##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632799.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	446686
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.70554707333563	33.0	33.0	33.0	27.0	33.0
2	31.223792552262662	33.0	33.0	33.0	27.0	33.0
3	31.664997335936206	33.0	33.0	33.0	27.0	33.0
4	31.80281002762567	33.0	33.0	33.0	27.0	33.0
5	31.9264181102609	33.0	33.0	33.0	33.0	33.0
6	34.77965953712451	37.0	37.0	37.0	27.0	37.0
7	34.95031856830077	37.0	37.0	37.0	27.0	37.0
8	35.12028807708323	37.0	37.0	37.0	33.0	37.0
9	35.21921663092194	37.0	37.0	37.0	33.0	37.0
10	35.239772457610044	37.0	37.0	37.0	33.0	37.0
11	35.26206999995522	37.0	37.0	37.0	33.0	37.0
12	35.209520781936305	37.0	37.0	37.0	33.0	37.0
13	35.23883443850938	37.0	37.0	37.0	33.0	37.0
14	35.212735568161975	37.0	37.0	37.0	33.0	37.0
15	35.230501963347855	37.0	37.0	37.0	33.0	37.0
16	35.24009483171624	37.0	37.0	37.0	33.0	37.0
17	35.18352041478801	37.0	37.0	37.0	33.0	37.0
18	35.19891825577699	37.0	37.0	37.0	33.0	37.0
19	35.204602338107755	37.0	37.0	37.0	33.0	37.0
20	35.21616527045844	37.0	37.0	37.0	33.0	37.0
21	35.180269809217215	37.0	37.0	37.0	33.0	37.0
22	35.198154855983844	37.0	37.0	37.0	33.0	37.0
23	35.188004549056835	37.0	37.0	37.0	33.0	37.0
24	35.18795977487542	37.0	37.0	37.0	33.0	37.0
25	35.16970757982117	37.0	37.0	37.0	33.0	37.0
26	35.1390596526419	37.0	37.0	37.0	33.0	37.0
27	35.09255718782321	37.0	37.0	37.0	33.0	37.0
28	35.06033992558531	37.0	37.0	37.0	27.0	37.0
29	35.10232467549912	37.0	37.0	37.0	33.0	37.0
30	35.06364426017382	37.0	37.0	37.0	33.0	37.0
31	35.07062903247471	37.0	37.0	37.0	27.0	37.0
32	35.08989312402896	37.0	37.0	37.0	33.0	37.0
33	35.06833211696807	37.0	37.0	37.0	33.0	37.0
34	35.00974286187613	37.0	37.0	37.0	27.0	37.0
35	35.00519380504426	37.0	37.0	37.0	27.0	37.0
36	34.982808953045314	37.0	37.0	37.0	27.0	37.0
37	35.007519823768824	37.0	37.0	37.0	27.0	37.0
38	34.8589926704665	37.0	37.0	37.0	27.0	37.0
39	34.936485584952294	37.0	37.0	37.0	27.0	37.0
40	34.95109987776649	37.0	37.0	37.0	27.0	37.0
41	34.96952445341918	37.0	37.0	37.0	27.0	37.0
42	34.87001159651299	37.0	37.0	37.0	27.0	37.0
43	34.65369409383773	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	13.0
16	20.0
17	26.0
18	22.0
19	20.0
20	35.0
21	119.0
22	259.0
23	594.0
24	1239.0
25	2362.0
26	3713.0
27	5777.0
28	8039.0
29	10877.0
30	14018.0
31	18060.0
32	22853.0
33	29574.0
34	41621.0
35	74611.0
36	212832.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.09601375462853	19.73690690999942	13.500311180560843	24.66676815481121
2	16.28705623189444	23.14758017936537	37.71821816667637	22.84714542206382
3	18.429724683558472	26.654741809682864	30.125188611239217	24.790344895519446
4	12.7680294435017	17.89915063377854	38.96719395727647	30.36562596544329
5	14.603323139744697	36.32283080284584	34.430673896204496	14.643172161204962
6	29.653940351835516	39.476724141790875	17.118065038975924	13.751270467397681
7	26.793541772072555	32.38247896732828	22.43231263124432	18.391666629354848
8	24.804896504479657	34.943114402510936	20.447920910885948	19.804068182123462
9	26.687426962116568	14.696901178904195	19.532960513649407	39.08271134532983
10	15.485150642733375	27.685667336786917	32.9864826746305	23.84269934584921
11	34.9536363351437	23.453387838436843	23.373913666423395	18.21906215999606
12	23.61950005149031	25.25398154408242	29.836171270198754	21.29034713422852
13	29.289254644201968	21.922558575822837	25.510313732689184	23.277873047286015
14	22.646109347505856	20.722834384780363	27.610446712007985	29.020609555705796
15	24.727661041537008	27.809244077495155	24.50916303622679	22.95393184474105
16	22.879382832683362	27.69171185127808	25.257115736781543	24.171789579257016
17	22.85901058013907	26.603699242868593	25.777839466649947	24.75945071034239
18	24.116269594301144	25.77515301576499	27.216657786454018	22.89191960347985
19	25.815673649946497	25.420989240764207	26.716977921851143	22.046359187438156
20	25.48792664198117	25.050482889546572	26.671308256806796	22.790282211665467
21	23.975678664654815	26.292294811120115	26.860926915103672	22.871099609121394
22	23.568681355583116	25.99275553744689	26.909730772847144	23.52883233412285
23	23.478013638215657	26.050961973287723	27.55559833977335	22.915426048723265
24	23.568009742861875	26.199164513774775	26.44094509342133	23.79188064994202
25	23.773970977375605	26.295652874726315	27.368666132361437	22.56171001553664
26	24.03298961686733	26.16983742494728	26.74137985072288	23.055793107462513
27	23.944336737663594	26.12730195260205	26.645115360678417	23.283245949055935
28	23.33966141764013	26.128197436230373	27.04517267163063	23.486968474498866
29	24.228428918748293	26.223342571739433	26.15461420326583	23.393614306246448
30	23.98664833910174	25.986263281141564	27.161361672405228	22.86572670735147
31	24.124105076048945	25.65202401687091	26.773841132249498	23.450029774830643
32	22.71931513412106	25.96387619043355	27.317847436454244	23.99896123899115
33	23.104373094298904	25.818583971738533	27.77991698866765	23.297125945294905
34	23.120715670515754	25.71560335448167	27.26479003147625	23.898890943526325
35	23.13526727947596	26.389007042978736	27.004428166542045	23.471297511003257
36	23.833072896844765	25.965219415876028	26.846151435236386	23.355556252042824
37	23.78046323368093	25.84074719153947	26.925177865435675	23.453611709343924
38	23.71263034883565	26.161106459571155	26.494674111120563	23.631589080472637
39	23.359362057463184	25.713812387225033	27.11390104010423	23.812924515207552
40	23.261082729255005	26.369530274062765	27.324563563666647	23.044823433015583
41	23.597112960782294	25.48747890016701	27.13830296897597	23.777105170074726
42	23.041241498502306	26.479674760346196	27.02077074275889	23.458312998392607
43	23.562412970184873	25.624040153485893	27.0230094518297	23.790537424499536
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	17.0
2	27.0
3	125.5
4	224.0
5	224.0
6	260.5
7	297.0
8	316.0
9	335.0
10	428.0
11	521.0
12	521.0
13	852.5
14	1184.0
15	1925.5
16	2667.0
17	3047.5
18	3428.0
19	3428.0
20	3026.5
21	2625.0
22	2742.0
23	2859.0
24	3729.5
25	4600.0
26	4600.0
27	5856.5
28	7113.0
29	9036.5
30	10960.0
31	12700.5
32	14441.0
33	14441.0
34	17267.5
35	20094.0
36	21411.5
37	22729.0
38	25312.0
39	27895.0
40	27895.0
41	29908.0
42	31921.0
43	33127.0
44	34333.0
45	35268.5
46	36204.0
47	36204.0
48	36246.0
49	36288.0
50	36279.0
51	36270.0
52	35334.0
53	34398.0
54	34398.0
55	32090.0
56	29782.0
57	26766.5
58	23751.0
59	21860.5
60	19970.0
61	19970.0
62	17375.0
63	14780.0
64	12009.5
65	9239.0
66	7966.5
67	6694.0
68	6694.0
69	5547.0
70	4400.0
71	3591.0
72	2782.0
73	2301.0
74	1820.0
75	1820.0
76	1357.5
77	895.0
78	679.5
79	464.0
80	389.0
81	314.0
82	314.0
83	236.5
84	159.0
85	138.5
86	118.0
87	89.5
88	61.0
89	61.0
90	46.0
91	31.0
92	18.5
93	6.0
94	3.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	446686.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.24942112731576
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.00090790319831	54.15756154525007
2	7.242112179647443	9.016345818442728
3	2.0653331613402366	3.8569738118543633
4	0.996621136923089	2.4815635542683836
5	0.5865216689645607	1.8255317185835511
6	0.3575676443476239	1.3355027324698503
7	0.273648873538192	1.1924138778926143
8	0.19663460626540902	0.9792312330895496
9	0.15316373150454024	0.8580918261471522
>10	0.9719724835974781	11.568425023116733
>50	0.09497751049736963	4.131385409823722
>100	0.05763902352048549	6.879474304225589
>500	0.0021750574913390747	0.8992555632860647
>1k	7.250191637796917E-4	0.8182435815496287
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2099	0.46990503396121663	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1527	0.3418508751113758	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	812	0.18178317654907475	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	713	0.15961995674814075	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	691	0.15469479679237763	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	678	0.1517844750003358	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	587	0.13141222245604295	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	504	0.11283093716839122	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	491	0.10992061537634938	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	4.4774181416028263E-4	0.0	0.0
2	2.2387090708014131E-4	0.0	4.4774181416028263E-4	0.0	0.0
3	2.2387090708014131E-4	0.0	4.4774181416028263E-4	0.0	0.0
4	2.2387090708014131E-4	0.0	8.954836283205653E-4	0.0	0.0
5	6.71612721240424E-4	0.0	0.0011193545354007065	0.0	0.0
6	8.954836283205653E-4	0.0	0.0024625799778815543	0.0	0.0
7	8.954836283205653E-4	0.0	0.007387739933644663	0.0	0.0
8	8.954836283205653E-4	0.0	0.009402578097365935	0.0	0.0
9	0.001343225442480848	0.0	0.01052193263276664	2.2387090708014131E-4	0.0
10	0.001343225442480848	0.0	0.01208902898232763	2.2387090708014131E-4	0.0
11	0.001343225442480848	0.0	0.016566447123930458	4.4774181416028263E-4	0.0
12	0.001343225442480848	0.0	0.01880515619473187	6.71612721240424E-4	0.0
13	0.001343225442480848	0.0	0.020596123451373	6.71612721240424E-4	0.0
14	0.001343225442480848	0.0	0.022610961615094273	6.71612721240424E-4	0.0
15	0.001343225442480848	0.0	0.023058703429254555	6.71612721240424E-4	0.0
16	0.001343225442480848	0.0	0.025073541592975826	6.71612721240424E-4	0.0
17	0.001343225442480848	0.0	0.02552128340713611	6.71612721240424E-4	0.0
18	0.001343225442480848	0.0	0.02574515431421625	6.71612721240424E-4	0.0
19	0.001343225442480848	0.0	0.026416767035456673	6.71612721240424E-4	0.0
20	0.001343225442480848	0.0	0.026864508849616955	0.0011193545354007065	0.0
21	0.001343225442480848	0.0	0.027312250663777238	0.0011193545354007065	0.0
22	0.001343225442480848	0.0	0.027312250663777238	0.0015670963495609891	0.0
23	0.001343225442480848	0.0	0.027312250663777238	0.002238709070801413	0.0
24	0.001343225442480848	0.0	0.027536121570857382	0.002686450884961696	0.0
25	0.001343225442480848	0.0	0.027536121570857382	0.002910321792041837	0.0
26	0.001343225442480848	0.0	0.027536121570857382	0.0031341926991219782	0.0
27	0.001343225442480848	0.0	0.027536121570857382	0.0038058054203624022	0.0
28	0.001343225442480848	0.0	0.027536121570857382	0.014327738053129044	0.0
29	0.001343225442480848	0.0	0.027536121570857382	0.0335806360620212	0.0
30	0.001343225442480848	0.0	0.027536121570857382	0.06380320851784027	0.0
31	0.0017909672566411305	0.0	0.027536121570857382	0.1499935077436947	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATAAC	45	1.3214431E-4	24.666668	3
GGTATCA	435	0.0	24.666666	1
CGTTACT	40	0.0019293493	23.125	17
GTATCAA	1160	0.0	22.646551	1
AACGTCA	115	5.456968E-12	22.52174	28
TTATACC	50	2.6985194E-4	22.2	4
GCCGTCC	90	9.44583E-8	20.555557	27
CGCCGTC	100	1.2845703E-8	20.35	26
ACGGGTT	55	5.136331E-4	20.181818	16
CTGCGGG	130	3.274181E-11	19.923077	20
AACGGCC	65	6.890063E-5	19.923077	37
GGCGAGT	115	3.0358933E-9	19.304348	19
TGCGGGT	125	4.0927262E-10	19.240002	21
GCGGGTA	125	4.0927262E-10	19.240002	22
TCTGCGG	130	6.9303496E-10	18.5	19
ACTGATC	70	1.21708064E-4	18.5	8
AAGGTCG	50	0.007028314	18.5	35
CCGCTCT	110	3.835885E-8	18.5	28
CGAGGGT	110	3.835885E-8	18.5	16
TTAACGG	70	1.21708064E-4	18.5	35
>>END_MODULE