FastQCFastQC Report
Fri 10 Feb 2017
ERR1632799.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632799.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446686
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20990.46990503396121663No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15270.3418508751113758No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8120.18178317654907475No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA7130.15961995674814075No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG6910.15469479679237763No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6780.1517844750003358No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT5870.13141222245604295No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5040.11283093716839122No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG4910.10992061537634938No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAAC451.3214431E-424.6666683
GGTATCA4350.024.6666661
CGTTACT400.001929349323.12517
GTATCAA11600.022.6465511
AACGTCA1155.456968E-1222.5217428
TTATACC502.6985194E-422.24
GCCGTCC909.44583E-820.55555727
CGCCGTC1001.2845703E-820.3526
ACGGGTT555.136331E-420.18181816
CTGCGGG1303.274181E-1119.92307720
AACGGCC656.890063E-519.92307737
GGCGAGT1153.0358933E-919.30434819
TGCGGGT1254.0927262E-1019.24000221
GCGGGTA1254.0927262E-1019.24000222
TCTGCGG1306.9303496E-1018.519
ACTGATC701.21708064E-418.58
AAGGTCG500.00702831418.535
CCGCTCT1103.835885E-818.528
CGAGGGT1103.835885E-818.516
TTAACGG701.21708064E-418.535
Produced by FastQC (version 0.11.5)