FastQCFastQC Report
Fri 10 Feb 2017
ERR1632791.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632791.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323012
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT53981.6711453444454076No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT42301.3095488712493653No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19430.6015256399143066No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG12880.3987467957846767No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11940.3696457097569131No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA11850.36685943556276546No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG10680.33063787103884684No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT9430.2919396183423526No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9430.2919396183423526No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG8110.2510742634948547No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7900.24457295704184367No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC7870.24364419897712777No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA6820.21113766671207262No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC6390.1978254677844786No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6190.1916337473530395No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT5890.18234616670588089No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG5680.17584486025286988No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA5470.16934355379985883No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC5460.1690339677782869No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA5370.1662476935841393No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA5350.16562852154099536No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5270.16315183336841974No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5210.16129431723898802No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG5110.15819845702326849No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC5010.15510259680754895No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT4980.15417383874283308No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4910.15200673659182942No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC4900.15169715057025746No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4850.15014922046239768No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA4790.14829170433296596No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA4630.1433383279878147No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT4450.13776577959951952No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC4440.13745619357794756No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4240.1312644731465085No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT4200.13002612906022068No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT4140.12816861293078893No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT4080.12631109680135724No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC4010.12414399465035354No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG3970.12290565056406573No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC3860.11950020432677425No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3830.11857144626205839No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3810.11795227421891447No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC3770.11671393013262664No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT3680.11392765593847907No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3640.11268931185219125No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG3540.1095934516364717No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC3520.1089742795933278No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG3520.1089742795933278No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT3430.10618800539918022No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3400.10525924733446436No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG3380.10464007529132045No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA3350.1037113172266046No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG3320.10278255916188872No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3270.10123462905402894No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGAG352.383033E-531.7142873
TGAGCTA303.5938006E-430.83333410
TTCTAAG358.8555366E-426.4285722
CACTACG358.8555366E-426.4285724
AGGCACT358.8555366E-426.4285721
ACTACGG358.8555366E-426.4285725
GGTATCA8700.026.1551741
AACTGAG451.3203369E-424.6666667
ACTAGTG551.8971736E-523.5454548
GCCGTCC1900.023.36842227
GGCACTA400.001928198423.1250022
GCACTAC400.001928198423.1250023
GAGTCGC2300.022.5217422
GTATCAA21350.022.2693211
CGAGTCG2250.022.221
ACTGAGA502.6962775E-422.28
CCGCTCT2250.022.228
TATTAGA502.6962775E-422.22
CCGGCAG2400.021.58333416
GCCGCTC2350.021.2553227
Produced by FastQC (version 0.11.5)