##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632793.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	382188
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.726383350602323	33.0	33.0	33.0	27.0	33.0
2	31.232738861502717	33.0	33.0	33.0	27.0	33.0
3	31.66794875820277	33.0	33.0	33.0	27.0	33.0
4	31.788891854270673	33.0	33.0	33.0	27.0	33.0
5	31.921023161376077	33.0	33.0	33.0	33.0	33.0
6	34.72246643013386	37.0	37.0	37.0	27.0	37.0
7	34.87546181460433	37.0	37.0	37.0	27.0	37.0
8	35.08370749474081	37.0	37.0	37.0	33.0	37.0
9	35.148625807194364	37.0	37.0	37.0	33.0	37.0
10	35.17764032361037	37.0	37.0	37.0	33.0	37.0
11	35.23409159889897	37.0	37.0	37.0	33.0	37.0
12	35.11577286571007	37.0	37.0	37.0	33.0	37.0
13	35.18688446523701	37.0	37.0	37.0	33.0	37.0
14	35.1310637696631	37.0	37.0	37.0	33.0	37.0
15	35.19127497461982	37.0	37.0	37.0	33.0	37.0
16	35.183634755669985	37.0	37.0	37.0	33.0	37.0
17	35.16524066689692	37.0	37.0	37.0	33.0	37.0
18	35.150002093210674	37.0	37.0	37.0	33.0	37.0
19	35.15682595999874	37.0	37.0	37.0	33.0	37.0
20	35.08741771065549	37.0	37.0	37.0	33.0	37.0
21	35.15367567793861	37.0	37.0	37.0	33.0	37.0
22	35.148065873339824	37.0	37.0	37.0	33.0	37.0
23	35.14845835034067	37.0	37.0	37.0	33.0	37.0
24	35.121798695929755	37.0	37.0	37.0	33.0	37.0
25	35.13786408783112	37.0	37.0	37.0	33.0	37.0
26	35.11222225710907	37.0	37.0	37.0	33.0	37.0
27	35.02744984143929	37.0	37.0	37.0	27.0	37.0
28	35.00754340795629	37.0	37.0	37.0	27.0	37.0
29	35.036720148199315	37.0	37.0	37.0	27.0	37.0
30	34.98958889342418	37.0	37.0	37.0	27.0	37.0
31	34.95953823772594	37.0	37.0	37.0	27.0	37.0
32	34.97678106062985	37.0	37.0	37.0	27.0	37.0
33	34.93327890985588	37.0	37.0	37.0	27.0	37.0
34	34.860955864653	37.0	37.0	37.0	27.0	37.0
35	34.85052120945713	37.0	37.0	37.0	27.0	37.0
36	34.800831004636464	37.0	37.0	37.0	27.0	37.0
37	34.803703936282666	37.0	37.0	37.0	27.0	37.0
38	34.61972641736528	37.0	37.0	37.0	27.0	37.0
39	34.66642856395282	37.0	37.0	37.0	27.0	37.0
40	34.66296168377866	37.0	37.0	37.0	27.0	37.0
41	34.662836091138395	37.0	37.0	37.0	27.0	37.0
42	34.46021853119407	37.0	37.0	37.0	27.0	37.0
43	34.25175044742378	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	13.0
16	32.0
17	23.0
18	14.0
19	18.0
20	28.0
21	82.0
22	267.0
23	528.0
24	1189.0
25	2113.0
26	3441.0
27	5183.0
28	7317.0
29	9927.0
30	12804.0
31	16430.0
32	20632.0
33	26545.0
34	37031.0
35	65450.0
36	173120.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.63428469758339	19.511078317477264	13.823824923859462	23.030812061079885
2	16.69754152406669	23.582373072937926	38.2937193213811	21.426366081614283
3	17.916836740033702	29.092488513506442	30.10010780034957	22.89056694611029
4	11.429976870022083	18.430719959810354	41.13028143217474	29.009021737992818
5	13.614503856740662	37.011104482610655	34.52358525123761	14.850806409411074
6	26.36451170628068	42.66094173548097	16.658817126649712	14.315729431588641
7	25.86554261253624	33.033742556019554	22.886380524767915	18.214334306676292
8	24.40997624205888	34.27946455671031	19.835002668843607	21.475556532387202
9	27.5652296775409	13.649303484149161	19.491192816100977	39.29427402220897
10	16.588694569164915	26.536939935319793	32.559368687661575	24.314996807853724
11	34.66304541220551	24.49291971490471	22.547803698703255	18.296231174186524
12	24.016190984541637	27.083529571833758	27.767486158644438	21.132793284980167
13	29.261515275204875	22.654034140266045	25.575894585910596	22.508555998618483
14	23.156666352685065	21.06083916815808	28.80676525688928	26.975729222267574
15	26.077218541660123	26.686604498309734	26.38073408898239	20.855442871047757
16	21.534428082514363	27.75440359194951	26.059426250955024	24.651742074581094
17	22.324353459553937	25.65648319675134	25.47829863836646	26.540864705328264
18	24.005463279851803	24.674767391964163	28.625702533831515	22.69406679435252
19	26.997184631647254	23.927752833683947	28.69294692664343	20.38211560802537
20	25.970726448763436	23.632871780380334	28.7332412320638	21.66316053879243
21	22.890305294776393	26.38021078631459	28.84156488429778	21.88791903461124
22	22.911499052822172	25.65569824274964	28.67803280061122	22.75476990381697
23	22.645137994913497	25.96523177075157	29.928987827979945	21.46064240635499
24	22.90443446680691	26.34907427758067	27.495368771389995	23.251122484222424
25	23.080787465854502	26.063874323631303	30.03992799355291	20.815410216961286
26	23.03002710707819	26.297528964802662	28.793159387526558	21.87928454059259
27	23.21318304080714	25.961568652076988	28.43548201408731	22.38976629302856
28	22.080494416360537	25.799606476393816	29.521335049766083	22.598564057479564
29	24.33200414455713	25.66930411211236	27.648173150386725	22.350518592943786
30	24.08029556134677	25.141815022972985	29.258898761865886	21.518990653814356
31	23.130239567961315	25.50210890975122	28.749986917433308	22.617664604854156
32	20.86172250306132	25.390122138842663	29.632536866672947	24.115618491423067
33	21.212858593153108	25.538740096497015	30.34501344887856	22.903387861471316
34	22.181491831245356	25.293834447968017	29.8327001371053	22.691973583681328
35	22.305776214847143	26.495599024563827	28.769872418809587	22.428752341779436
36	23.238301568861395	26.116204590411	28.376348812626244	22.269145028101352
37	22.887950432771305	25.45945974232577	28.679079405946812	22.973510418956117
38	22.73566935644238	26.043465519587215	27.835777156791945	23.385087967178457
39	21.855212617873924	25.707243555527647	28.914042303787664	23.523501522810765
40	22.032612222257107	27.10158351387275	29.39417250149141	21.471631762378724
41	22.89920144012894	25.278135367934105	28.482579254189037	23.340083937747917
42	21.456717636346507	27.36035668309837	28.607910243126423	22.5750154374287
43	23.008048395030716	25.498184139742747	28.321925335175358	23.17184213005118
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	21.0
1	44.5
2	68.0
3	288.5
4	509.0
5	509.0
6	632.5
7	756.0
8	749.0
9	742.0
10	1013.0
11	1284.0
12	1284.0
13	2001.0
14	2718.0
15	4503.5
16	6289.0
17	6540.5
18	6792.0
19	6792.0
20	5071.0
21	3350.0
22	2353.0
23	1356.0
24	1462.5
25	1569.0
26	1569.0
27	2062.0
28	2555.0
29	3586.5
30	4618.0
31	6763.0
32	8908.0
33	8908.0
34	12080.5
35	15253.0
36	17067.0
37	18881.0
38	21525.5
39	24170.0
40	24170.0
41	27258.5
42	30347.0
43	32352.0
44	34357.0
45	34902.0
46	35447.0
47	35447.0
48	35551.0
49	35655.0
50	35721.5
51	35788.0
52	33879.0
53	31970.0
54	31970.0
55	29206.0
56	26442.0
57	21848.0
58	17254.0
59	15781.0
60	14308.0
61	14308.0
62	11726.0
63	9144.0
64	6537.0
65	3930.0
66	3679.0
67	3428.0
68	3428.0
69	2666.0
70	1904.0
71	1488.0
72	1072.0
73	1023.0
74	974.0
75	974.0
76	586.5
77	199.0
78	127.0
79	55.0
80	44.0
81	33.0
82	33.0
83	26.0
84	19.0
85	15.0
86	11.0
87	10.5
88	10.0
89	10.0
90	6.0
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	382188.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.984792824473818
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.9284584419693	17.731587595633563
2	8.87887680408435	4.259160413199786
3	3.908713059225239	2.8124901880749786
4	2.3105370525925357	2.216710100788094
5	1.6047214373765915	1.9244455608234692
6	1.211995592743299	1.7441677917674023
7	0.8901785811687957	1.4945524192282333
8	0.7330882433154788	1.4066375710383372
9	0.5530888978585532	1.1939150365788564
>10	4.782528063534315	23.39817053387338
>50	0.658906694884746	11.036714915172638
>100	0.5072708826513358	23.301097888997038
>500	0.025090817851571447	3.9624478005588872
>1k	0.006545430743888204	3.5179021842653353
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4775	1.2493851193653385	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3700	0.9681099354244507	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1786	0.4673092823427214	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	1122	0.29357279663411723	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1043	0.2729023412561357	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1019	0.2666227092425717	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	919	0.2404575758527217	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	875	0.2289449171611877	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	850	0.22240363381372516	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	798	0.20879776445100318	No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG	750	0.19623850042387517	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	747	0.19545354642217966	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	717	0.18760400640522465	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	680	0.17792290705098016	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	677	0.17713795304928465	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	656	0.17164327503741614	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	654	0.17111997236961912	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	650	0.17007336703402515	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	643	0.16824180769673563	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	607	0.15882235967638964	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	598	0.15646749767130314	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	583	0.15254272766282564	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	580	0.15175777366113014	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	573	0.14992621432384062	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	532	0.1391985096340021	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	522	0.1365819962950171	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	518	0.13553539095942313	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	511	0.13370383162213362	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	504	0.1318722722848441	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	500	0.1308256669492501	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	490	0.12820915361026508	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	488	0.12768585094246812	No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC	482	0.1261159429390771	No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT	481	0.12585429160517858	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	467	0.1221911729305996	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	466	0.1219295215967011	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	465	0.1216678702628026	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	464	0.12140621892890409	No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG	460	0.1203596135933101	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	458	0.1198363109255131	No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA	454	0.1187897055899191	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	452	0.1182664029221221	No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG	451	0.1180047515882236	No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG	450	0.1177431002543251	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	444	0.11617319225093409	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	440	0.1151265869153401	No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC	424	0.11094016557296409	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	413	0.1080620009000806	No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT	410	0.10727704689838509	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	410	0.10727704689838509	No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC	403	0.10544548756109559	No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC	400	0.10466053355940008	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	399	0.10439888222550159	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	394	0.10309062555600909	No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT	392	0.10256732288821209	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	389	0.10178236888651658	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	388	0.10152071755261807	No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT	385	0.10073576355092259	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	384	0.1004741122170241	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0010466053355940009	0.0	0.0
2	2.616513338985002E-4	0.0	0.0015699080033910013	0.0	0.0
3	2.616513338985002E-4	0.0	0.0028781646728835023	0.0	0.0
4	2.616513338985002E-4	0.0	0.003924770008477504	2.616513338985002E-4	0.0
5	5.233026677970004E-4	0.0	0.005756329345767005	2.616513338985002E-4	0.0
6	5.233026677970004E-4	0.0	0.014914126032214512	5.233026677970004E-4	0.0
7	5.233026677970004E-4	0.0	0.02511852805425602	5.233026677970004E-4	0.0
8	5.233026677970004E-4	0.0	0.030351554732226026	5.233026677970004E-4	0.0
9	7.849540016955007E-4	0.0	0.03715448941358703	0.0020932106711880017	0.0
10	0.003401467340680503	0.0	0.04160256208986154	0.002354862005086502	0.0
11	0.003401467340680503	0.0	0.06462787947292956	0.002616513338985002	0.0
12	0.003401467340680503	0.0	0.07430897882717406	0.0028781646728835023	0.0
13	0.003401467340680503	0.0	0.07980365683904257	0.0028781646728835023	0.0
14	0.003401467340680503	0.0	0.08399007818141857	0.0028781646728835023	0.0
15	0.003401467340680503	0.0	0.09393282886956157	0.0028781646728835023	0.0
16	0.003401467340680503	0.0	0.10256732288821209	0.0028781646728835023	0.0
17	0.003401467340680503	0.0	0.10518383622719708	0.0031398160067820026	0.0
18	0.003663118674579003	0.0	0.10858530356787759	0.003401467340680503	0.0
19	0.003663118674579003	0.0	0.11198677090855809	0.003401467340680503	0.0
20	0.003663118674579003	0.0	0.1151265869153401	0.003924770008477504	0.0
21	0.003663118674579003	0.0	0.1182664029221221	0.004709724010173004	0.0
22	0.003663118674579003	0.0	0.1214062189289041	0.005494678011868505	0.0
23	0.003663118674579003	0.0	0.12297612693229511	0.006541283347462505	0.0
24	0.003663118674579003	0.0	0.1240227322678891	0.007326237349158006	0.0
25	0.003663118674579003	0.0	0.1245460349356861	0.007849540016955007	0.0
26	0.003663118674579003	0.0	0.1248076862695846	0.008372842684752007	0.0
27	0.003663118674579003	0.0	0.1248076862695846	0.011251007357635508	0.0
28	0.003663118674579003	0.0	0.12559264027128011	0.029828252064429024	0.0
29	0.003663118674579003	0.0	0.1258542916051786	0.07038420881869656	0.0
30	0.003663118674579003	0.0	0.1258542916051786	0.1297790616136561	0.0
31	0.003663118674579003	0.0	0.1258542916051786	0.2530168398798497	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGCCC	30	8.297466E-6	37.0	30
CGGCTTA	25	0.005491811	29.599998	28
ACTAGTG	55	6.243372E-7	26.90909	8
TCACGCC	35	8.858519E-4	26.428572	29
CCCTCAT	35	8.858519E-4	26.428572	34
CTAGTGC	35	8.858519E-4	26.428572	9
GGTATCA	825	0.0	26.236364	1
GCCGGCA	275	0.0	24.890907	15
GTATGTA	45	1.3209553E-4	24.666666	1
GCAGGCT	45	1.3209553E-4	24.666666	32
GCCGCTC	265	0.0	24.433962	27
TTCGCCG	285	0.0	24.017544	24
CGCTCTC	265	0.0	23.73585	29
GTTTCCA	55	1.8983537E-5	23.545454	13
GGCAGCT	285	0.0	23.36842	18
CCGCTCT	270	0.0	23.296297	28
GCTCTCT	270	0.0	23.296297	30
TGCTCGC	310	0.0	23.274195	10
TGATCAC	40	0.0019288417	23.125	26
ATCACGC	40	0.0019288417	23.125	28
>>END_MODULE