FastQCFastQC Report
Fri 10 Feb 2017
ERR1632800.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632800.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences376283
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8860.2354610758391955No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6350.16875596293215478No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC4560.12118538440482297No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC4550.12091962698288256No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG4300.11427569143437252No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTG451.320902E-424.6666685
GTATTAT802.7133865E-823.1250021
GGTATCA1850.023.0000021
TAACTAG753.7269456E-722.226
GTATCAA5650.021.6106191
TGGACTA450.00382109720.5555575
GATGGGT450.00382109720.55555732
CCGAAAA450.00382109720.55555721
CGGTAAT656.886371E-519.9230771
ATTAGAG1052.2473614E-819.380953
CGGAATT500.007026302618.529
CGAACCG500.007026302618.531
ACGGGAT500.007026302618.515
TATTATA1205.140464E-918.52
CTGATAC701.2164333E-418.53
ATCGCGC609.2210836E-418.58
TACCGCA609.2210836E-418.57
TCACGTA902.1443957E-618.525
TAACGGC1156.3710104E-817.69565236
TTAACGG1156.3710104E-817.69565235
Produced by FastQC (version 0.11.5)