##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632789.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	172023
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.48007533876284	33.0	33.0	33.0	14.0	33.0
2	30.384913645268366	33.0	33.0	33.0	27.0	33.0
3	30.839411008992982	33.0	33.0	33.0	27.0	33.0
4	31.008376786825018	33.0	33.0	33.0	27.0	33.0
5	31.13627247519227	33.0	33.0	33.0	27.0	33.0
6	33.3262703243171	37.0	33.0	37.0	27.0	37.0
7	33.58062003336763	37.0	33.0	37.0	27.0	37.0
8	33.68217622062166	37.0	33.0	37.0	27.0	37.0
9	33.73940693976968	37.0	33.0	37.0	27.0	37.0
10	33.89139243008202	37.0	33.0	37.0	27.0	37.0
11	33.834365172099076	37.0	33.0	37.0	27.0	37.0
12	33.89894374589444	37.0	33.0	37.0	27.0	37.0
13	33.897804363370014	37.0	33.0	37.0	27.0	37.0
14	33.895583730082606	37.0	33.0	37.0	27.0	37.0
15	33.94980322398749	37.0	33.0	37.0	27.0	37.0
16	33.908471541596185	37.0	33.0	37.0	27.0	37.0
17	33.9295966237073	37.0	33.0	37.0	27.0	37.0
18	33.901798015381665	37.0	33.0	37.0	27.0	37.0
19	33.90173407044407	37.0	33.0	37.0	27.0	37.0
20	33.90330362800323	37.0	33.0	37.0	27.0	37.0
21	33.9083320253687	37.0	33.0	37.0	27.0	37.0
22	33.94428070664969	37.0	33.0	37.0	27.0	37.0
23	33.897577649500356	37.0	33.0	37.0	27.0	37.0
24	33.87281933229859	37.0	33.0	37.0	27.0	37.0
25	33.8873638990135	37.0	33.0	37.0	27.0	37.0
26	33.808938339640626	37.0	33.0	37.0	27.0	37.0
27	33.71624143283166	37.0	33.0	37.0	27.0	37.0
28	33.683489998430446	37.0	33.0	37.0	27.0	37.0
29	33.74752213366817	37.0	33.0	37.0	27.0	37.0
30	33.694139737128175	37.0	33.0	37.0	27.0	37.0
31	33.681071717154104	37.0	33.0	37.0	27.0	37.0
32	33.69801131244078	37.0	33.0	37.0	27.0	37.0
33	33.67513646431001	37.0	33.0	37.0	27.0	37.0
34	33.561849287595265	37.0	33.0	37.0	27.0	37.0
35	33.580108473866865	37.0	33.0	37.0	27.0	37.0
36	33.53920696651029	37.0	33.0	37.0	27.0	37.0
37	33.6157141777553	37.0	33.0	37.0	27.0	37.0
38	33.49275968911134	37.0	33.0	37.0	27.0	37.0
39	33.56310493364259	37.0	33.0	37.0	27.0	37.0
40	33.58667154973463	37.0	33.0	37.0	27.0	37.0
41	33.60512257081902	37.0	33.0	37.0	27.0	37.0
42	33.4868709416764	37.0	33.0	37.0	27.0	37.0
43	33.20111845509031	37.0	33.0	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	12.0
16	14.0
17	15.0
18	19.0
19	30.0
20	77.0
21	201.0
22	449.0
23	883.0
24	1591.0
25	2523.0
26	3501.0
27	4771.0
28	6111.0
29	7433.0
30	8910.0
31	10898.0
32	12829.0
33	15976.0
34	21198.0
35	32565.0
36	42016.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.671886898844924	21.676171209663824	12.850025868633846	24.80191602285741
2	16.399551222801602	24.98793765949902	35.80044528929271	22.812065828406666
3	18.843991791795283	27.345180586316946	30.856920295541872	22.953907326345895
4	14.492248129610575	19.363689739162787	36.748574318550425	29.395487812676212
5	14.871848531882364	37.91411613563302	34.69245391604611	12.521581416438499
6	30.18433581555955	39.45286386122786	17.23606727007435	13.126733053138242
7	25.701795690111208	32.92001650942025	23.70613231951541	17.672055480953127
8	23.76019485766438	37.34209960296007	20.67165437179912	18.22605116757643
9	25.232091057591138	16.122262720682702	19.943263400824307	38.70238282090185
10	14.234724426384842	29.488498630997018	34.43551153043488	21.841265412183255
11	33.62515477581486	23.73461688262616	24.44091778424978	18.199310557309197
12	21.71744476029368	26.152898158967115	30.711009574301112	21.41864750643809
13	27.167297396278407	22.433628061363887	27.193456688931132	23.205617853426578
14	21.406439836533487	21.502938560541324	27.582939490649505	29.507682112275685
15	23.47011736802637	29.55186225097807	24.49498032239875	22.483040058596814
16	22.70161548165071	29.217604622637673	24.824587409823103	23.256192485888516
17	22.361544677165263	28.55955308301797	26.158711335112166	22.920190904704604
18	22.084837492660867	28.172976869372118	27.788144608569787	21.95404102939723
19	24.06829319335205	27.381222278416256	27.73408207042082	20.816402457810874
20	23.906686896519652	26.506920586200682	28.147398894333897	21.438993622945766
21	22.28887997535213	28.22413281944856	26.708637798433934	22.778349406765376
22	22.17377908768014	27.7433831522529	26.935932985705403	23.14690477436157
23	22.141225301267852	27.931730059352528	27.272515884503818	22.654528754875802
24	23.617772042110648	28.122983554524684	26.239514483528364	22.0197299198363
25	23.47128000325538	27.264377437900745	27.097539282537802	22.166803276306073
26	22.899263470582422	27.686414026031404	26.76967614795696	22.644646355429217
27	22.676037506612488	27.100445870610322	27.058009684751454	23.16550693802573
28	22.03368154258442	27.66781186236724	27.65851078053516	22.639995814513174
29	23.19922335966702	27.99916290263511	26.70747516320492	22.09413857449295
30	22.31271399754684	27.971840974753377	27.151601820686764	22.563843207013015
31	23.241659545525888	27.5364340814891	27.105096411526365	22.116809961458642
32	22.073211140370763	27.699784331165016	27.4434232631683	22.78358126529592
33	22.391773193119523	27.163809490591373	27.713735953913137	22.73068136237596
34	22.343523831115604	27.57770763211896	27.26728402597327	22.811484510792162
35	22.654528754875802	27.770705080134633	27.122535939961516	22.45223022502805
36	22.792301029513496	27.99509367933358	26.64178627276585	22.570819018387077
37	23.363154926957442	26.96034832551461	26.948140655610004	22.72835609191794
38	23.39163949006819	27.565499962214357	26.83536503839603	22.20749550932143
39	22.666155107165903	27.316114705591694	27.4783023200386	22.539427867203806
40	22.837062485830383	27.10335245868285	27.49516053085925	22.564424524627523
41	22.341198560657585	27.345180586316946	27.66432395668021	22.649296896345255
42	23.06377635548735	26.884195718014453	27.16439080820588	22.88763711829232
43	22.701034164036205	26.964417548816144	27.312626799904667	23.021921487242984
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	3.5
2	5.0
3	19.5
4	34.0
5	34.0
6	46.5
7	59.0
8	61.0
9	63.0
10	102.0
11	141.0
12	141.0
13	176.5
14	212.0
15	417.5
16	623.0
17	715.0
18	807.0
19	807.0
20	870.0
21	933.0
22	1229.5
23	1526.0
24	1930.5
25	2335.0
26	2335.0
27	2977.0
28	3619.0
29	4978.5
30	6338.0
31	6862.0
32	7386.0
33	7386.0
34	8214.0
35	9042.0
36	9858.5
37	10675.0
38	11900.5
39	13126.0
40	13126.0
41	13928.5
42	14731.0
43	13982.5
44	13234.0
45	13434.5
46	13635.0
47	13635.0
48	13446.0
49	13257.0
50	13473.0
51	13689.0
52	13617.5
53	13546.0
54	13546.0
55	11427.5
56	9309.0
57	8133.5
58	6958.0
59	6062.5
60	5167.0
61	5167.0
62	4609.5
63	4052.0
64	3325.0
65	2598.0
66	2227.5
67	1857.0
68	1857.0
69	1498.0
70	1139.0
71	954.0
72	769.0
73	638.0
74	507.0
75	507.0
76	389.5
77	272.0
78	212.5
79	153.0
80	122.5
81	92.0
82	92.0
83	70.0
84	48.0
85	45.5
86	43.0
87	32.5
88	22.0
89	22.0
90	18.5
91	15.0
92	9.5
93	4.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	172023.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.187464779884785
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.94749023060548	56.83553870985414
2	5.078432561571346	6.41786557242619
3	1.4811127455389606	2.807632783313446
4	0.7145538363214209	1.8060338146356536
5	0.452593765765914	1.4299126316964557
6	0.33268928324966873	1.2613075420790134
7	0.25276270611926804	1.1180004213405732
8	0.2098029014109169	1.0605530754895962
9	0.14886014107416962	0.8465485429127496
>10	1.194877358452981	15.11477367448438
>50	0.13487328040176536	5.761706524400643
>100	0.0519511894880874	5.540126707367158
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	341	0.19822930654621765	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	336	0.19532271847369245	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	333	0.19357876563017737	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	306	0.17788319003854136	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	293	0.17032606104997586	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	288	0.1674194729774507	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	257	0.14939862692779454	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	252	0.14649203885526935	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	244	0.14184149793922907	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	226	0.13137778087813837	No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT	198	0.11510088767199736	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	191	0.1110316643704621	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	189	0.10986902914145201	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	189	0.10986902914145201	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	185	0.10754375868343186	No Hit
CATCAAAAGTTCCCAAAGAGGGCTTGCTCTCTCTTCACCTGCT	177	0.10289321776739158	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	175	0.10173058253838149	No Hit
CTGTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGC	175	0.10173058253838149	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	5.813176145050372E-4	0.0	0.0
6	0.0	0.0	5.813176145050372E-4	0.0	0.0
7	0.0	0.0	0.0011626352290100743	0.0	0.0
8	0.0	0.0	0.0011626352290100743	5.813176145050372E-4	0.0
9	0.0	0.0	0.0023252704580201487	5.813176145050372E-4	0.0
10	0.0011626352290100743	0.0	0.0023252704580201487	5.813176145050372E-4	0.0
11	0.0011626352290100743	0.0	0.0023252704580201487	5.813176145050372E-4	0.0
12	0.0011626352290100743	0.0	0.0023252704580201487	5.813176145050372E-4	0.0
13	0.0011626352290100743	0.0	0.0029065880725251854	5.813176145050372E-4	0.0
14	0.0011626352290100743	0.0	0.0029065880725251854	5.813176145050372E-4	0.0
15	0.0011626352290100743	0.0	0.0029065880725251854	5.813176145050372E-4	0.0
16	0.0011626352290100743	0.0	0.0034879056870302226	5.813176145050372E-4	0.0
17	0.0011626352290100743	0.0	0.0034879056870302226	5.813176145050372E-4	0.0
18	0.0011626352290100743	0.0	0.0034879056870302226	5.813176145050372E-4	0.0
19	0.0011626352290100743	0.0	0.0034879056870302226	5.813176145050372E-4	0.0
20	0.0011626352290100743	0.0	0.0034879056870302226	5.813176145050372E-4	0.0
21	0.0011626352290100743	0.0	0.0034879056870302226	5.813176145050372E-4	0.0
22	0.0011626352290100743	0.0	0.0034879056870302226	5.813176145050372E-4	0.0
23	0.0011626352290100743	0.0	0.00406922330153526	5.813176145050372E-4	0.0
24	0.0011626352290100743	0.0	0.00406922330153526	5.813176145050372E-4	0.0
25	0.0011626352290100743	0.0	0.00406922330153526	5.813176145050372E-4	0.0
26	0.0011626352290100743	0.0	0.00406922330153526	5.813176145050372E-4	0.0
27	0.0011626352290100743	0.0	0.00406922330153526	5.813176145050372E-4	0.0
28	0.0011626352290100743	0.0	0.00406922330153526	0.004650540916040297	0.0
29	0.0011626352290100743	0.0	0.00406922330153526	0.014532940362625929	0.0
30	0.0011626352290100743	0.0	0.00406922330153526	0.027321927881736745	0.0
31	0.0017439528435151113	0.0	0.00406922330153526	0.07731524272916994	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGAGA	20	0.001837588	37.0	2
GGACCGT	20	0.001837588	37.0	6
GGTATCA	100	0.0	33.3	1
CTATAGT	25	0.0054835193	29.599998	4
CTCTCCT	25	0.0054835193	29.599998	15
AGTACTG	25	0.0054835193	29.599998	5
TAGGTAC	35	8.8386383E-4	26.42857	3
ACCATGC	40	0.0019245535	23.125002	8
GGGGCTT	45	0.0038127925	20.555555	36
AAACGTC	55	5.1186973E-4	20.181818	15
GTCACGA	55	5.1186973E-4	20.181818	19
CGTCACG	55	5.1186973E-4	20.181818	18
CAAACGT	70	1.2115659E-4	18.5	14
GGTATTC	80	1.6036858E-5	18.5	21
ACGTCAC	60	9.193354E-4	18.5	17
CCTCTTG	70	1.2115659E-4	18.5	15
TTGGCTA	50	0.00701118	18.499998	22
GTACCTA	50	0.00701118	18.499998	1
TAAAGCT	50	0.00701118	18.499998	27
TGGACAG	50	0.00701118	18.499998	5
>>END_MODULE