FastQCFastQC Report
Fri 10 Feb 2017
ERR1632792.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632792.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283497
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9010.3178164142830436No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6580.2321012215296811No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3460.12204714688338854No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA2950.10405753852774455No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAATA303.5927005E-430.8333322
GGTATCA2150.027.5348831
GCTAATA451.319779E-424.6666662
CTATGCT551.8961106E-523.5454544
GAAAGCG400.001927618623.12500234
GTATCAA5500.022.5363641
TAATACT909.415271E-820.5555554
CCGTCCG450.003818806720.55555528
TCTACAC450.003818806720.5555553
GGCTAAT450.003818806720.5555551
CGCCGCC555.1299675E-420.18181833
CTGCTCG851.2385481E-619.5882369
ACTGCTC951.665012E-719.4736848
ATACTTG500.007022132618.525
GTTCGCC500.007022132618.524
ACTGTAC500.007022132618.58
TGCTCGG500.007022132618.510
CGCCGTC500.007022132618.526
GGCGAGT609.213434E-418.519
CGTCCGC609.213434E-418.529
Produced by FastQC (version 0.11.5)