FastQCFastQC Report
Fri 10 Feb 2017
ERR1632793.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632793.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382188
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT47751.2493851193653385No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT37000.9681099354244507No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17860.4673092823427214No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG11220.29357279663411723No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10430.2729023412561357No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA10190.2666227092425717No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG9190.2404575758527217No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8750.2289449171611877No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT8500.22240363381372516No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC7980.20879776445100318No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG7500.19623850042387517No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC7470.19545354642217966No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG7170.18760400640522465No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG6800.17792290705098016No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA6770.17713795304928465No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG6560.17164327503741614No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA6540.17111997236961912No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC6500.17007336703402515No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC6430.16824180769673563No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC6070.15882235967638964No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT5980.15646749767130314No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT5830.15254272766282564No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG5800.15175777366113014No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG5730.14992621432384062No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT5320.1391985096340021No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG5220.1365819962950171No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA5180.13553539095942313No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC5110.13370383162213362No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA5040.1318722722848441No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5000.1308256669492501No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC4900.12820915361026508No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA4880.12768585094246812No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC4820.1261159429390771No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT4810.12585429160517858No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC4670.1221911729305996No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG4660.1219295215967011No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT4650.1216678702628026No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4640.12140621892890409No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG4600.1203596135933101No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4580.1198363109255131No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA4540.1187897055899191No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4520.1182664029221221No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG4510.1180047515882236No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG4500.1177431002543251No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC4440.11617319225093409No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG4400.1151265869153401No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC4240.11094016557296409No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4130.1080620009000806No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT4100.10727704689838509No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4100.10727704689838509No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC4030.10544548756109559No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC4000.10466053355940008No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA3990.10439888222550159No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3940.10309062555600909No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT3920.10256732288821209No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3890.10178236888651658No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC3880.10152071755261807No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT3850.10073576355092259No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT3840.1004741122170241No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCCC308.297466E-637.030
CGGCTTA250.00549181129.59999828
ACTAGTG556.243372E-726.909098
TCACGCC358.858519E-426.42857229
CCCTCAT358.858519E-426.42857234
CTAGTGC358.858519E-426.4285729
GGTATCA8250.026.2363641
GCCGGCA2750.024.89090715
GTATGTA451.3209553E-424.6666661
GCAGGCT451.3209553E-424.66666632
GCCGCTC2650.024.43396227
TTCGCCG2850.024.01754424
CGCTCTC2650.023.7358529
GTTTCCA551.8983537E-523.54545413
GGCAGCT2850.023.3684218
CCGCTCT2700.023.29629728
GCTCTCT2700.023.29629730
TGCTCGC3100.023.27419510
TGATCAC400.001928841723.12526
ATCACGC400.001928841723.12528