FastQCFastQC Report
Fri 10 Feb 2017
ERR1632788.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632788.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200844
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6430.3201489713409412No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5310.2643842982613372No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2970.14787596343430723No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC2160.10754615522495072No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA900.030.8333321
TGTATAC453.9866372E-628.7777773
TATACTG405.9161175E-527.755
GTATCAA3550.026.5774651
ATCTAAT509.042395E-625.9000021
ATTACCC603.70661E-521.5833323
GCTCGGA705.0674043E-621.14285911
GTCAGTG450.003814986420.55555512
AATAGCG450.003814986420.5555556
TTACACT450.003814986420.5555554
ATACTCT555.1228056E-420.1818186
GCATAAG500.00701517318.51
CTGCTCG1002.8456816E-718.59
ATAGCGG500.00701517318.57
ATTCGGA609.200677E-418.536
ATGTATA500.00701517318.52
TAACCCA500.00701517318.55
AACATAT801.6058704E-518.55
CATACAC500.00701517318.53
TGCTCGG801.6058704E-518.510
Produced by FastQC (version 0.11.5)