FastQCFastQC Report
Fri 10 Feb 2017
ERR1632796.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632796.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences385751
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10440.27064090566194254No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7340.1902781846320554No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC4390.11380398236167891No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2100.029.952381
ATCGCTA250.005491872329.610
TGCACCG400.001928874623.1255
GTATCAA5850.023.085471
TACCTTG502.697594E-422.25
GTATAAC603.7180318E-521.5833321
TATTAGA1301.8189894E-1221.3461532
TAGCACA450.003821270520.5555554
TTAACGG1001.2831151E-820.3535
GTATAAA656.886947E-519.9230771
GCACCGC1153.0322553E-919.30434810
ATTAGAG1355.4569682E-1119.1851863
GAAGCTC500.007026616518.517
GTATTAG1700.018.51
AACGGCC1103.831883E-818.537
TAACGGC1156.372284E-817.69565236
GTATTAT752.0627835E-417.2666661
CTTTATA752.0627835E-417.2666662
CCCGAAA752.0627835E-417.26666637
TTTAACG1301.3846147E-817.07692334
Produced by FastQC (version 0.11.5)