FastQCFastQC Report
Fri 10 Feb 2017
ERR1632794.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632794.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214992
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT48062.2354320160750167No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT41291.920536578105232No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18630.866543871399866No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG11040.5135074793480687No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10830.5037396740343827No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8930.4153642926248418No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT8320.38699114385651556No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG8270.3846654759246856No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7850.3651298652973134No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6950.323267842524373No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC6770.3148954379697849No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA6030.2804755525787006No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5700.26512614422862246No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5610.2609399419513284No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC4740.22047331993748603No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC4610.21442658331472797No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC4490.20884498027833592No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT4420.2055890451737739No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA4340.20186797648284585No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4330.20140284289647986No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA4150.1930304383418918No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA3990.18558830096003573No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC3880.18047183151000967No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC3750.17442509488725164No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3720.1730296941281536No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC3670.17070402619632358No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT3600.16744809109176156No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG3590.16698295750539555No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3560.16558755674629752No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC3550.16512242315993153No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3430.15954082012353948No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC3390.15768028577807547No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3310.15395921708714744No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3310.15395921708714744No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT3290.15302894991441543No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT3260.1516335491553174No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT3250.15116841556895141No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3250.15116841556895141No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA3230.1502381483962194No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG3220.1497730148098534No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3170.14744734687802336No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT3040.14140061025526532No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG3040.14140061025526532No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC3020.1404703430825333No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC3020.1404703430825333No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA3010.1400052094961673No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT3000.1395400759098013No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG3000.1395400759098013No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC2980.1386098087370693No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG2950.1372144079779713No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2920.13581900721887327No Hit
TATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCGG2870.13349333928704324No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG2840.13209793852794524No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT2800.1302374041824812No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG2790.1297722705961152No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG2790.1297722705961152No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC2740.1274466026642852No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG2740.1274466026642852No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2730.12698146907791918No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG2690.12512093473245517No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA2670.12419066755972315No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT2650.12326040038699115No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2620.12186499962789313No Hit
CTTCCTGAGCCTTTCGGCCCAGGCGGCGGACGGCGACTCGCCG2480.11535312941876906No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA2410.11209719431420705No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG2410.11209719431420705No Hit
GAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACC2360.10977152638237701No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT2350.10930639279601102No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA2340.10884125920964502No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT2320.10791099203691301No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG2290.10651559127781499No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT2280.10605045769144898No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC2250.10465505693235098No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG2250.10465505693235098No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA2190.10186425541415495No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG2190.10186425541415495No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT2170.10093398824142293No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG2170.10093398824142293No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCAA452.5993359E-937.01
GGTATCA6100.030.6311471
TTGTTAC250.00548653129.59999815
AGTGCAA358.8458584E-426.4285711
CTAGTGC358.8458584E-426.428579
TAGTGCA358.8458584E-426.4285710
GTTGGTT358.8458584E-426.4285734
CTTAGGA358.8458584E-426.428572
TTATACA659.317773E-825.6153852
TATACAC659.317773E-825.6153853
GCCGCTC2450.024.16326727
CGCTCTC2400.023.89583229
GTATCAA16750.023.7462671
GGCAGCT2650.023.7358518
CCGCTCT2450.023.40816528
GCTCTCT2450.023.40816530
AGCTTCG2700.023.29629721
GCAGCTT2750.022.87272619
TAGGACC652.6660327E-622.769234
GCCGGCA2700.022.61111315
Produced by FastQC (version 0.11.5)