##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632788.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	200844
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.47563282945968	33.0	33.0	33.0	14.0	33.0
2	30.360598275278324	33.0	33.0	33.0	27.0	33.0
3	30.82229989444544	33.0	33.0	33.0	27.0	33.0
4	31.01915914839378	33.0	33.0	33.0	27.0	33.0
5	31.121965306406963	33.0	33.0	33.0	27.0	33.0
6	33.36985421521181	37.0	33.0	37.0	27.0	37.0
7	33.634736412339926	37.0	33.0	37.0	27.0	37.0
8	33.749945231124656	37.0	33.0	37.0	27.0	37.0
9	33.85726733185955	37.0	33.0	37.0	27.0	37.0
10	33.94757124932784	37.0	33.0	37.0	27.0	37.0
11	33.88842086395412	37.0	33.0	37.0	27.0	37.0
12	33.956080340961144	37.0	33.0	37.0	27.0	37.0
13	33.93709545717074	37.0	33.0	37.0	27.0	37.0
14	33.93966461532334	37.0	33.0	37.0	27.0	37.0
15	33.96587401167075	37.0	33.0	37.0	27.0	37.0
16	33.969772559797654	37.0	33.0	37.0	27.0	37.0
17	33.98116448586963	37.0	33.0	37.0	27.0	37.0
18	33.961298321085025	37.0	33.0	37.0	27.0	37.0
19	33.964923024835194	37.0	33.0	37.0	27.0	37.0
20	33.950712991177234	37.0	33.0	37.0	27.0	37.0
21	33.96692955726833	37.0	33.0	37.0	27.0	37.0
22	33.97319810400111	37.0	33.0	37.0	27.0	37.0
23	33.93961980442533	37.0	33.0	37.0	27.0	37.0
24	33.91766246440023	37.0	33.0	37.0	27.0	37.0
25	33.915461751409055	37.0	33.0	37.0	27.0	37.0
26	33.829643902730474	37.0	33.0	37.0	27.0	37.0
27	33.761322220230625	37.0	33.0	37.0	27.0	37.0
28	33.71655613311824	37.0	33.0	37.0	27.0	37.0
29	33.75438648901635	37.0	33.0	37.0	27.0	37.0
30	33.71281193364004	37.0	33.0	37.0	27.0	37.0
31	33.693169823345485	37.0	33.0	37.0	27.0	37.0
32	33.746350401306486	37.0	33.0	37.0	27.0	37.0
33	33.7082063691223	37.0	33.0	37.0	27.0	37.0
34	33.60725239489355	37.0	33.0	37.0	27.0	37.0
35	33.59981876481249	37.0	33.0	37.0	27.0	37.0
36	33.54901316444604	37.0	33.0	37.0	27.0	37.0
37	33.610787476847705	37.0	33.0	37.0	27.0	37.0
38	33.50331600645277	37.0	33.0	37.0	27.0	37.0
39	33.54679253550019	37.0	33.0	37.0	27.0	37.0
40	33.54288900838462	37.0	33.0	37.0	27.0	37.0
41	33.584856903865685	37.0	33.0	37.0	27.0	37.0
42	33.46084025412758	37.0	33.0	37.0	22.0	37.0
43	33.18392882037801	37.0	33.0	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	7.0
15	11.0
16	21.0
17	13.0
18	20.0
19	36.0
20	80.0
21	227.0
22	539.0
23	1029.0
24	1879.0
25	2920.0
26	3992.0
27	5365.0
28	6890.0
29	8688.0
30	10445.0
31	12531.0
32	15041.0
33	18672.0
34	24693.0
35	37577.0
36	50168.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.70970504471132	21.45894325944514	12.689450518810618	25.14190117703292
2	16.885742168050825	24.14062655593396	35.403098922546846	23.570532353468362
3	18.546234888769394	27.347593156877974	30.09350540718169	24.012666547170937
4	13.775865846129335	19.343370974487662	37.73575511342136	29.14500806596164
5	15.241182211069287	36.93961482543666	34.69160144191512	13.127601521578939
6	29.90529963553803	40.008663440282014	17.0903786022983	12.99565832188166
7	26.63559777737946	32.7139471430563	23.14682041783673	17.50363466172751
8	24.70026488219713	36.13003126804883	20.249546912031228	18.92015693772281
9	26.240266077154406	15.164505785584831	20.03345880384776	38.561769333413
10	14.783115253629683	28.36878373264822	34.181255103463386	22.666845910258708
11	34.67317918384418	23.470952580111927	23.82346497779371	18.032403258250184
12	22.78186054848539	25.88874947720619	30.317559897233675	21.011830077074745
13	28.191531736073767	22.247615064428114	25.90269064547609	23.658162554022027
14	22.042978630180638	20.921212483320385	27.493975423711937	29.541833462787036
15	24.073410208918364	28.5007269323455	24.291987811435742	23.133875047300393
16	23.043257453546033	28.409113540857582	25.231522973053718	23.31610603254267
17	22.428352353070043	27.565174961661786	26.470295353607774	23.536177331660394
18	22.979526398597915	27.299794865666886	27.634382904144513	22.08629583159069
19	24.67985102865906	26.4528688932704	27.139969329429807	21.727310748640736
20	24.095815657923563	26.28009798649698	27.37796498775169	22.24612136782777
21	23.54314791579534	27.083208858616636	27.29083268606481	22.082810539523212
22	23.047240644480294	26.829280436557728	27.1429567226305	22.98052219633148
23	22.77289836888331	26.97665850112525	27.64732827468085	22.603114855310587
24	23.141343530302123	27.530819939853817	26.13321782079624	23.194618709047816
25	23.277767819800445	26.915914839377823	27.661269442950747	22.145047897870985
26	23.488379040449303	27.176315946704904	27.133496644161635	22.201808368684155
27	23.1672342713748	26.916412738244606	26.89599888470654	23.020354105674055
28	22.874469737706875	26.758080898607872	27.52932624325347	22.838123120431778
29	23.899643504411383	26.944295074784407	26.65700742865109	22.499053992153115
30	23.616339049212325	26.70032463006114	27.205194080978274	22.478142239748262
31	23.28822369600287	26.96819422038995	27.00653243313218	22.737049650474994
32	22.400967915397025	26.847702694628666	27.22361633904921	23.527713050925094
33	22.453247296409153	26.794427515882973	27.87885124773456	22.873473939973312
34	23.1363645416343	26.361255501782477	27.591563601601244	22.910816354981975
35	22.85505168190237	27.073748780147778	27.192746609308717	22.878452928641135
36	23.81848598912589	26.917408535978172	26.58082890203342	22.68327657286252
37	23.290215291469995	26.466312162673518	27.05233912887614	23.191133416980342
38	23.4689609846448	26.83674891955946	26.731194359801634	22.963095735994106
39	22.796797514488855	26.822807751289556	27.2714146302603	23.108980103961283
40	22.88641931050965	27.2798789109956	27.368504909282827	22.465196869211926
41	23.0442532512796	26.368226085917428	27.222620541315646	23.364900121487324
42	22.672322797793313	27.13399454302842	27.217641552647827	22.97604110653044
43	23.35095895321742	26.05255820437753	27.526836748919557	23.069646093485492
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	6.5
2	12.0
3	32.5
4	53.0
5	53.0
6	68.0
7	83.0
8	107.5
9	132.0
10	157.0
11	182.0
12	182.0
13	294.0
14	406.0
15	700.5
16	995.0
17	1096.5
18	1198.0
19	1198.0
20	1147.5
21	1097.0
22	1168.5
23	1240.0
24	1759.0
25	2278.0
26	2278.0
27	2909.5
28	3541.0
29	4493.5
30	5446.0
31	6458.0
32	7470.0
33	7470.0
34	8822.0
35	10174.0
36	11287.5
37	12401.0
38	13483.0
39	14565.0
40	14565.0
41	15334.0
42	16103.0
43	16573.0
44	17043.0
45	16881.5
46	16720.0
47	16720.0
48	16288.0
49	15856.0
50	15578.0
51	15300.0
52	14586.0
53	13872.0
54	13872.0
55	12934.5
56	11997.0
57	10689.5
58	9382.0
59	8464.0
60	7546.0
61	7546.0
62	6523.5
63	5501.0
64	4577.0
65	3653.0
66	3110.5
67	2568.0
68	2568.0
69	2099.0
70	1630.0
71	1331.0
72	1032.0
73	825.0
74	618.0
75	618.0
76	461.0
77	304.0
78	248.0
79	192.0
80	150.5
81	109.0
82	109.0
83	84.5
84	60.0
85	54.0
86	48.0
87	37.0
88	26.0
89	26.0
90	17.5
91	9.0
92	5.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	200844.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.595349585776454
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.41933244636319	54.95867657895901
2	7.288806076573725	9.270683410052754
3	2.1325804561272004	4.068665988816118
4	1.1020542827709978	2.8034210950129492
5	0.6308453205091898	2.005941434616656
6	0.4257882566704563	1.6246891819485583
7	0.28461643359770555	1.267019711475211
8	0.22604545679186863	1.150035187756433
9	0.19529397834014778	1.1177809942084878
>10	1.1687425112248817	14.176252627069408
>50	0.09516455888929393	4.221286315041748
>100	0.028747627164474206	2.595435447883345
>500	0.00198259497686029	0.7401120271593506
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	643	0.3201489713409412	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	531	0.2643842982613372	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	297	0.14787596343430723	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	216	0.10754615522495072	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	4.978988667821792E-4	0.0	0.0
2	0.0	0.0	4.978988667821792E-4	0.0	0.0
3	0.0	0.0	4.978988667821792E-4	0.0	0.0
4	0.0	0.0	4.978988667821792E-4	0.0	0.0
5	0.0	0.0	4.978988667821792E-4	0.0	0.0
6	0.0	0.0	0.0014936966003465375	0.0	0.0
7	0.0	0.0	0.002987393200693075	0.0	0.0
8	0.0	0.0	0.0034852920674752546	0.0	0.0
9	0.0	0.0	0.0034852920674752546	0.0	0.0
10	9.957977335643584E-4	0.0	0.004481089801039613	4.978988667821792E-4	0.0
11	9.957977335643584E-4	0.0	0.00597478640138615	4.978988667821792E-4	0.0
12	9.957977335643584E-4	0.0	0.006472685268168329	4.978988667821792E-4	0.0
13	9.957977335643584E-4	0.0	0.006970584134950509	4.978988667821792E-4	0.0
14	9.957977335643584E-4	0.0	0.006970584134950509	4.978988667821792E-4	0.0
15	9.957977335643584E-4	0.0	0.009957977335643584	4.978988667821792E-4	0.0
16	9.957977335643584E-4	0.0	0.011451673935990121	4.978988667821792E-4	0.0
17	9.957977335643584E-4	0.0	0.0119495728027723	4.978988667821792E-4	0.0
18	9.957977335643584E-4	0.0	0.012945370536336659	4.978988667821792E-4	0.0
19	9.957977335643584E-4	0.0	0.013941168269901019	4.978988667821792E-4	0.0
20	9.957977335643584E-4	0.0	0.014936966003465377	9.957977335643584E-4	0.0
21	9.957977335643584E-4	0.0	0.014936966003465377	9.957977335643584E-4	0.0
22	9.957977335643584E-4	0.0	0.015434864870247556	9.957977335643584E-4	0.0
23	9.957977335643584E-4	0.0	0.015932763737029735	9.957977335643584E-4	0.0
24	9.957977335643584E-4	0.0	0.015932763737029735	0.001991595467128717	0.0
25	9.957977335643584E-4	0.0	0.015932763737029735	0.001991595467128717	0.0
26	9.957977335643584E-4	0.0	0.015932763737029735	0.002489494333910896	0.0
27	9.957977335643584E-4	0.0	0.015932763737029735	0.002987393200693075	0.0
28	9.957977335643584E-4	0.0	0.015932763737029735	0.009460078468861405	0.0
29	9.957977335643584E-4	0.0	0.015932763737029735	0.01941805580450499	0.0
30	9.957977335643584E-4	0.0	0.015932763737029735	0.03734241500866344	0.0
31	9.957977335643584E-4	0.0	0.015932763737029735	0.10505666089103981	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	90	0.0	30.833332	1
TGTATAC	45	3.9866372E-6	28.777777	3
TATACTG	40	5.9161175E-5	27.75	5
GTATCAA	355	0.0	26.577465	1
ATCTAAT	50	9.042395E-6	25.900002	1
ATTACCC	60	3.70661E-5	21.583332	3
GCTCGGA	70	5.0674043E-6	21.142859	11
GTCAGTG	45	0.0038149864	20.555555	12
AATAGCG	45	0.0038149864	20.555555	6
TTACACT	45	0.0038149864	20.555555	4
ATACTCT	55	5.1228056E-4	20.181818	6
GCATAAG	50	0.007015173	18.5	1
CTGCTCG	100	2.8456816E-7	18.5	9
ATAGCGG	50	0.007015173	18.5	7
ATTCGGA	60	9.200677E-4	18.5	36
ATGTATA	50	0.007015173	18.5	2
TAACCCA	50	0.007015173	18.5	5
AACATAT	80	1.6058704E-5	18.5	5
CATACAC	50	0.007015173	18.5	3
TGCTCGG	80	1.6058704E-5	18.5	10
>>END_MODULE