##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632791.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	323012
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.72844662117816	33.0	33.0	33.0	27.0	33.0
2	31.251170235161542	33.0	33.0	33.0	27.0	33.0
3	31.677340160737064	33.0	33.0	33.0	27.0	33.0
4	31.78993659678278	33.0	33.0	33.0	27.0	33.0
5	31.898489839386773	33.0	33.0	33.0	33.0	33.0
6	34.71658947655195	37.0	37.0	37.0	27.0	37.0
7	34.84960620658056	37.0	37.0	37.0	27.0	37.0
8	35.075761891199086	37.0	37.0	37.0	33.0	37.0
9	35.146694240461656	37.0	37.0	37.0	33.0	37.0
10	35.16380196401372	37.0	37.0	37.0	33.0	37.0
11	35.22537862370438	37.0	37.0	37.0	33.0	37.0
12	35.10364940002229	37.0	37.0	37.0	33.0	37.0
13	35.16268745433606	37.0	37.0	37.0	33.0	37.0
14	35.126942652285365	37.0	37.0	37.0	33.0	37.0
15	35.17677052245737	37.0	37.0	37.0	33.0	37.0
16	35.19041088256783	37.0	37.0	37.0	33.0	37.0
17	35.1701391898753	37.0	37.0	37.0	33.0	37.0
18	35.13908461605141	37.0	37.0	37.0	33.0	37.0
19	35.14696667616064	37.0	37.0	37.0	33.0	37.0
20	35.100999343677636	37.0	37.0	37.0	33.0	37.0
21	35.13947159857838	37.0	37.0	37.0	33.0	37.0
22	35.14542493777321	37.0	37.0	37.0	33.0	37.0
23	35.13097655814644	37.0	37.0	37.0	33.0	37.0
24	35.128199571532946	37.0	37.0	37.0	33.0	37.0
25	35.1308496278776	37.0	37.0	37.0	33.0	37.0
26	35.08601228437334	37.0	37.0	37.0	33.0	37.0
27	35.00019194333338	37.0	37.0	37.0	27.0	37.0
28	34.97943420058697	37.0	37.0	37.0	27.0	37.0
29	34.9936782534395	37.0	37.0	37.0	27.0	37.0
30	34.96498891682043	37.0	37.0	37.0	27.0	37.0
31	34.93153505132936	37.0	37.0	37.0	27.0	37.0
32	34.93387861751266	37.0	37.0	37.0	27.0	37.0
33	34.8863633549218	37.0	37.0	37.0	27.0	37.0
34	34.80412182829121	37.0	37.0	37.0	27.0	37.0
35	34.77734573328545	37.0	37.0	37.0	27.0	37.0
36	34.731718945426174	37.0	37.0	37.0	27.0	37.0
37	34.69576672074103	37.0	37.0	37.0	27.0	37.0
38	34.51620682822929	37.0	37.0	37.0	27.0	37.0
39	34.535596200760345	37.0	37.0	37.0	27.0	37.0
40	34.51772689559521	37.0	37.0	37.0	27.0	37.0
41	34.475678922145306	37.0	37.0	37.0	27.0	37.0
42	34.25969623419564	37.0	37.0	37.0	27.0	37.0
43	34.03190283952299	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	10.0
16	16.0
17	28.0
18	18.0
19	18.0
20	49.0
21	70.0
22	236.0
23	526.0
24	1053.0
25	1749.0
26	3052.0
27	4562.0
28	6615.0
29	8591.0
30	11097.0
31	14032.0
32	17758.0
33	22532.0
34	31376.0
35	54268.0
36	145355.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.18346067638354	18.20892103079762	13.841900610503636	22.76571768231521
2	16.830334476737708	24.090745854643174	37.46424281450844	21.614676854110684
3	19.627134595618738	27.635505801642047	30.078449097866333	22.658910504872885
4	12.155895137022773	18.013881837207286	39.251482917043326	30.57874010872661
5	15.074052976360012	35.94881923891373	32.24245538865429	16.73467239607197
6	27.15162284992508	41.126645449704654	16.422919272349016	15.29881242802125
7	25.852290317387588	33.155424566269986	21.107574950775824	19.884710165566606
8	25.60214481195745	32.53439500699664	19.6209428751873	22.242517305858605
9	28.127747575941452	13.035119438287124	18.641722288955208	40.19541069681622
10	17.12877540153307	26.40056716159152	30.847151189429496	25.623506247445917
11	36.22218369596176	23.641846123363838	21.48279320892103	18.65317697175337
12	24.6975344569242	25.631245897985217	28.173256721112526	21.497962923978058
13	31.51771451215435	21.842841752009214	24.595061483783883	22.044382252052554
14	23.867843919111365	20.77012618726239	28.901712629871334	26.460317263754906
15	26.667120726164974	25.779537602318182	26.131846494867062	21.421495176649785
16	21.18373311208252	27.201466199398165	25.841145220611	25.773655467908313
17	22.694512897353658	24.538716827857787	25.330018698995705	27.43675157579285
18	25.48914591408369	22.279048456404098	28.529280645920274	23.702524983591942
19	27.61445395217515	23.44866444590294	28.48315232870605	20.453729273215856
20	26.987851844513518	22.070697063886172	28.508847968496525	22.432603123103785
21	23.94957462880636	24.86378215050834	28.83298453308236	22.353658687602938
22	23.63689274701869	24.343368048245885	28.771376914789542	23.248362289945884
23	23.433804316867484	24.803103290280237	29.557725409582307	22.205366983269972
24	23.712122150260672	24.378351268683517	28.625561898629154	23.283964682426657
25	23.331021757705596	25.257265983926292	30.237576312954317	21.174135945413795
26	23.94431166643964	24.84644533330031	28.666117667455076	22.543125332804973
27	24.280522085866778	24.518903322477183	29.189627629933252	22.010946961722784
28	23.02917538667294	24.815486731143118	28.596460812601386	23.558877069582554
29	24.14616175250455	24.92105556449915	27.49123871558951	23.441543967406783
30	24.47339417730611	24.45481901601179	29.303864871893303	21.7679219347888
31	23.82264435996186	24.091365026686315	28.693670823374983	23.392319789976842
32	21.272893886295247	24.506519881614306	29.690847398858246	24.5297388332322
33	22.03354674129754	24.519522494520327	30.030153678501108	23.41677708568103
34	21.987728010104888	24.971208499993807	29.201082312731412	23.83998117716989
35	22.53971988656768	25.45292434955977	28.864871893304272	23.142483870568277
36	23.07189825764987	24.848302849429743	29.249377732096644	22.830421160823747
37	23.7879707255458	25.048295419365225	28.30173492006489	22.861998935024086
38	22.943110472675937	25.370264881800058	27.754077247904103	23.932547397619903
39	22.524550171510658	24.37215954825208	28.77818780726413	24.32510247297314
40	22.527646031726377	25.91235000557255	29.182197565415525	22.37780639728555
41	23.444949413644075	24.569675430014986	28.399564102881627	23.585811053459313
42	22.180290515522643	26.10707961314131	28.68840786100826	23.02422201032779
43	22.90688890815202	25.242715440912413	28.226505516822904	23.623890134112667
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4.0
1	39.5
2	75.0
3	298.5
4	522.0
5	522.0
6	727.5
7	933.0
8	900.0
9	867.0
10	1146.0
11	1425.0
12	1425.0
13	2336.0
14	3247.0
15	5267.5
16	7288.0
17	7575.0
18	7862.0
19	7862.0
20	5912.5
21	3963.0
22	2874.0
23	1785.0
24	1814.5
25	1844.0
26	1844.0
27	2165.5
28	2487.0
29	2824.0
30	3161.0
31	4186.0
32	5211.0
33	5211.0
34	6841.0
35	8471.0
36	9225.0
37	9979.0
38	11262.5
39	12546.0
40	12546.0
41	15177.5
42	17809.0
43	19995.0
44	22181.0
45	24580.0
46	26979.0
47	26979.0
48	28942.5
49	30906.0
50	31583.0
51	32260.0
52	32127.5
53	31995.0
54	31995.0
55	28805.0
56	25615.0
57	22780.0
58	19945.0
59	18155.0
60	16365.0
61	16365.0
62	13460.0
63	10555.0
64	7992.0
65	5429.0
66	5119.5
67	4810.0
68	4810.0
69	3883.0
70	2956.0
71	2256.0
72	1556.0
73	1410.5
74	1265.0
75	1265.0
76	807.0
77	349.0
78	241.0
79	133.0
80	114.0
81	95.0
82	95.0
83	73.5
84	52.0
85	50.5
86	49.0
87	37.0
88	25.0
89	25.0
90	17.5
91	10.0
92	6.5
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	323012.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.43927480231618
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.00781450008574	24.980776564940694
2	10.310268744849486	6.6891528219980785
3	3.6144205730044536	3.517475466565097
4	1.9578584829629735	2.5404603741152707
5	1.204815391055371	1.9541668778252603
6	0.8905716682378791	1.7333699446275428
7	0.5893851370378034	1.3383458497339056
8	0.4746983777663482	1.2319096899661013
9	0.3620072571719072	1.0568927606249015
>10	2.8820961702139947	19.10915049118088
>50	0.3879361276438875	8.770882998079127
>100	0.2931959422295705	18.050963762423248
>500	0.01795077197323901	3.7513705829789594
>1k	0.005983590657746337	3.528798751218911
>5k	9.972651096243896E-4	1.7462830637220095
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5398	1.6711453444454076	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4230	1.3095488712493653	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1943	0.6015256399143066	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1288	0.3987467957846767	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1194	0.3696457097569131	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1185	0.36685943556276546	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	1068	0.33063787103884684	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	943	0.2919396183423526	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	943	0.2919396183423526	No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG	811	0.2510742634948547	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	790	0.24457295704184367	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	787	0.24364419897712777	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	682	0.21113766671207262	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	639	0.1978254677844786	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	619	0.1916337473530395	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	589	0.18234616670588089	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	568	0.17584486025286988	No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA	547	0.16934355379985883	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	546	0.1690339677782869	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	537	0.1662476935841393	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	535	0.16562852154099536	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	527	0.16315183336841974	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	521	0.16129431723898802	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	511	0.15819845702326849	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	501	0.15510259680754895	No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT	498	0.15417383874283308	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	491	0.15200673659182942	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	490	0.15169715057025746	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	485	0.15014922046239768	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	479	0.14829170433296596	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	463	0.1433383279878147	No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT	445	0.13776577959951952	No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC	444	0.13745619357794756	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	424	0.1312644731465085	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	420	0.13002612906022068	No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT	414	0.12816861293078893	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	408	0.12631109680135724	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	401	0.12414399465035354	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	397	0.12290565056406573	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	386	0.11950020432677425	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	383	0.11857144626205839	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	381	0.11795227421891447	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	377	0.11671393013262664	No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT	368	0.11392765593847907	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	364	0.11268931185219125	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	354	0.1095934516364717	No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC	352	0.1089742795933278	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	352	0.1089742795933278	No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT	343	0.10618800539918022	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	340	0.10525924733446436	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	338	0.10464007529132045	No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA	335	0.1037113172266046	No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG	332	0.10278255916188872	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	327	0.10123462905402894	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0018575161294317238	0.0	0.0
2	9.287580647158619E-4	0.0	0.0018575161294317238	0.0	0.0
3	9.287580647158619E-4	0.0	0.0034054462372914937	0.0	0.0
4	9.287580647158619E-4	0.0	0.005262962366723218	0.0	0.0
5	0.001238344086287816	0.0	0.007430064517726895	0.0	0.0
6	0.001238344086287816	0.0	0.013312198927594021	0.0	0.0
7	0.0015479301078597698	0.0	0.03343529032977103	0.0	0.0
8	0.0015479301078597698	0.0	0.041794112912213785	0.0	0.0
9	0.0018575161294317238	0.0	0.049533763451512634	3.09586021571954E-4	0.0
10	0.005262962366723218	0.0	0.05696382796923953	6.19172043143908E-4	0.0
11	0.005262962366723218	0.0	0.07832526345770435	0.001238344086287816	0.0
12	0.005262962366723218	0.0	0.08792243012643493	0.001238344086287816	0.0
13	0.005262962366723218	0.0	0.09442373657944597	0.001238344086287816	0.0
14	0.005262962366723218	0.0	0.100925043032457	0.001238344086287816	0.0
15	0.005262962366723218	0.0	0.11423724196005101	0.001238344086287816	0.0
16	0.005262962366723218	0.0	0.1256919247582133	0.0015479301078597698	0.0
17	0.005262962366723218	0.0	0.13033571508179262	0.0015479301078597698	0.0
18	0.005572548388295171	0.0	0.13405074734065608	0.0027862741941475856	0.0
19	0.005572548388295171	0.0	0.13621784949165974	0.0030958602157195396	0.0
20	0.005572548388295171	0.0	0.13838495164266343	0.0030958602157195396	0.0
21	0.005882134409867125	0.0	0.14240956992309883	0.0034054462372914937	0.0
22	0.005882134409867125	0.0	0.1433383279878147	0.004953376345151264	0.0
23	0.005882134409867125	0.0	0.14519584411724643	0.006501306453011033	0.0
24	0.005882134409867125	0.0	0.14674377422510618	0.007739650539298849	0.0
25	0.005882134409867125	0.0	0.14767253228982205	0.008358822582442757	0.0
26	0.006501306453011033	0.0	0.14798211831139402	0.00928758064715862	0.0
27	0.006501306453011033	0.0	0.14860129035453792	0.012073854841306205	0.0
28	0.006501306453011033	0.0	0.14922046239768183	0.03962701076121011	0.0
29	0.006501306453011033	0.0	0.14922046239768183	0.094114150557874	0.0
30	0.006501306453011033	0.0	0.14953004841925377	0.16377100541156367	0.0
31	0.006501306453011033	0.0	0.14953004841925377	0.3145393979171052	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGAG	35	2.383033E-5	31.714287	3
TGAGCTA	30	3.5938006E-4	30.833334	10
TTCTAAG	35	8.8555366E-4	26.428572	2
CACTACG	35	8.8555366E-4	26.428572	4
AGGCACT	35	8.8555366E-4	26.428572	1
ACTACGG	35	8.8555366E-4	26.428572	5
GGTATCA	870	0.0	26.155174	1
AACTGAG	45	1.3203369E-4	24.666666	7
ACTAGTG	55	1.8971736E-5	23.545454	8
GCCGTCC	190	0.0	23.368422	27
GGCACTA	40	0.0019281984	23.125002	2
GCACTAC	40	0.0019281984	23.125002	3
GAGTCGC	230	0.0	22.52174	22
GTATCAA	2135	0.0	22.269321	1
CGAGTCG	225	0.0	22.2	21
ACTGAGA	50	2.6962775E-4	22.2	8
CCGCTCT	225	0.0	22.2	28
TATTAGA	50	2.6962775E-4	22.2	2
CCGGCAG	240	0.0	21.583334	16
GCCGCTC	235	0.0	21.25532	27
>>END_MODULE