FastQCFastQC Report
Fri 10 Feb 2017
ERR1632790.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632790.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414615
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT33850.8164200523377109No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26430.6374588473644224No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG13880.33476839959962856No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA12400.2990726336480831No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT12250.2954548195313725No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11790.2843601895734597No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10720.25855311554092353No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA9100.21948072308044814No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC9060.21851597264932526No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA8070.19463839947903477No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC8010.1931912738323505No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT7360.1775140793266042No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC7260.17510220324879708No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC6900.16641944936869144No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG6820.16448994850644574No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT6810.16424876089866502No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG6800.1640075732908843No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG6780.1635251980753229No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6730.16231926003641933No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA6540.15773669548858582No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA6420.15484244419521725No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC6420.15484244419521725No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG6420.15484244419521725No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC6330.15267175572519084No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA6280.15146581768628728No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC6260.15098344247072584No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG6190.14929512921626087No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC6100.14712444074623446No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG6060.1461596903151116No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT6020.14519493988398877No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA6010.14495375227620805No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT5930.14302425141396236No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG5900.14230068859062023No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG5770.13916524968947097No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT5750.13868287447390953No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC5700.13747693643500597No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT5670.13675337361166384No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA5460.1316884338482689No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5420.13072368341714602No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG5420.13072368341714602No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG5390.1300001205938039No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC5370.12951774537824245No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC5320.1283118073393389No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA5220.1258999312615318No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5180.12493518083040893No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5120.12348805518372465No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT5120.12348805518372465No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT5110.12324686757594396No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT4960.11962905345923326No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA4960.11962905345923326No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA4900.11818192781254898No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4880.11769955259698757No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC4870.11745836498920686No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC4790.11552886412696116No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC4770.11504648891139974No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC4750.1145641136958383No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT4680.11287580044137331No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG4630.11166986240246976No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG4560.10998154914800477No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT4520.10901679871688193No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT4470.10781086067797836No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG4450.10732848546241695No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT4360.10515779699239053No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA4340.1046754217768291No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC4290.10346948373792554No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA4280.10322829613014484No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG4260.1027459209145834No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT4230.10202235809124129No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC4200.10129879526789914No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGAAT200.00184057937.04
GGTATCA7000.031.1857151
TCCCGAA303.5955445E-430.83333422
TTGCTCT250.00549234229.59999816
TCTCGCG250.00549234229.59999820
ACAATCC250.00549234229.59999836
GGACGGT250.00549234229.59999820
TCCGTAC250.00549234229.59999818
CTTTATG250.00549234229.5999981
CTCTCGC250.00549234229.59999819
TCGGCGT405.9311114E-527.75000211
CAAGCAC405.9311114E-527.75000233
ACCAAAT405.9311114E-527.75000222
AAGGGTA358.859795E-426.4285736
GAATCAA509.072799E-625.8999981
TCCCTTG901.4188117E-1024.66666631
AATTCAA451.3212198E-424.66666629
TCATCGG451.3212198E-424.6666668
ATCGGCG451.3212198E-424.66666610
TTCCCTT952.8012437E-1023.36842230
Produced by FastQC (version 0.11.5)