FastQCFastQC Report
Fri 10 Feb 2017
ERR1632797.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632797.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381828
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT74781.9584734487779838No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT56711.4852237132949915No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT27790.7278146181002965No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA20430.53505766994563No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG19640.5143677257822894No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT18590.48686843290696336No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA18230.4774401039211373No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG17320.45360738342918805No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC15030.3936327351582388No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA14830.3883947746105576No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14730.38577579433671705No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA12870.33706276124328227No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG11870.3108729585048765No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA11620.3043255078202751No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC11210.29358768869752877No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC10990.28782593209507945No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT10780.2823260735200142No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT10560.276564316917565No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC9660.2529934944529998No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC9540.2498507181243911No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG9540.2498507181243911No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC9460.24775553390531863No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG9370.2453984516588621No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA9320.24408896152194182No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA9160.2398985930837969No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8830.231255958180123No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC8730.2286369779062824No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC8490.222351425249065No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA8460.22156573116691286No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT8240.2158039745644636No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA8240.2158039745644636No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT8210.2150182804823114No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC8190.21449448442754326No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA8100.2121374021810868No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA7840.20532805346910127No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC7700.20166148108572446No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT7580.19851870475711578No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT7540.19747111264757952No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA7290.1909236619629781No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG7160.18751898760698535No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC7150.1872570895796013No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT7100.185947599442681No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA7040.18437621127837667No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC6890.18044774086761578No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA6660.17442408623778244No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG6550.1715432079365578No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC6520.17075751385440566No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG6430.16840043160794912No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG6270.16421006316980422No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA6250.1636862671150361No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC6200.1623767769781158No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT6170.16159108289596363No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG6140.16080538881381146No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT6070.15897210262212305No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG6050.15844830656735492No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG5910.15478173418397811No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG5850.1532103460196738No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA5760.15085326377321726No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT5690.14901997758152885No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG5460.14299632295169554No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG5360.14037734267785495No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA5350.1401154446504709No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT5340.13985354662308683No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA5330.13959164859570278No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA5200.13618697423971005No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG5150.13487748410278974No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG5130.13435368804802164No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG5130.13435368804802164No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG5110.1338298919932535No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG5100.13356799396586946No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG5050.13225850382894916No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC5000.13094901369202885No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4930.12911572750034045No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC4900.1283300334181883No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA4890.12806813539080422No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC4880.1278062373634202No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4870.12754433933603612No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG4770.12492535906219555No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC4760.12466346103481149No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT4710.12335397089789119No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT4690.12283017484312309No Hit
GGTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAA4690.12283017484312309No Hit
GAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACC4470.11706841824067381No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA4410.11549703007636947No Hit
GCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTAC4400.1152351320489854No Hit
GAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAA4370.11444943796683324No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT4300.11261615177514483No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG4290.11235425374776077No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG4230.11078286558345643No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4210.1102590695286883No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4200.10999717150130424No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC4150.10868768136438396No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTA4060.10633059911792743No Hit
CTTCCTGAGCCTTTCGGCCCAGGCGGCGGACGGCGACTCGCCG4000.1047592109536231No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC4000.1047592109536231No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG3980.10423541489885499No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG3970.10397351687147093No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC3960.10371161884408686No Hit
GTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACA3890.10187833265239846No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC3870.10135453659763036No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA3840.10056884251547817No Hit
GAGCAAAGGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGT3830.10030694448809412No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC3820.10004504646071005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTGC551.8488208E-830.2727289
ATCTCGT250.00549180329.637
TATGCTT250.00549180329.65
GTATCTA250.00549180329.624
GATTCCT250.00549180329.633
GATATCA453.9990446E-628.7777791
GGTATCA10250.028.5170731
TAGTGCA604.307003E-827.75000210
TTATCAA358.8585046E-426.4285722
TTATAGG358.8585046E-426.4285724
TTTATAG358.8585046E-426.4285723
ACTAGTG1050.026.428578
GTATCAA31750.025.521261
GTGCAGG451.3209517E-424.66666815
ACACGAA451.3209517E-424.66666817
GGTCGGA451.3209517E-424.66666820
CGTGCAG451.3209517E-424.66666814
CACTGTG451.3209517E-424.6666687
CGACATC451.3209517E-424.66666824
CTAGTGG451.3209517E-424.6666689
Produced by FastQC (version 0.11.5)