FastQCFastQC Report
Fri 10 Feb 2017
ERR1632789.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632789.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172023
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC3410.19822930654621765No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3360.19532271847369245No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG3330.19357876563017737No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT3060.17788319003854136No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC2930.17032606104997586No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA2880.1674194729774507No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2570.14939862692779454No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC2520.14649203885526935No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA2440.14184149793922907No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT2260.13137778087813837No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT1980.11510088767199736No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC1910.1110316643704621No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT1890.10986902914145201No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA1890.10986902914145201No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG1850.10754375868343186No Hit
CATCAAAAGTTCCCAAAGAGGGCTTGCTCTCTCTTCACCTGCT1770.10289321776739158No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC1750.10173058253838149No Hit
CTGTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGC1750.10173058253838149No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGAGA200.00183758837.02
GGACCGT200.00183758837.06
GGTATCA1000.033.31
CTATAGT250.005483519329.5999984
CTCTCCT250.005483519329.59999815
AGTACTG250.005483519329.5999985
TAGGTAC358.8386383E-426.428573
ACCATGC400.001924553523.1250028
GGGGCTT450.003812792520.55555536
AAACGTC555.1186973E-420.18181815
GTCACGA555.1186973E-420.18181819
CGTCACG555.1186973E-420.18181818
CAAACGT701.2115659E-418.514
GGTATTC801.6036858E-518.521
ACGTCAC609.193354E-418.517
CCTCTTG701.2115659E-418.515
TTGGCTA500.0070111818.49999822
GTACCTA500.0070111818.4999981
TAAAGCT500.0070111818.49999827
TGGACAG500.0070111818.4999985
Produced by FastQC (version 0.11.5)