##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632796.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	385751
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.664288621416407	33.0	33.0	33.0	27.0	33.0
2	31.182428561429525	33.0	33.0	33.0	27.0	33.0
3	31.636187592514343	33.0	33.0	33.0	27.0	33.0
4	31.7749506806204	33.0	33.0	33.0	27.0	33.0
5	31.88024399159043	33.0	33.0	33.0	33.0	33.0
6	34.73862932306073	37.0	37.0	37.0	27.0	37.0
7	34.920653478539265	37.0	37.0	37.0	27.0	37.0
8	35.077547433448004	37.0	37.0	37.0	33.0	37.0
9	35.163833664721544	37.0	37.0	37.0	33.0	37.0
10	35.203545810639504	37.0	37.0	37.0	33.0	37.0
11	35.24902074135906	37.0	37.0	37.0	33.0	37.0
12	35.179698821260345	37.0	37.0	37.0	33.0	37.0
13	35.21231830896096	37.0	37.0	37.0	33.0	37.0
14	35.17989843189	37.0	37.0	37.0	33.0	37.0
15	35.19386339892832	37.0	37.0	37.0	33.0	37.0
16	35.202625527866424	37.0	37.0	37.0	33.0	37.0
17	35.173562738657836	37.0	37.0	37.0	33.0	37.0
18	35.18068132033358	37.0	37.0	37.0	33.0	37.0
19	35.191698790152195	37.0	37.0	37.0	33.0	37.0
20	35.18833651759814	37.0	37.0	37.0	33.0	37.0
21	35.17877076144974	37.0	37.0	37.0	33.0	37.0
22	35.172847251206086	37.0	37.0	37.0	33.0	37.0
23	35.17757828236349	37.0	37.0	37.0	33.0	37.0
24	35.15207996868446	37.0	37.0	37.0	33.0	37.0
25	35.16099763837294	37.0	37.0	37.0	33.0	37.0
26	35.11403210879557	37.0	37.0	37.0	33.0	37.0
27	35.059030307115215	37.0	37.0	37.0	27.0	37.0
28	35.036842419073444	37.0	37.0	37.0	27.0	37.0
29	35.07659085783316	37.0	37.0	37.0	33.0	37.0
30	35.055722473823785	37.0	37.0	37.0	27.0	37.0
31	35.0287439306703	37.0	37.0	37.0	27.0	37.0
32	35.06268551474915	37.0	37.0	37.0	27.0	37.0
33	35.024927997594304	37.0	37.0	37.0	27.0	37.0
34	34.97663259460118	37.0	37.0	37.0	27.0	37.0
35	34.97687368276427	37.0	37.0	37.0	27.0	37.0
36	34.937625566751606	37.0	37.0	37.0	27.0	37.0
37	34.96963066848822	37.0	37.0	37.0	27.0	37.0
38	34.843505266350576	37.0	37.0	37.0	27.0	37.0
39	34.91821667344997	37.0	37.0	37.0	27.0	37.0
40	34.95879984757006	37.0	37.0	37.0	27.0	37.0
41	34.969651407254936	37.0	37.0	37.0	27.0	37.0
42	34.882429339133274	37.0	37.0	37.0	27.0	37.0
43	34.67278633107885	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	11.0
16	23.0
17	23.0
18	25.0
19	13.0
20	37.0
21	77.0
22	266.0
23	558.0
24	1184.0
25	2108.0
26	3335.0
27	5015.0
28	7006.0
29	9509.0
30	12371.0
31	15556.0
32	19826.0
33	25670.0
34	36462.0
35	64787.0
36	181888.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.243444605457924	20.137601717169883	13.197114200611276	25.421839476760915
2	16.334111901200515	22.718282000565132	37.3227807575353	23.62482534069905
3	17.85530043992109	26.541992114083957	30.021957169262038	25.580750276732918
4	12.905734528231944	17.800601942703974	38.76879126690533	30.52487226215875
5	14.092510453634599	36.74494686987202	35.37929908153187	13.783243594961517
6	30.205236020126975	39.46483612485775	17.23728519174286	13.092642663272422
7	27.080163110400235	31.860708073342646	23.459174441543897	17.59995437471322
8	24.878224554181326	35.7080085340025	20.40902032658373	19.004746585232443
9	26.210949550357615	15.173259434194597	19.924770123732667	38.69102089171512
10	15.001646139608194	28.07147615949149	33.913586743780314	23.01329095712001
11	34.68480963108326	23.312188432434393	23.80628954947622	18.196712387006126
12	23.160017731645542	25.404211525051135	30.134724213287846	21.301046530015476
13	28.270568319978434	21.863067108056754	25.79513727767394	24.071227294290875
14	22.189702683855646	20.518935790185896	27.25177640498664	30.039585120971818
15	24.062413318435986	28.304787285062126	24.222879525911793	23.409919870590095
16	23.37103468299499	28.030257860640674	24.984251499023983	23.614455957340358
17	22.85593556465181	27.169858276452945	26.157028756892398	23.817177402002844
18	23.431695575643356	27.058387405346974	27.24503630580348	22.264880713206185
19	24.890149345043824	26.064482010416047	26.77789558549427	22.267473059045862
20	24.626767007732965	26.03933625577121	26.77426630131873	22.559630435177098
21	23.49935580205884	26.849703565253236	26.81833618059318	22.832604452094746
22	23.236751168499886	26.547954509515204	26.81911388434508	23.396180437639824
23	23.282635689862115	26.460073985550263	27.38631915406571	22.870971170521916
24	23.336038014159392	26.982172437660562	26.062667368328274	23.61912217985177
25	23.66267358995829	26.48651591311494	27.065905208282025	22.784905288644747
26	23.52009456877623	26.916845322500784	26.639464317655694	22.923595791067296
27	23.429362464387648	26.87277544322633	26.281202122612772	23.41665996977325
28	23.157166151221904	26.522549520286404	27.11904829799534	23.201236030496357
29	24.10933477813408	26.381785141192115	26.39526533955842	23.11361474111538
30	23.832472242456923	26.374267338257063	26.954434337176053	22.83882608210996
31	23.66863598538954	26.354047040707606	26.76752620213557	23.20979077176728
32	22.722948223076543	26.396561512478257	27.119307532579306	23.761182731865894
33	22.835456032518387	26.4613701584701	27.37102431361163	23.332149495399882
34	23.541611039245524	26.055149565393222	27.190337808586367	23.21290158677489
35	23.135390446168643	26.97569157306138	26.809262970154325	23.079655010615657
36	23.948090866906373	26.48314586352336	26.403820080829345	23.164943188740924
37	23.449323527353137	26.108033420522563	26.793968129700247	23.64867492242405
38	23.57168225098574	26.487552851450808	26.611466982587213	23.32929791497624
39	23.199680622992553	26.354565509875545	27.09053249375893	23.355221373372977
40	23.226641019725157	26.694940518624712	27.236222329948596	22.842196131701538
41	23.37699707842624	25.91645906297067	27.151193386407297	23.555350472195794
42	22.969739547013486	26.975950807645344	26.92514082918774	23.129168816153424
43	23.550425015100416	25.741994187960625	27.15767425100648	23.54990654593248
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	10.0
2	14.0
3	69.0
4	124.0
5	124.0
6	143.0
7	162.0
8	154.5
9	147.0
10	208.0
11	269.0
12	269.0
13	398.5
14	528.0
15	956.5
16	1385.0
17	1600.0
18	1815.0
19	1815.0
20	1695.5
21	1576.0
22	1910.5
23	2245.0
24	3075.0
25	3905.0
26	3905.0
27	5223.5
28	6542.0
29	8341.0
30	10140.0
31	12379.0
32	14618.0
33	14618.0
34	16994.0
35	19370.0
36	21539.0
37	23708.0
38	25970.5
39	28233.0
40	28233.0
41	29507.5
42	30782.0
43	31327.5
44	31873.0
45	31507.0
46	31141.0
47	31141.0
48	30381.5
49	29622.0
50	29174.5
51	28727.0
52	27208.0
53	25689.0
54	25689.0
55	24510.0
56	23331.0
57	20761.5
58	18192.0
59	16882.5
60	15573.0
61	15573.0
62	13777.0
63	11981.0
64	10013.5
65	8046.0
66	6919.0
67	5792.0
68	5792.0
69	4818.5
70	3845.0
71	3212.5
72	2580.0
73	2086.5
74	1593.0
75	1593.0
76	1236.0
77	879.0
78	701.5
79	524.0
80	437.5
81	351.0
82	351.0
83	262.5
84	174.0
85	176.0
86	178.0
87	118.5
88	59.0
89	59.0
90	42.5
91	26.0
92	15.5
93	5.0
94	3.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	385751.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.73134386835547
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.15051014238637	61.274343686356694
2	6.220436126981713	8.550778689094429
3	1.7062812848536384	3.5182501717624435
4	0.791771012920553	2.1767794301615453
5	0.43785983480932167	1.5047347436210408
6	0.30378587826708375	1.2527766996915184
7	0.20424719187407936	0.982672878519033
8	0.14725638840138677	0.8096903574422261
9	0.11473528252214368	0.7097319141176135
>10	0.8060598097547894	10.667991996519739
>50	0.07829140171937629	3.681333269943769
>100	0.03800553481328434	4.406472073243223
>500	3.8005534813284343E-4	0.1917333867900009
>1k	3.8005534813284343E-4	0.27271070273673154
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1044	0.27064090566194254	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	734	0.1902781846320554	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	439	0.11380398236167891	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	5.184691679347559E-4	0.0	0.0
2	0.0	0.0	5.184691679347559E-4	0.0	0.0
3	0.0	0.0	5.184691679347559E-4	0.0	0.0
4	0.0	0.0	5.184691679347559E-4	7.777037519021338E-4	0.0
5	0.0	2.5923458396737793E-4	5.184691679347559E-4	0.0010369383358695117	0.0
6	0.0	2.5923458396737793E-4	0.0012961729198368895	0.0010369383358695117	0.0
7	0.0	2.5923458396737793E-4	0.0044069879274454245	0.0010369383358695117	0.0
8	0.0	2.5923458396737793E-4	0.004925457095380181	0.0010369383358695117	0.0
9	0.0	2.5923458396737793E-4	0.005443926263314937	0.0012961729198368895	0.0
10	0.0	2.5923458396737793E-4	0.00622163001521707	0.0015554075038042676	0.0
11	0.0	2.5923458396737793E-4	0.009073210438858227	0.0015554075038042676	0.0
12	0.0	2.5923458396737793E-4	0.01011014877472774	0.0015554075038042676	0.0
13	0.0	2.5923458396737793E-4	0.011665556278532007	0.0015554075038042676	0.0
14	0.0	2.5923458396737793E-4	0.01244326003043414	0.0015554075038042676	0.0
15	0.0	2.5923458396737793E-4	0.014776371286140542	0.0015554075038042676	0.0
16	0.0	2.5923458396737793E-4	0.015813309622010052	0.0015554075038042676	0.0
17	0.0	2.5923458396737793E-4	0.016850247957879566	0.0018146420877716454	0.0
18	0.0	2.5923458396737793E-4	0.016850247957879566	0.0018146420877716454	0.0
19	0.0	2.5923458396737793E-4	0.01710948254184694	0.0018146420877716454	0.0
20	0.0	2.5923458396737793E-4	0.018405655461683833	0.0018146420877716454	0.0
21	0.0	2.5923458396737793E-4	0.019442593797553344	0.0018146420877716454	0.0
22	0.0	2.5923458396737793E-4	0.0199610629654881	0.0020738766717390235	0.0
23	0.0	2.5923458396737793E-4	0.0199610629654881	0.0023331112557064015	0.0
24	0.0	2.5923458396737793E-4	0.02022029754945548	0.002851580423641157	0.0
25	0.0	2.5923458396737793E-4	0.02022029754945548	0.002851580423641157	0.0
26	0.0	2.5923458396737793E-4	0.02022029754945548	0.003110815007608535	0.0
27	0.0	2.5923458396737793E-4	0.02022029754945548	0.003888518759510669	0.0
28	0.0	2.5923458396737793E-4	0.02022029754945548	0.01011014877472774	0.0
29	0.0	2.5923458396737793E-4	0.020479532133422854	0.024108816308966146	0.0
30	0.0	2.5923458396737793E-4	0.020479532133422854	0.04743992886603016	0.0
31	0.0	2.5923458396737793E-4	0.020479532133422854	0.11250780944184202	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	210	0.0	29.95238	1
ATCGCTA	25	0.0054918723	29.6	10
TGCACCG	40	0.0019288746	23.125	5
GTATCAA	585	0.0	23.08547	1
TACCTTG	50	2.697594E-4	22.2	5
GTATAAC	60	3.7180318E-5	21.583332	1
TATTAGA	130	1.8189894E-12	21.346153	2
TAGCACA	45	0.0038212705	20.555555	4
TTAACGG	100	1.2831151E-8	20.35	35
GTATAAA	65	6.886947E-5	19.923077	1
GCACCGC	115	3.0322553E-9	19.304348	10
ATTAGAG	135	5.4569682E-11	19.185186	3
GAAGCTC	50	0.0070266165	18.5	17
GTATTAG	170	0.0	18.5	1
AACGGCC	110	3.831883E-8	18.5	37
TAACGGC	115	6.372284E-8	17.695652	36
GTATTAT	75	2.0627835E-4	17.266666	1
CTTTATA	75	2.0627835E-4	17.266666	2
CCCGAAA	75	2.0627835E-4	17.266666	37
TTTAACG	130	1.3846147E-8	17.076923	34
>>END_MODULE