##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632790.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	414615
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.524467276871313	33.0	33.0	33.0	27.0	33.0
2	31.046549208301677	33.0	33.0	33.0	27.0	33.0
3	31.500466698021057	33.0	33.0	33.0	27.0	33.0
4	31.65519096028846	33.0	33.0	33.0	27.0	33.0
5	31.79100611410586	33.0	33.0	33.0	33.0	33.0
6	34.584409633033054	37.0	33.0	37.0	27.0	37.0
7	34.80792542479168	37.0	37.0	37.0	27.0	37.0
8	34.96849366279561	37.0	37.0	37.0	27.0	37.0
9	35.07471509713831	37.0	37.0	37.0	33.0	37.0
10	35.08484256478902	37.0	37.0	37.0	33.0	37.0
11	35.11577246361082	37.0	37.0	37.0	33.0	37.0
12	35.024449187800734	37.0	37.0	37.0	27.0	37.0
13	35.08885833845857	37.0	37.0	37.0	33.0	37.0
14	35.04302304547593	37.0	37.0	37.0	27.0	37.0
15	35.06743123138333	37.0	37.0	37.0	27.0	37.0
16	35.055429736020166	37.0	37.0	37.0	27.0	37.0
17	35.02802117627196	37.0	37.0	37.0	27.0	37.0
18	35.04644790950641	37.0	37.0	37.0	27.0	37.0
19	35.05411526355776	37.0	37.0	37.0	27.0	37.0
20	35.05045403567165	37.0	37.0	37.0	27.0	37.0
21	35.029130639267755	37.0	37.0	37.0	27.0	37.0
22	35.01870168710732	37.0	37.0	37.0	27.0	37.0
23	35.01042895216044	37.0	37.0	37.0	27.0	37.0
24	34.99500259276678	37.0	37.0	37.0	27.0	37.0
25	35.00148330378785	37.0	37.0	37.0	27.0	37.0
26	34.933646877223445	37.0	37.0	37.0	27.0	37.0
27	34.86803178852671	37.0	37.0	37.0	27.0	37.0
28	34.85565645237148	37.0	37.0	37.0	27.0	37.0
29	34.88824813381088	37.0	37.0	37.0	27.0	37.0
30	34.82867961844121	37.0	37.0	37.0	27.0	37.0
31	34.79120871169639	37.0	37.0	37.0	27.0	37.0
32	34.83533398453987	37.0	37.0	37.0	27.0	37.0
33	34.80748646334551	37.0	37.0	37.0	27.0	37.0
34	34.75086043679076	37.0	37.0	37.0	27.0	37.0
35	34.7198605935627	37.0	37.0	37.0	27.0	37.0
36	34.71901161318331	37.0	37.0	37.0	27.0	37.0
37	34.7057438828793	37.0	37.0	37.0	27.0	37.0
38	34.52772330957635	37.0	37.0	37.0	27.0	37.0
39	34.60625399466976	37.0	37.0	37.0	27.0	37.0
40	34.60225269225667	37.0	37.0	37.0	27.0	37.0
41	34.61571578452299	37.0	37.0	37.0	27.0	37.0
42	34.51111995465673	37.0	37.0	37.0	27.0	37.0
43	34.23690411586653	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	11.0
16	26.0
17	30.0
18	14.0
19	20.0
20	42.0
21	109.0
22	272.0
23	728.0
24	1425.0
25	2605.0
26	4225.0
27	6202.0
28	8673.0
29	11495.0
30	14843.0
31	18529.0
32	23456.0
33	29678.0
34	41168.0
35	71448.0
36	179616.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.09395463261097	19.815973855263316	13.277136620720428	23.81293489140528
2	17.31847617669404	23.58670091530697	37.21862450707283	21.87619840092616
3	18.338699757606456	28.36052723611061	29.92607599821521	23.374697008067724
4	11.910326447427131	18.783449706354087	40.91072440697997	28.395499439238815
5	13.762406087575222	37.26372658972782	34.87235145858206	14.101515864114903
6	26.72334575449513	42.21120798813357	17.302316606972735	13.763129650398561
7	26.209133774706654	32.6360599592393	22.9260880575956	18.22871820845845
8	24.308816613002424	34.25249930658563	20.231057728253923	21.207626352158027
9	27.148800694620313	13.768435777769739	19.463357572687915	39.61940595492204
10	16.63350337059682	26.46479263895421	32.7060043654957	24.19569962495327
11	35.20929054665171	24.1103192117989	23.05777649144387	17.622613750105522
12	23.604789985890527	26.556926305126442	28.46013771812404	21.37814599085899
13	29.7936640015436	22.24883325494736	24.80542189742291	23.15208084608613
14	22.829371826875537	21.16855395969755	28.19362541152636	27.80844880190056
15	25.990376614449552	27.070655909699358	25.49280657959794	21.44616089625315
16	21.979426697056308	27.437984636349384	25.778613894818086	24.803974771776225
17	22.72927896964654	25.884012879418254	25.300338868588934	26.086369282346272
18	23.600930984166034	25.080616957900702	28.101250557746344	23.21720150018692
19	26.666425479058887	24.597759367123718	27.655053483352027	21.08076167046537
20	26.008948060248667	24.03338036491685	27.8029014869216	22.154770087912883
21	23.411839899665953	26.545590487560748	27.79976604802045	22.242803564752844
22	23.079001000928574	25.999300555937438	27.4915282852767	23.43017015785729
23	23.473342739650036	26.12327098633672	28.744015532481942	21.6593707415313
24	23.260374081979666	26.57983912786561	25.999782931153	24.160003859001726
25	23.616125803456217	26.63362396440071	28.57132520531095	21.178925026832122
26	23.742749297541092	26.703809558264897	27.404218371260086	22.14922277293393
27	23.52905707704738	26.45828057354413	27.27638893913631	22.736273410272183
28	22.83684864271674	26.188150452829735	28.158412020790376	22.816588883663158
29	24.998854358863042	26.15631368860268	26.069727337409404	22.775104615124874
30	24.62525475441072	25.17178587364181	28.214608733403278	21.98835063854419
31	24.289521604379967	25.29599749164888	27.419654378158047	22.994826525813103
32	21.50091048321937	25.421897422910412	28.60653859604694	24.47065349782328
33	21.770075853502647	25.872435874244783	29.211678303968743	23.145809968283828
34	22.608685165756185	25.62232432497618	28.263087442567205	23.505903066700434
35	22.988796835618587	26.572121124416626	27.51082329389916	22.928258746065627
36	24.08089432364965	26.015942500874306	27.12178768254887	22.78137549292717
37	23.696682464454977	25.523919781001652	27.204032656802095	23.575365097741276
38	23.213101310854647	26.179226511341845	26.793290160751543	23.814382017051962
39	22.598072911013833	25.76076601184231	27.884664085959265	23.75649699118459
40	22.715048900787476	26.9013422090373	28.464479095064092	21.91912979511113
41	23.354919624229705	25.0456447547725	27.77275303594901	23.82668258504878
42	21.88656946806073	27.31449658116566	27.873569456001352	22.925364494772257
43	23.55896434041219	25.3032331198823	27.590656392074575	23.547146147630933
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	16.5
2	31.0
3	170.0
4	309.0
5	309.0
6	419.0
7	529.0
8	520.0
9	511.0
10	676.0
11	841.0
12	841.0
13	1350.0
14	1859.0
15	3145.5
16	4432.0
17	4615.5
18	4799.0
19	4799.0
20	3592.0
21	2385.0
22	1718.5
23	1052.0
24	1250.5
25	1449.0
26	1449.0
27	2051.5
28	2654.0
29	3945.5
30	5237.0
31	7543.5
32	9850.0
33	9850.0
34	13570.5
35	17291.0
36	19076.5
37	20862.0
38	23665.5
39	26469.0
40	26469.0
41	30106.0
42	33743.0
43	36305.0
44	38867.0
45	39120.0
46	39373.0
47	39373.0
48	39805.0
49	40237.0
50	39432.5
51	38628.0
52	37174.0
53	35720.0
54	35720.0
55	32765.5
56	29811.0
57	24654.0
58	19497.0
59	17684.0
60	15871.0
61	15871.0
62	13398.0
63	10925.0
64	7643.0
65	4361.0
66	3860.5
67	3360.0
68	3360.0
69	2664.5
70	1969.0
71	1413.0
72	857.0
73	745.0
74	633.0
75	633.0
76	377.0
77	121.0
78	81.0
79	41.0
80	30.5
81	20.0
82	20.0
83	14.5
84	9.0
85	7.5
86	6.0
87	4.5
88	3.0
89	3.0
90	2.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	414615.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.95723743714048
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.64726817372966	16.170905538873413
2	8.972077593971749	3.9400407607057146
3	3.789626309892572	2.4962917405303715
4	2.3100243854214724	2.0288701566513514
5	1.515850523957029	1.6641944936869144
6	1.154462971506404	1.5209290546651715
7	0.9402667018168238	1.4451961458220277
8	0.6788374085546695	1.1924315328678412
9	0.5613040708275665	1.1092218081834955
>10	5.016586480370834	22.937665062769074
>50	0.7919769766471143	12.225317463188741
>100	0.5689931676882181	24.129855408029137
>500	0.045036138755245064	6.212992776431147
>1k	0.007689096860651596	2.9260880575956008
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3385	0.8164200523377109	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2643	0.6374588473644224	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1388	0.33476839959962856	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1240	0.2990726336480831	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1225	0.2954548195313725	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1179	0.2843601895734597	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1072	0.25855311554092353	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	910	0.21948072308044814	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	906	0.21851597264932526	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	807	0.19463839947903477	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	801	0.1931912738323505	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	736	0.1775140793266042	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	726	0.17510220324879708	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	690	0.16641944936869144	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	682	0.16448994850644574	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	681	0.16424876089866502	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	680	0.1640075732908843	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	678	0.1635251980753229	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	673	0.16231926003641933	No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA	654	0.15773669548858582	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	642	0.15484244419521725	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	642	0.15484244419521725	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	642	0.15484244419521725	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	633	0.15267175572519084	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	628	0.15146581768628728	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	626	0.15098344247072584	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	619	0.14929512921626087	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	610	0.14712444074623446	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	606	0.1461596903151116	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	602	0.14519493988398877	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	601	0.14495375227620805	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	593	0.14302425141396236	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	590	0.14230068859062023	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	577	0.13916524968947097	No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT	575	0.13868287447390953	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	570	0.13747693643500597	No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT	567	0.13675337361166384	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	546	0.1316884338482689	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	542	0.13072368341714602	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	542	0.13072368341714602	No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG	539	0.1300001205938039	No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC	537	0.12951774537824245	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	532	0.1283118073393389	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	522	0.1258999312615318	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	518	0.12493518083040893	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	512	0.12348805518372465	No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT	512	0.12348805518372465	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	511	0.12324686757594396	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	496	0.11962905345923326	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	496	0.11962905345923326	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	490	0.11818192781254898	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	488	0.11769955259698757	No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC	487	0.11745836498920686	No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC	479	0.11552886412696116	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	477	0.11504648891139974	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	475	0.1145641136958383	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	468	0.11287580044137331	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	463	0.11166986240246976	No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG	456	0.10998154914800477	No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT	452	0.10901679871688193	No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT	447	0.10781086067797836	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	445	0.10732848546241695	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT	436	0.10515779699239053	No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA	434	0.1046754217768291	No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC	429	0.10346948373792554	No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA	428	0.10322829613014484	No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG	426	0.1027459209145834	No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT	423	0.10202235809124129	No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC	420	0.10129879526789914	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	9.647504311228489E-4	0.0	0.0
2	0.0	0.0	9.647504311228489E-4	0.0	0.0
3	0.0	0.0	0.0014471256466842735	0.0	0.0
4	0.0	0.0	0.0016883132544649856	0.0	0.0
5	2.4118760778071223E-4	0.0	0.002894251293368547	0.0	0.0
6	2.4118760778071223E-4	0.0	0.0067532530178599426	0.0	0.0
7	4.8237521556142445E-4	0.0	0.012541755604597036	4.8237521556142445E-4	0.0
8	4.8237521556142445E-4	0.0	0.015194819290184871	4.8237521556142445E-4	0.0
9	7.235628233421367E-4	0.0	0.019053821014676266	9.647504311228489E-4	0.0
10	0.0014471256466842735	0.0	0.021948072308044814	0.001205938038903561	0.0
11	0.0014471256466842735	0.0	0.03617814116710683	0.001205938038903561	0.0
12	0.0014471256466842735	0.0	0.038590017244913954	0.001205938038903561	0.0
13	0.0014471256466842735	0.0	0.04220783136162464	0.001205938038903561	0.0
14	0.0014471256466842735	0.0	0.044860895047212475	0.001205938038903561	0.0
15	0.0014471256466842735	0.0	0.048961084379484585	0.001205938038903561	0.0
16	0.0014471256466842735	0.0	0.05378483653509883	0.001205938038903561	0.0
17	0.0016883132544649856	0.0	0.054990774574002386	0.001205938038903561	0.0
18	0.0019295008622456978	0.0	0.05643790022068666	0.0014471256466842735	0.0
19	0.0019295008622456978	0.0	0.058849776298493785	0.0016883132544649856	0.0
20	0.0019295008622456978	0.0	0.06053808955295877	0.0019295008622456978	0.0
21	0.0019295008622456978	0.0	0.06391471606188874	0.002411876077807122	0.0
22	0.0019295008622456978	0.0	0.06536184170857301	0.0026530636855878345	0.0
23	0.0019295008622456978	0.0	0.06656777974747657	0.0038590017244913956	0.0
24	0.0019295008622456978	0.0	0.06656777974747657	0.00434137694005282	0.0
25	0.0019295008622456978	0.0	0.06656777974747657	0.004823752155614244	0.0
26	0.0019295008622456978	0.0	0.06656777974747657	0.0055473149789563815	0.0
27	0.0019295008622456978	0.0	0.06656777974747657	0.006994440625640655	0.0
28	0.0019295008622456978	0.0	0.06680896735525729	0.02387757317029051	0.0
29	0.0019295008622456978	0.0	0.06680896735525729	0.06126165237630091	0.0
30	0.0019295008622456978	0.0	0.06680896735525729	0.1085344235013205	0.0
31	0.0019295008622456978	0.0	0.06680896735525729	0.21972191068822883	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGAAT	20	0.001840579	37.0	4
GGTATCA	700	0.0	31.185715	1
TCCCGAA	30	3.5955445E-4	30.833334	22
TTGCTCT	25	0.005492342	29.599998	16
TCTCGCG	25	0.005492342	29.599998	20
ACAATCC	25	0.005492342	29.599998	36
GGACGGT	25	0.005492342	29.599998	20
TCCGTAC	25	0.005492342	29.599998	18
CTTTATG	25	0.005492342	29.599998	1
CTCTCGC	25	0.005492342	29.599998	19
TCGGCGT	40	5.9311114E-5	27.750002	11
CAAGCAC	40	5.9311114E-5	27.750002	33
ACCAAAT	40	5.9311114E-5	27.750002	22
AAGGGTA	35	8.859795E-4	26.42857	36
GAATCAA	50	9.072799E-6	25.899998	1
TCCCTTG	90	1.4188117E-10	24.666666	31
AATTCAA	45	1.3212198E-4	24.666666	29
TCATCGG	45	1.3212198E-4	24.666666	8
ATCGGCG	45	1.3212198E-4	24.666666	10
TTCCCTT	95	2.8012437E-10	23.368422	30
>>END_MODULE