGTCG	55	1.8962875E-5	23.545454	21
AGCTTCG	55	1.8962875E-5	23.545454	21
GCTTCGC	55	1.8962875E-5	23.545454	22
CGCTCTC	40	0.001927716	23.125	29
GGGGTTA	40	0.001927716	23.125	6
TCCGCCG	65	2.6713424E-6	22.76923	31
CGTTGGT	50	2.6953363E-4	22.2	24
CGCCGTC	50	2.6953363E-4	22.2	26
GCCGTCC	60	3.713896E-5	21.583334	27
TCCGGCG	70	5.080181E-6	21.142859	16
CGTAGGA	80	6.9231464E-7	20.8125	28
ACGTAGG	80	6.9231464E-7	20.8125	27
CTAAACA	45	0.0038189963	20.555555	4
>>END_MODULE