##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632792.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	283497
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.06581022021397	33.0	33.0	33.0	27.0	33.0
2	30.76020910274183	33.0	33.0	33.0	27.0	33.0
3	31.193532912164855	33.0	33.0	33.0	27.0	33.0
4	31.36939367965093	33.0	33.0	33.0	27.0	33.0
5	31.497331541427247	33.0	33.0	33.0	27.0	33.0
6	34.11996246873865	37.0	33.0	37.0	27.0	37.0
7	34.328377372600066	37.0	33.0	37.0	27.0	37.0
8	34.49518337054713	37.0	37.0	37.0	27.0	37.0
9	34.592059175229366	37.0	37.0	37.0	27.0	37.0
10	34.653400212347925	37.0	37.0	37.0	27.0	37.0
11	34.654578355326514	37.0	37.0	37.0	27.0	37.0
12	34.63088498290987	37.0	37.0	37.0	27.0	37.0
13	34.653696511779664	37.0	37.0	37.0	27.0	37.0
14	34.63615840732	37.0	37.0	37.0	27.0	37.0
15	34.65716039323168	37.0	37.0	37.0	27.0	37.0
16	34.66448674941886	37.0	37.0	37.0	27.0	37.0
17	34.61652856996723	37.0	37.0	37.0	27.0	37.0
18	34.6371107983506	37.0	37.0	37.0	27.0	37.0
19	34.642997985869336	37.0	37.0	37.0	27.0	37.0
20	34.64280398028903	37.0	37.0	37.0	27.0	37.0
21	34.62678617410413	37.0	37.0	37.0	27.0	37.0
22	34.61880725369228	37.0	37.0	37.0	27.0	37.0
23	34.593113860111394	37.0	37.0	37.0	27.0	37.0
24	34.60057778389189	37.0	37.0	37.0	27.0	37.0
25	34.57504312214944	37.0	37.0	37.0	27.0	37.0
26	34.543868189081365	37.0	37.0	37.0	27.0	37.0
27	34.4834231050064	37.0	37.0	37.0	27.0	37.0
28	34.44066427510697	37.0	37.0	37.0	27.0	37.0
29	34.465602810611756	37.0	37.0	37.0	27.0	37.0
30	34.42975057937121	37.0	37.0	37.0	27.0	37.0
31	34.43340141165515	37.0	37.0	37.0	27.0	37.0
32	34.4458001319238	37.0	37.0	37.0	27.0	37.0
33	34.417623466914996	37.0	37.0	37.0	27.0	37.0
34	34.33479013887272	37.0	37.0	37.0	27.0	37.0
35	34.33711115108802	37.0	37.0	37.0	27.0	37.0
36	34.299650437218034	37.0	37.0	37.0	27.0	37.0
37	34.349019566344616	37.0	37.0	37.0	27.0	37.0
38	34.225247533483596	37.0	37.0	37.0	27.0	37.0
39	34.28942457944881	37.0	37.0	37.0	27.0	37.0
40	34.31722381541957	37.0	37.0	37.0	27.0	37.0
41	34.334109355654554	37.0	37.0	37.0	27.0	37.0
42	34.231021845028344	37.0	37.0	37.0	27.0	37.0
43	33.95854277117571	37.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	12.0
16	17.0
17	22.0
18	19.0
19	21.0
20	48.0
21	151.0
22	379.0
23	781.0
24	1604.0
25	2518.0
26	3935.0
27	5489.0
28	7384.0
29	9391.0
30	11575.0
31	14560.0
32	17834.0
33	22648.0
34	30755.0
35	51897.0
36	102457.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.07486146237879	20.59704335495614	13.229769627191823	25.09832555547325
2	16.326098688875014	23.774149285530356	36.88151902842005	23.01823299717457
3	18.489437277995886	27.010867839871324	30.11918997379161	24.380504908341184
4	13.399436325604858	18.483793479296075	38.406402889624935	29.710367305474133
5	14.458001319237946	36.92702215543727	35.075503444480894	13.539473080843889
6	29.67368261392537	39.740103069873754	17.388543794114224	13.197670522086655
7	26.40839232866662	32.502989449623804	23.332169299851497	17.756448921858077
8	24.38791239413468	36.05576073115412	20.709919328952335	18.84640754575886
9	26.11350384660155	15.199808110844208	20.004797228894837	38.6818908136594
10	14.604740085433003	28.567145331343895	34.12417062614419	22.703943957078913
11	34.23457743820922	23.549102812375438	24.161807708723547	18.05451204069179
12	22.61399591530069	25.56711358497621	30.732247607558456	21.086642892164644
13	28.206647689393538	22.112050568436352	26.439080484096834	23.24222125807328
14	22.083126100099825	20.827733626810865	27.803468819775873	29.285671453313437
15	23.94804883296825	28.73787024201314	24.335001781323964	22.97907914369464
16	22.866555907117185	28.585134939699536	25.161465553427377	23.386843599755906
17	22.427750558206967	27.669428600655387	26.331142833962968	23.57167800717468
18	22.975199032088522	27.154431969297736	27.711051616066484	22.159317382547258
19	24.644352497557293	26.605572545741225	27.209459006620882	21.5406159500806
20	24.658109256888082	26.148424851056625	27.223921240789146	21.96954465126615
21	23.27537857543466	26.953724378035748	27.081415323618945	22.68948172291065
22	22.776255127920226	26.972772198647604	27.087411859737493	23.163560813694676
23	22.856326521973777	26.92479990969922	27.581244245970858	22.637629322356144
24	23.205183829105774	27.009809627615105	26.798167176372235	22.98683936690688
25	23.270792988991065	26.7413764519554	27.685301784498602	22.302528774554933
26	23.321587177289356	26.995347393446846	27.062367503007085	22.620697926256717
27	23.382963488149784	26.97771052250994	26.802752762815835	22.836573226524443
28	22.791422836925964	26.955840802548174	27.333269840597964	22.9194665199279
29	23.35262807013831	27.00769320310268	26.921978010349317	22.7177007164097
30	23.288077122509232	26.917039686486984	27.325509617385723	22.46937357361806
31	23.454569184153623	26.81509857247167	26.95090247868584	22.779429764688867
32	22.56955100053969	26.902930189737457	27.22462671562662	23.302892094096233
33	22.763203843426915	26.76183522224221	27.64685340585615	22.828107528474728
34	22.896538587709923	26.68705488946973	27.229212302070216	23.187194220750133
35	23.0277569074805	27.133620461592184	27.149846382854136	22.68877624807317
36	23.253508855472898	26.739965502280448	27.15831208090386	22.8482135613428
37	23.315590641170804	26.403806742223026	27.136795098360828	23.143807518245342
38	23.46056572027217	26.72232863134354	26.864481811095004	22.95262383728928
39	22.980842830788333	26.72091768166859	27.30363989742396	22.99459959011912
40	22.805532333675487	27.10645968034935	27.52268983446033	22.56531815151483
41	22.975199032088522	26.31033132625742	27.482830506142925	23.231639135511134
42	22.808354233025394	26.759366060311045	27.437680116544445	22.99459959011912
43	23.128639809239605	26.163239822643625	27.55831631375288	23.14980405436389
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	9.0
2	13.0
3	53.5
4	94.0
5	94.0
6	128.5
7	163.0
8	150.0
9	137.0
10	186.0
11	235.0
12	235.0
13	371.5
14	508.0
15	925.0
16	1342.0
17	1518.0
18	1694.0
19	1694.0
20	1678.5
21	1663.0
22	2001.5
23	2340.0
24	3086.5
25	3833.0
26	3833.0
27	4873.5
28	5914.0
29	7263.5
30	8613.0
31	10062.5
32	11512.0
33	11512.0
34	13016.0
35	14520.0
36	15860.0
37	17200.0
38	18447.5
39	19695.0
40	19695.0
41	20753.5
42	21812.0
43	21887.5
44	21963.0
45	22092.0
46	22221.0
47	22221.0
48	21853.0
49	21485.0
50	21251.5
51	21018.0
52	20834.0
53	20650.0
54	20650.0
55	18932.5
56	17215.0
57	15389.5
58	13564.0
59	12113.5
60	10663.0
61	10663.0
62	9355.5
63	8048.0
64	6654.5
65	5261.0
66	4492.0
67	3723.0
68	3723.0
69	3057.5
70	2392.0
71	2041.0
72	1690.0
73	1369.0
74	1048.0
75	1048.0
76	795.0
77	542.0
78	419.5
79	297.0
80	236.5
81	176.0
82	176.0
83	142.0
84	108.0
85	93.0
86	78.0
87	59.0
88	40.0
89	40.0
90	29.0
91	18.0
92	10.0
93	2.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	283497.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.39234748737032
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.34419864179245	64.49884422902856
2	5.289176769552089	7.552134917079789
3	1.4571811591091632	3.120947509895113
4	0.751870847331643	2.1471129919329686
5	0.4133273993845067	1.4754206661454896
6	0.30331953420981955	1.2992816151608846
7	0.21537843645894145	1.0763460523874278
8	0.16707072356943073	0.954205692162815
9	0.12565019571179128	0.807341619070106
>10	0.849368613984175	11.372571363539013
>50	0.06110294347742056	2.9844396629125467
>100	0.021361191622187657	2.158443230954931
>500	9.935437963808215E-4	0.5529104497303217
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	901	0.3178164142830436	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	658	0.2321012215296811	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	346	0.12204714688338854	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	295	0.10405753852774455	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	3.5273741873811715E-4	0.0	0.0
2	0.0	0.0	0.0010582122562143514	0.0	0.0
3	0.0	0.0	0.0014109496749524686	0.0	0.0
4	0.0	0.0	0.0014109496749524686	0.0	0.0
5	0.0	0.0	0.0017636870936905858	0.0	0.0
6	0.0	0.0	0.00246916193116682	0.0	0.0
7	0.0	0.0	0.0052910612810717576	0.0	0.0
8	0.0	0.0	0.006349273537286109	0.0	0.0
9	0.0	0.0	0.006349273537286109	3.5273741873811715E-4	0.0
10	7.054748374762343E-4	0.0	0.006702010956024226	7.054748374762343E-4	0.0
11	7.054748374762343E-4	0.0	0.010934859980881633	7.054748374762343E-4	0.0
12	7.054748374762343E-4	0.0	0.010934859980881633	7.054748374762343E-4	0.0
13	7.054748374762343E-4	0.0	0.0123458096558341	7.054748374762343E-4	0.0
14	7.054748374762343E-4	0.0	0.013404021912048453	7.054748374762343E-4	0.0
15	7.054748374762343E-4	0.0	0.014462234168262804	7.054748374762343E-4	0.0
16	7.054748374762343E-4	0.0	0.01622592126195339	7.054748374762343E-4	0.0
17	7.054748374762343E-4	0.0	0.016578658680691506	7.054748374762343E-4	0.0
18	7.054748374762343E-4	0.0	0.016578658680691506	0.0010582122562143514	0.0
19	7.054748374762343E-4	0.0	0.016931396099429622	0.0010582122562143514	0.0
20	7.054748374762343E-4	0.0	0.017636870936905857	0.0014109496749524686	0.0
21	7.054748374762343E-4	0.0	0.018342345774382093	0.0014109496749524686	0.0
22	7.054748374762343E-4	0.0	0.018342345774382093	0.0014109496749524686	0.0
23	7.054748374762343E-4	0.0	0.018342345774382093	0.0014109496749524686	0.0
24	7.054748374762343E-4	0.0	0.018342345774382093	0.0017636870936905858	0.0
25	7.054748374762343E-4	0.0	0.018342345774382093	0.0017636870936905858	0.0
26	7.054748374762343E-4	0.0	0.018342345774382093	0.0017636870936905858	0.0
27	7.054748374762343E-4	0.0	0.018342345774382093	0.0031746367686430544	0.0
28	7.054748374762343E-4	0.0	0.018342345774382093	0.013051284493310335	0.0
29	7.054748374762343E-4	0.0	0.018342345774382093	0.02574983156788255	0.0
30	7.054748374762343E-4	0.0	0.018342345774382093	0.04479765217974088	0.0
31	7.054748374762343E-4	0.0	0.018342345774382093	0.10229385143405398	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATAATA	30	3.5927005E-4	30.833332	2
GGTATCA	215	0.0	27.534883	1
GCTAATA	45	1.319779E-4	24.666666	2
CTATGCT	55	1.8961106E-5	23.545454	4
GAAAGCG	40	0.0019276186	23.125002	34
GTATCAA	550	0.0	22.536364	1
TAATACT	90	9.415271E-8	20.555555	4
CCGTCCG	45	0.0038188067	20.555555	28
TCTACAC	45	0.0038188067	20.555555	3
GGCTAAT	45	0.0038188067	20.555555	1
CGCCGCC	55	5.1299675E-4	20.181818	33
CTGCTCG	85	1.2385481E-6	19.588236	9
ACTGCTC	95	1.665012E-7	19.473684	8
ATACTTG	50	0.0070221326	18.5	25
GTTCGCC	50	0.0070221326	18.5	24
ACTGTAC	50	0.0070221326	18.5	8
TGCTCGG	50	0.0070221326	18.5	10
CGCCGTC	50	0.0070221326	18.5	26
GGCGAGT	60	9.213434E-4	18.5	19
CGTCCGC	60	9.213434E-4	18.5	29
>>END_MODULE