##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632798.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	334430
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.54119546691385	33.0	33.0	33.0	27.0	33.0
2	31.093266154352182	33.0	33.0	33.0	27.0	33.0
3	31.549612773973628	33.0	33.0	33.0	27.0	33.0
4	31.678811709475823	33.0	33.0	33.0	27.0	33.0
5	31.801492091020542	33.0	33.0	33.0	33.0	33.0
6	34.5742038692701	37.0	37.0	37.0	27.0	37.0
7	34.776524235265974	37.0	37.0	37.0	27.0	37.0
8	34.960212899560446	37.0	37.0	37.0	27.0	37.0
9	35.05935472296146	37.0	37.0	37.0	27.0	37.0
10	35.08119486888138	37.0	37.0	37.0	33.0	37.0
11	35.11737583350776	37.0	37.0	37.0	33.0	37.0
12	35.05523427922136	37.0	37.0	37.0	33.0	37.0
13	35.078354214633855	37.0	37.0	37.0	33.0	37.0
14	35.042609813712886	37.0	37.0	37.0	27.0	37.0
15	35.067090273001824	37.0	37.0	37.0	27.0	37.0
16	35.07918547977155	37.0	37.0	37.0	33.0	37.0
17	35.05606255419669	37.0	37.0	37.0	33.0	37.0
18	35.06093053852824	37.0	37.0	37.0	33.0	37.0
19	35.04590497264002	37.0	37.0	37.0	27.0	37.0
20	35.04862004006817	37.0	37.0	37.0	27.0	37.0
21	35.0362138564124	37.0	37.0	37.0	27.0	37.0
22	35.012355350895554	37.0	37.0	37.0	27.0	37.0
23	35.016529617558234	37.0	37.0	37.0	27.0	37.0
24	35.016819663307714	37.0	37.0	37.0	27.0	37.0
25	35.0077415303651	37.0	37.0	37.0	27.0	37.0
26	34.955380797177284	37.0	37.0	37.0	27.0	37.0
27	34.87371348264211	37.0	37.0	37.0	27.0	37.0
28	34.84110576204288	37.0	37.0	37.0	27.0	37.0
29	34.88349430374069	37.0	37.0	37.0	27.0	37.0
30	34.853266752384656	37.0	37.0	37.0	27.0	37.0
31	34.829982358042045	37.0	37.0	37.0	27.0	37.0
32	34.854172771581496	37.0	37.0	37.0	27.0	37.0
33	34.785264479861254	37.0	37.0	37.0	27.0	37.0
34	34.732419340370186	37.0	37.0	37.0	27.0	37.0
35	34.71673892892384	37.0	37.0	37.0	27.0	37.0
36	34.65940256555931	37.0	37.0	37.0	27.0	37.0
37	34.65711509134946	37.0	37.0	37.0	27.0	37.0
38	34.501246897706544	37.0	37.0	37.0	27.0	37.0
39	34.550937415901686	37.0	37.0	37.0	27.0	37.0
40	34.54812367311545	37.0	37.0	37.0	27.0	37.0
41	34.53437789671979	37.0	37.0	37.0	27.0	37.0
42	34.40741560266722	37.0	37.0	37.0	27.0	37.0
43	34.12415453159107	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	9.0
16	19.0
17	25.0
18	20.0
19	20.0
20	40.0
21	108.0
22	288.0
23	595.0
24	1198.0
25	2193.0
26	3457.0
27	5041.0
28	6914.0
29	9362.0
30	12081.0
31	14937.0
32	18927.0
33	23785.0
34	32749.0
35	56691.0
36	145971.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.8892144843465	18.08330592351165	13.728134437699968	23.299345154441887
2	16.956911760308586	24.169482402894477	37.247854558502524	21.625751278294413
3	20.033489818497145	27.000269114612923	30.160571719044345	22.80566934784559
4	12.991657446999373	18.278862542236045	38.50043357354304	30.22904643722154
5	15.537481685255509	35.58293215321592	32.32933648297103	16.550249678557545
6	27.905391262745567	39.670783123523606	17.227820470651555	15.196005143079269
7	26.246748198427174	33.248512394223006	20.72631043865682	19.778428968693
8	25.282719851687947	33.11634721765392	20.088209789791588	21.512723140866548
9	28.022605627485575	13.82112848727686	18.275274347397065	39.8809915378405
10	16.43871662231259	26.80321741470562	31.037885357174893	25.720180605806895
11	35.78237598301588	23.762820321143437	22.016864515743205	18.43793918009748
12	24.275932183117543	24.93077774123135	29.131656848966898	21.66163322668421
13	31.606315222916603	21.62006996979936	24.73671620368986	22.036898603594175
14	23.983793319977277	21.03340011362617	28.206799629219866	26.77600693717669
15	26.519151989953055	26.07331878120982	25.122746165116766	22.284783063720358
16	21.66043716173788	27.231408665490537	25.55751577310648	25.550638399665104
17	23.12741081840744	25.337140806745808	25.000747540591455	26.534700834255297
18	25.467511885895405	22.68097957719104	27.491253775079986	24.360254761833566
19	27.84020572317077	23.86149567921538	27.496337051101875	20.801961546511976
20	27.762760517896123	22.227371946296685	27.104027748706756	22.90583978710044
21	24.757946356487157	24.801602727028076	27.231408665490537	23.20904225099423
22	24.0794785156834	24.43231767484974	27.457165924109677	24.031037885357176
23	24.047184762132584	24.99117902102084	27.960410250276592	23.001225966569987
24	24.2603833388153	24.17187453278713	27.944562389737765	23.623179738659807
25	24.250814819244685	24.810274197888944	28.870017641957958	22.06889334090841
26	24.788446012618483	24.42932751248393	27.325598780013756	23.45662769488383
27	24.906258409831654	24.515444188619444	27.991806955117664	22.58649044643124
28	23.620189576293992	24.9837036151063	27.1342283886015	24.261878419998204
29	24.200281075262385	25.10181502855605	26.42047663188111	24.277427264300453
30	24.673922794007712	24.955297072631044	27.984331549203123	22.38644858415812
31	24.768112908530934	23.819633406093953	27.271476841192477	24.14077684418264
32	22.509045241156596	24.65837394970547	28.14520228448405	24.687378524653887
33	23.299046138205306	24.480160272702808	28.496845378704062	23.723948210387825
34	22.539245881051343	24.880842029722213	27.533415064438	25.046497024788444
35	23.315791047453878	25.276141494483152	27.638668779714738	23.76939867834823
36	23.562180426397152	24.624585114971744	28.269892055138595	23.54334240349251
37	25.203480548993813	24.73342702508746	26.993092724934964	23.06999970098376
38	24.09951260353437	25.071315372424724	26.63128307867117	24.197888945369733
39	23.769099662111653	24.08814998654427	27.587237987022696	24.555512364321384
40	23.705708219956342	25.12334419758993	27.877283736506893	23.293663845946835
41	24.369225248931016	24.30583380677571	27.470322638519274	23.854618305774004
42	23.62886104715486	25.09045241156595	27.819274586610053	23.46141195466914
43	23.536764046287715	25.327273270938612	27.18296803516431	23.952994647609366
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8.0
1	28.0
2	48.0
3	191.5
4	335.0
5	335.0
6	458.5
7	582.0
8	585.0
9	588.0
10	778.5
11	969.0
12	969.0
13	1492.5
14	2016.0
15	3531.0
16	5046.0
17	5442.5
18	5839.0
19	5839.0
20	4641.0
21	3443.0
22	2741.5
23	2040.0
24	2393.5
25	2747.0
26	2747.0
27	3233.0
28	3719.0
29	4687.0
30	5655.0
31	6217.0
32	6779.0
33	6779.0
34	8109.0
35	9439.0
36	9600.0
37	9761.0
38	11312.5
39	12864.0
40	12864.0
41	15355.5
42	17847.0
43	18933.5
44	20020.0
45	23379.0
46	26738.0
47	26738.0
48	29083.0
49	31428.0
50	32247.0
51	33066.0
52	34032.5
53	34999.0
54	34999.0
55	30903.5
56	26808.0
57	24677.0
58	22546.0
59	19919.0
60	17292.0
61	17292.0
62	14708.0
63	12124.0
64	9230.5
65	6337.0
66	5777.5
67	5218.0
68	5218.0
69	4277.0
70	3336.0
71	2717.0
72	2098.0
73	1794.0
74	1490.0
75	1490.0
76	1008.0
77	526.0
78	402.0
79	278.0
80	219.0
81	160.0
82	160.0
83	122.0
84	84.0
85	89.0
86	94.0
87	69.0
88	44.0
89	44.0
90	30.0
91	16.0
92	9.0
93	2.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	334430.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.843459534654585
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.46361997381875	39.17948003954857
2	7.315319432337616	6.707191007588838
3	2.1856588053185186	3.00594482994543
4	1.0348774416369435	1.8976944847604031
5	0.6801661532380817	1.5590584761405835
6	0.4786083909335566	1.3164638641625188
7	0.3488571263499172	1.1194972288638818
8	0.2415093493057859	0.8857299265712444
9	0.20880252687435957	0.8615007172348498
>10	1.647899207552225	15.469958208000337
>50	0.2244139964730199	7.04542666333111
>100	0.1539584394407927	13.69937427486239
>500	0.012394959107521447	3.664172148702469
>1k	0.00391419761290151	3.588508130287375
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4036	1.2068295308435248	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2951	0.8823969141524385	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1600	0.47842597853063423	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1229	0.3674909547588434	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1157	0.3459617857249649	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1026	0.3067906587327692	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	933	0.2789821487306761	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	826	0.24698741141643993	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	808	0.24160511915797028	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	793	0.23711987560924558	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	768	0.22964446969470442	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	706	0.21110546302664235	No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG	645	0.19286547259516193	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	642	0.191968423885417	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	619	0.1850910504440391	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	607	0.18150285560505935	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	605	0.18090482313189607	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	574	0.17163531979786503	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	565	0.1689441736686302	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	563	0.16834614119546693	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	543	0.162365816463834	No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA	526	0.157282540441946	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	513	0.1533953293663846	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	509	0.15219926442005802	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	507	0.1516012319468947	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	497	0.14861106958107825	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	496	0.1483120533444966	No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT	490	0.14651795592500674	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	486	0.14532189097868015	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	465	0.13904255001046556	No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT	460	0.13754746882755733	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	456	0.13635140388123074	No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT	449	0.13425829022515923	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	439	0.13126812785934278	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	436	0.1303710791495978	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	434	0.12977304667643452	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	412	0.12319468947163831	No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT	409	0.12229764076189338	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	404	0.12080255957898514	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	399	0.1193074783960769	No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC	398	0.11900846215949527	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	396	0.11841042968633196	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	393	0.11751338097658703	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	384	0.11482223484735221	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	357	0.10674879645964776	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	355	0.10615076398648446	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	5.980324731632928E-4	0.0	0.0
2	0.0	0.0	5.980324731632928E-4	0.0	0.0
3	0.0	0.0	0.0014950811829082318	0.0	0.0
4	0.0	0.0	0.0017940974194898783	0.0	0.0
5	0.0	0.0	0.0029901623658164637	0.0	0.0
6	0.0	0.0	0.005681308495051282	0.0	0.0
7	0.0	0.0	0.015249828065663966	0.0	0.0
8	0.0	0.0	0.017043925485153842	0.0	0.0
9	0.0	0.0	0.021828185270460185	0.0017940974194898783	0.0
10	0.0014950811829082318	0.0	0.02392129892653171	0.0017940974194898783	0.0
11	0.0014950811829082318	0.0	0.034984899680052625	0.0020931136560715245	0.0
12	0.0017940974194898783	0.0	0.03887211075561403	0.0020931136560715245	0.0
13	0.0017940974194898783	0.0	0.040965224411685555	0.0020931136560715245	0.0
14	0.0020931136560715245	0.0	0.04335735430433873	0.0020931136560715245	0.0
15	0.0020931136560715245	0.0	0.046048500433573544	0.0020931136560715245	0.0
16	0.0020931136560715245	0.0	0.04933767903597165	0.002392129892653171	0.0
17	0.0020931136560715245	0.0	0.04993571150913494	0.0029901623658164637	2.990162365816464E-4
18	0.0020931136560715245	0.0	0.050832760218879884	0.0029901623658164637	2.990162365816464E-4
19	0.0020931136560715245	0.0	0.054121938821278	0.0032891786023981104	2.990162365816464E-4
20	0.0020931136560715245	0.0	0.055617020004186224	0.0035881948389797566	2.990162365816464E-4
21	0.0020931136560715245	0.0	0.05890619860658434	0.003887211075561403	2.990162365816464E-4
22	0.0020931136560715245	0.0	0.05980324731632928	0.005083276021887988	2.990162365816464E-4
23	0.0020931136560715245	0.0	0.06040127978949257	0.005980324731632927	2.990162365816464E-4
24	0.0020931136560715245	0.0	0.06099931226265586	0.006578357204796221	2.990162365816464E-4
25	0.0020931136560715245	0.0	0.06099931226265586	0.0068773734413778665	2.990162365816464E-4
26	0.0020931136560715245	0.0	0.06099931226265586	0.007774422151122806	2.990162365816464E-4
27	0.0020931136560715245	0.0	0.06129832849923751	0.009269503334031037	2.990162365816464E-4
28	0.0020931136560715245	0.0	0.06129832849923751	0.02212720150704183	2.990162365816464E-4
29	0.0020931136560715245	0.0	0.06129832849923751	0.056514068713931165	2.990162365816464E-4
30	0.0020931136560715245	0.0	0.06129832849923751	0.10166552043775977	2.990162365816464E-4
31	0.0020931136560715245	0.0	0.06129832849923751	0.20871333313398918	2.990162365816464E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGTAC	20	0.0018400702	37.0	3
GACCGTT	35	2.3832834E-5	31.714285	7
TTCAATA	25	0.005490841	29.599998	18
AATGCGA	25	0.005490841	29.599998	20
AACGCCC	25	0.005490841	29.599998	20
GGTATCA	580	0.0	27.431034	1
AGTCCAA	35	8.856195E-4	26.42857	11
AGCGACG	35	8.856195E-4	26.42857	29
GCTAAAC	35	8.856195E-4	26.42857	3
GTATCAA	1835	0.0	25.809265	1
GTCACGA	60	1.3326735E-6	24.666668	19
TACTATA	60	1.3326735E-6	24.666668	5
GGACCGT	90	1.4188117E-10	24.666666	6
GCCATTG	55	1.897433E-5	23.545454	15
TCCGCCG	175	0.0	23.257141	31
ACCGAAG	40	0.00192834	23.125002	15
GTGCTAG	40	0.00192834	23.125002	1
CACCGAA	40	0.00192834	23.125002	14
CAATCAA	40	0.00192834	23.125002	24
ACGTCAC	65	2.6734106E-6	22.76923	17
>>END_MODULE