FastQCFastQC Report
Fri 10 Feb 2017
ERR1632798.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632798.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334430
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT40361.2068295308435248No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29510.8823969141524385No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16000.47842597853063423No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG12290.3674909547588434No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA11570.3459617857249649No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10260.3067906587327692No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT9330.2789821487306761No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG8260.24698741141643993No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA8080.24160511915797028No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7930.23711987560924558No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC7680.22964446969470442No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC7060.21110546302664235No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG6450.19286547259516193No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA6420.191968423885417No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT6190.1850910504440391No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA6070.18150285560505935No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA6050.18090482313189607No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG5740.17163531979786503No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC5650.1689441736686302No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5630.16834614119546693No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG5430.162365816463834No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA5260.157282540441946No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC5130.1533953293663846No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC5090.15219926442005802No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC5070.1516012319468947No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT4970.14861106958107825No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA4960.1483120533444966No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT4900.14651795592500674No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC4860.14532189097868015No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4650.13904255001046556No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT4600.13754746882755733No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4560.13635140388123074No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT4490.13425829022515923No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG4390.13126812785934278No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA4360.1303710791495978No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4340.12977304667643452No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4120.12319468947163831No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT4090.12229764076189338No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4040.12080255957898514No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC3990.1193074783960769No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC3980.11900846215949527No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG3960.11841042968633196No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA3930.11751338097658703No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT3840.11482223484735221No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG3570.10674879645964776No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG3550.10615076398648446No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTAC200.001840070237.03
GACCGTT352.3832834E-531.7142857
TTCAATA250.00549084129.59999818
AATGCGA250.00549084129.59999820
AACGCCC250.00549084129.59999820
GGTATCA5800.027.4310341
AGTCCAA358.856195E-426.4285711
AGCGACG358.856195E-426.4285729
GCTAAAC358.856195E-426.428573
GTATCAA18350.025.8092651
GTCACGA601.3326735E-624.66666819
TACTATA601.3326735E-624.6666685
GGACCGT901.4188117E-1024.6666666
GCCATTG551.897433E-523.54545415
TCCGCCG1750.023.25714131
ACCGAAG400.0019283423.12500215
GTGCTAG400.0019283423.1250021
CACCGAA400.0019283423.12500214
CAATCAA400.0019283423.12500224
ACGTCAC652.6734106E-622.7692317
Produced by FastQC (version 0.11.5)