FastQCFastQC Report
Fri 10 Feb 2017
ERR1632787.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632787.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366836
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14190.3868213588633613No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9280.2529740810607465No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5410.14747734682528432No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAACC250.005491526329.5999983
CGAATTA250.005491526329.59999815
GGTATCA2600.029.1730771
ATACGCC358.857838E-426.428576
AATACGC358.857838E-426.428575
GTATCAA6900.022.2536221
TTAACGG603.7173882E-521.58333435
AGCTTCG450.003820918720.55555521
CGAGACA450.003820918720.55555523
TTTGACC450.003820918720.55555510
GTACATG1001.2825694E-820.3499981
ATTAGAG759.237594E-619.7333343
ATAATAC759.237594E-619.7333343
TCTAATA1052.2468157E-819.380952
AACGGCC701.21632766E-418.537
GTATAAT801.6117858E-518.51
GCTTCGC500.007025976718.49999822
TACTGAG500.007025976718.4999987
TACATGC1258.521965E-917.762
TAGAGGT953.5928606E-617.52631619
Produced by FastQC (version 0.11.5)