FastQCFastQC Report
Fri 10 Feb 2017
ERR1632795.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632795.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286447
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT50851.7751975059958736No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT40041.3978153026563378No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18660.6514294092799017No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG17740.6193117749531326No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA17450.6091877380457816No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14340.5006161698324647No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT12610.44022105310930115No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA12380.432191644527609No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC10420.36376711922275323No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10270.3585305484086061No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT10160.3546903964782316No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC9560.3337441132216431No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA9190.3208272385467469No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG8710.3040702119414761No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG8410.29359707031318183No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA7960.2778873578707405No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC7900.27579272954508166No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA7860.27439631066130904No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC7810.27265078705659335No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT7710.26915973984716196No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT7670.2677633209633894No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG7460.2604321218235834No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC7340.25624286517226574No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA7310.2551955510094363No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT7040.2457697235439715No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC6890.2405331527298244No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA6700.23390016303190467No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT6600.23040911582247325No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC6520.22761627805492815No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6400.22342702140361043No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA6350.22168149779889473No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC6310.22028507891512214No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC6220.2171431364266339No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC6090.21260477505437309No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA6080.2122556703334299No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG5990.20911372784494167No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT5950.20771730896116908No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT5890.20562268063551026No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG5710.1993387956585337No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5600.19549864372815912No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC5260.18362908321609234No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG5230.1825817690532629No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT5130.17909072184383149No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG5110.1783925124019452No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG5110.1783925124019452No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG4910.17141041798308237No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG4910.17141041798308237No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4840.1689666849364804No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4770.1665229518898784No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA4750.16582474244799214No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC4690.1637301141223333No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC4640.16198459051761757No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA4570.15954085747101557No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG4550.1588426480291293No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA4520.15779533386629988No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC4520.15779533386629988No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT4520.15779533386629988No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG4480.1563989149825273No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC4430.1546533913778116No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT4400.15360607721498218No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA4380.1529078677730959No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG4380.1529078677730959No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG4340.15151144888932333No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA4320.15081323944743705No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC4300.15011503000555076No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG4280.14941682056366448No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG4180.14592577335423307No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG4120.14383114502857422No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA4080.14243472614480165No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT4060.14173651670291537No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG4000.13964188837725652No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG3960.13824546949348396No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA3920.1368490506097114No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT3870.13510352700499567No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG3830.1337071081212231No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC3660.12777232786518972No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA3640.12707411842330343No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC3540.12358307121387202No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT3540.12358307121387202No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT3540.12358307121387202No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3530.12323396649292888No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTAT3520.12288486177198575No Hit
GTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATT3440.1200920240044406No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG3400.11869560512066804No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG3400.11869560512066804No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG3370.11764829095783863No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG3340.11660097679500919No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG3290.11485545319029349No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC3280.11450634846935034No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA3270.11415724374840722No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA3250.11345903430652092No Hit
GCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTAC3250.11345903430652092No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG3210.11206261542274837No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGAC3210.11206261542274837No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC3200.11171351070180521No Hit
GGTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAA3190.11136440598086207No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC3140.10961888237614636No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG3120.1089206729342601No Hit
GAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAA3040.10612783516671495No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA3040.10612783516671495No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG3030.10577873044577182No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC3000.10473141628294239No Hit
GAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACC3000.10473141628294239No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA2980.10403320684105612No Hit
GTATCAACGCAGAGTACGGGGGTTAAGCGACTAAGCGTACACG2920.10193857851539727No Hit
GAGCAAAGGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGT2870.10019305491068156No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTATA352.3820965E-531.7142872
GGACTTT250.0054895429.66
TCCCTAT250.0054895429.61
ACTAGTG250.0054895429.68
ATTTTGA250.0054895429.616
CTAGTGC250.0054895429.69
AGTGAGT250.0054895429.68
GTCGTAT250.0054895429.613
TAGGACT250.0054895429.64
AGTCGTA250.0054895429.612
CTAGTCC453.994457E-628.7777779
GGTATCA8200.027.9756091
AGATCTT405.9248065E-527.758
GTATTAT405.9248065E-527.751
TTATAGA405.9248065E-527.754
TAGATCT405.9248065E-527.757
TATAGAT405.9248065E-527.755
GATCTTG405.9248065E-527.759
GAATTTC405.9248065E-527.7522
CCGCTCT2300.026.54347828
Produced by FastQC (version 0.11.5)