##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632787.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	366836
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.658329607781134	33.0	33.0	33.0	27.0	33.0
2	31.185963209717695	33.0	33.0	33.0	27.0	33.0
3	31.626994079098015	33.0	33.0	33.0	27.0	33.0
4	31.771273811730584	33.0	33.0	33.0	27.0	33.0
5	31.881598316413875	33.0	33.0	33.0	33.0	33.0
6	34.747538409534506	37.0	37.0	37.0	27.0	37.0
7	34.90604793422674	37.0	37.0	37.0	27.0	37.0
8	35.083704979882015	37.0	37.0	37.0	33.0	37.0
9	35.16188432978225	37.0	37.0	37.0	33.0	37.0
10	35.17419773413732	37.0	37.0	37.0	33.0	37.0
11	35.2242064573815	37.0	37.0	37.0	33.0	37.0
12	35.154742173614366	37.0	37.0	37.0	33.0	37.0
13	35.19507082183864	37.0	37.0	37.0	33.0	37.0
14	35.16559716058402	37.0	37.0	37.0	33.0	37.0
15	35.18635575570555	37.0	37.0	37.0	33.0	37.0
16	35.19872368033672	37.0	37.0	37.0	33.0	37.0
17	35.16987700225714	37.0	37.0	37.0	33.0	37.0
18	35.17092924358569	37.0	37.0	37.0	33.0	37.0
19	35.17758616929636	37.0	37.0	37.0	33.0	37.0
20	35.17136540579442	37.0	37.0	37.0	33.0	37.0
21	35.169511716407335	37.0	37.0	37.0	33.0	37.0
22	35.18585689517932	37.0	37.0	37.0	33.0	37.0
23	35.17222137412904	37.0	37.0	37.0	33.0	37.0
24	35.15694751878223	37.0	37.0	37.0	33.0	37.0
25	35.13621073177114	37.0	37.0	37.0	33.0	37.0
26	35.1176656598589	37.0	37.0	37.0	33.0	37.0
27	35.050886499689234	37.0	37.0	37.0	27.0	37.0
28	35.02117022320601	37.0	37.0	37.0	27.0	37.0
29	35.047470804392155	37.0	37.0	37.0	27.0	37.0
30	35.025638159831644	37.0	37.0	37.0	27.0	37.0
31	35.01827247053179	37.0	37.0	37.0	27.0	37.0
32	35.037785277344646	37.0	37.0	37.0	27.0	37.0
33	35.01968454568254	37.0	37.0	37.0	27.0	37.0
34	34.94844835294246	37.0	37.0	37.0	27.0	37.0
35	34.94557786040629	37.0	37.0	37.0	27.0	37.0
36	34.9193154434134	37.0	37.0	37.0	27.0	37.0
37	34.9561084517332	37.0	37.0	37.0	27.0	37.0
38	34.80465930279471	37.0	37.0	37.0	27.0	37.0
39	34.87493048664798	37.0	37.0	37.0	27.0	37.0
40	34.92130815950452	37.0	37.0	37.0	27.0	37.0
41	34.920283178314016	37.0	37.0	37.0	27.0	37.0
42	34.82589495033203	37.0	37.0	37.0	27.0	37.0
43	34.60709417832492	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	14.0
16	11.0
17	18.0
18	18.0
19	19.0
20	24.0
21	93.0
22	240.0
23	571.0
24	1180.0
25	2011.0
26	3240.0
27	4947.0
28	6803.0
29	9231.0
30	11645.0
31	15064.0
32	18865.0
33	24545.0
34	34331.0
35	61653.0
36	172312.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.10610736132767	20.744147248361667	13.481773871702888	24.66797151860777
2	15.832415575352474	23.492241764712297	38.23397921687076	22.44136344306448
3	17.657754418868375	27.37817444307538	30.93044303176351	24.03362810629273
4	12.42162710311965	18.148982106445388	40.00261697325235	29.42677381718261
5	13.486408095170594	37.43498457076186	35.792288652149736	13.286318681917805
6	28.7103773893511	40.641867210415555	17.496919604400876	13.150835795832469
7	25.830071203480575	32.71461906683095	24.084877165817968	17.3704325638705
8	23.820999029539085	36.41082118439848	21.205388784088804	18.562791001973633
9	25.94292817498828	15.455135264804982	20.473181476190998	38.12875508401575
10	14.525019354698014	28.469942971791205	34.71169678003249	22.293340893478284
11	33.428289480857934	23.956754517004875	24.42290287758017	18.192053124557024
12	22.197657808939145	26.155829853122377	30.695733243193146	20.950779094745336
13	27.732556237664785	22.374030902092485	26.73183657002039	23.16157629022233
14	21.777306480280014	21.450184823735945	28.17798689332563	28.594521802658406
15	23.743035034729417	28.91292021502797	25.00954104831587	22.334503701926746
16	22.565397071170768	28.90801339017981	25.563194452016706	22.96339508663272
17	21.947409741682932	28.032962958924422	26.680042307734247	23.339584991658395
18	22.53895473726679	27.55999956383779	28.082031207406033	21.819014491489387
19	24.407091997513874	26.67377247598382	27.91519916256856	21.003936363933747
20	24.07288270507802	26.499852795254554	28.036234175489867	21.391030324177564
21	22.469441385251173	27.770993032308716	27.477673946940868	22.281891635499242
22	22.336684512970372	27.45940965445049	27.601434973666706	22.60247085891243
23	22.31378599701229	27.358001940921827	28.239322203927642	22.08888985813824
24	22.8734366310831	27.599526764003535	26.93901361916497	22.5880229857484
25	22.94731160518597	27.063592450032164	28.136006280735803	21.853089664046056
26	22.697881342071117	27.340010249811904	27.713746742413502	22.248361665703477
27	22.676345833015297	27.359637549204553	27.296666630319816	22.667349987460337
28	22.135777295576226	27.414703028056138	28.117469386864975	22.332050289502668
29	23.20246649729034	27.408160594925253	27.232332704532812	22.15704020325159
30	22.687522489613887	27.369723800281324	27.70311528857582	22.23963842152897
31	22.805558887350205	27.316021328332006	27.668222311877788	22.21019747244
32	21.886619633841825	27.170179589789445	27.841324188465688	23.101876587903043
33	22.02510113511215	26.91666030596779	28.488752467042495	22.569486091877568
34	22.37893772694065	26.75337207907621	28.24095781221036	22.626732381772783
35	22.37239529380977	27.684305793324537	27.630058118614315	22.31324079425138
36	22.807739698393835	27.513112126399808	27.46240826963548	22.216739905570883
37	22.77393712721761	26.648965750362557	27.62842251033159	22.948674612088237
38	22.83799845162416	27.15654952076677	27.475220534516787	22.53023149309228
39	22.302064137652795	26.924838347381392	28.019605491282206	22.75349202368361
40	22.256267105736622	27.38417167344536	28.24368382601489	22.115877394803128
41	22.407560871888254	26.66096021110251	28.02069589680402	22.910783020205212
42	22.25572190297572	27.152733101440425	27.919015581894907	22.672529413688952
43	22.635728227327743	26.343924805635215	28.110381750973186	22.909965216063853
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	9.0
2	16.0
3	85.0
4	154.0
5	154.0
6	183.0
7	212.0
8	223.5
9	235.0
10	320.5
11	406.0
12	406.0
13	575.5
14	745.0
15	1312.0
16	1879.0
17	2222.5
18	2566.0
19	2566.0
20	2506.0
21	2446.0
22	2922.0
23	3398.0
24	4428.0
25	5458.0
26	5458.0
27	6891.5
28	8325.0
29	10272.0
30	12219.0
31	14274.5
32	16330.0
33	16330.0
34	18321.0
35	20312.0
36	22018.5
37	23725.0
38	25604.0
39	27483.0
40	27483.0
41	28665.5
42	29848.0
43	29757.0
44	29666.0
45	29063.5
46	28461.0
47	28461.0
48	27592.5
49	26724.0
50	26939.0
51	27154.0
52	26150.5
53	25147.0
54	25147.0
55	22846.5
56	20546.0
57	18089.5
58	15633.0
59	13858.0
60	12083.0
61	12083.0
62	10695.5
63	9308.0
64	7518.0
65	5728.0
66	4885.0
67	4042.0
68	4042.0
69	3272.0
70	2502.0
71	2090.5
72	1679.0
73	1388.5
74	1098.0
75	1098.0
76	810.5
77	523.0
78	427.5
79	332.0
80	254.5
81	177.0
82	177.0
83	154.0
84	131.0
85	100.5
86	70.0
87	56.0
88	42.0
89	42.0
90	32.0
91	22.0
92	15.0
93	8.0
94	4.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	366836.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.74733052244327
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.69809852422924	56.28316235334493
2	5.3310053913015665	6.690127146098527
3	1.4634556758391992	2.7548381099048345
4	0.7088069177817103	1.77902967786573
5	0.463781495086737	1.4550525381200194
6	0.33146663791453784	1.2479188011831919
7	0.24260565902680997	1.0656000231499292
8	0.18546469512967478	0.9309931620436701
9	0.15321558136785396	0.865248185274933
>10	1.244551123696434	15.652009572709225
>50	0.12669495877284823	5.3920899803590725
>100	0.0495381672710221	5.0895035617063895
>500	8.767817216110106E-4	0.4040904081800177
>1k	4.383908608055053E-4	0.3903364800595269
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1419	0.3868213588633613	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	928	0.2529740810607465	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	541	0.14747734682528432	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0010904055218135624	0.0	0.0
2	0.0	0.0	0.0013630069022669531	0.0	0.0
3	0.0	0.0	0.0013630069022669531	0.0	0.0
4	0.0	0.0	0.0019082096631737344	0.0	0.0
5	0.0	0.0	0.002180811043627125	0.0	0.0
6	0.0	0.0	0.0038164193263474687	0.0	0.0
7	0.0	0.0	0.007087635891788156	0.0	0.0
8	0.0	0.0	0.00817804141360172	0.0	0.0
9	0.0	0.0	0.010631453837682233	8.178041413601718E-4	0.0
10	8.178041413601718E-4	0.0	0.011449257979042405	0.0010904055218135624	0.0
11	8.178041413601718E-4	0.0	0.01690128558811022	0.0010904055218135624	0.0
12	8.178041413601718E-4	0.0	0.01799169110992378	0.0010904055218135624	0.0
13	8.178041413601718E-4	0.0	0.019354698012190735	0.0010904055218135624	0.0
14	8.178041413601718E-4	0.0	0.020717704914457687	0.0010904055218135624	0.0
15	8.178041413601718E-4	0.0	0.02180811043627125	0.0010904055218135624	0.0
16	8.178041413601718E-4	0.0	0.023443718718991594	0.0010904055218135624	0.0
17	8.178041413601718E-4	0.0	0.023716320099444983	0.0010904055218135624	0.0
18	8.178041413601718E-4	0.0	0.025079327001711938	0.0010904055218135624	0.0
19	8.178041413601718E-4	0.0	0.02589713114307211	0.0010904055218135624	0.0
20	8.178041413601718E-4	0.0	0.02698753666488567	0.0013630069022669531	0.0
21	8.178041413601718E-4	0.0	0.027532739425792454	0.0013630069022669531	0.0
22	8.178041413601718E-4	0.0	0.028077942186699234	0.0016356082827203436	0.0
23	8.178041413601718E-4	0.0	0.028077942186699234	0.0016356082827203436	0.0
24	8.178041413601718E-4	0.0	0.028077942186699234	0.002998615184987297	0.0
25	8.178041413601718E-4	0.0	0.028350543567152622	0.002998615184987297	0.0
26	8.178041413601718E-4	0.0	0.028350543567152622	0.003543817945894078	0.0
27	8.178041413601718E-4	0.0	0.028350543567152622	0.005179426228614422	0.0
28	8.178041413601718E-4	0.0	0.028623144947606014	0.010631453837682233	0.0
29	8.178041413601718E-4	0.0	0.028623144947606014	0.029986151849872966	0.0
30	8.178041413601718E-4	0.0	0.028623144947606014	0.056701087134305245	0.0
31	8.178041413601718E-4	0.0	0.028623144947606014	0.12703224329128002	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTAACC	25	0.0054915263	29.599998	3
CGAATTA	25	0.0054915263	29.599998	15
GGTATCA	260	0.0	29.173077	1
ATACGCC	35	8.857838E-4	26.42857	6
AATACGC	35	8.857838E-4	26.42857	5
GTATCAA	690	0.0	22.253622	1
TTAACGG	60	3.7173882E-5	21.583334	35
AGCTTCG	45	0.0038209187	20.555555	21
CGAGACA	45	0.0038209187	20.555555	23
TTTGACC	45	0.0038209187	20.555555	10
GTACATG	100	1.2825694E-8	20.349998	1
ATTAGAG	75	9.237594E-6	19.733334	3
ATAATAC	75	9.237594E-6	19.733334	3
TCTAATA	105	2.2468157E-8	19.38095	2
AACGGCC	70	1.21632766E-4	18.5	37
GTATAAT	80	1.6117858E-5	18.5	1
GCTTCGC	50	0.0070259767	18.499998	22
TACTGAG	50	0.0070259767	18.499998	7
TACATGC	125	8.521965E-9	17.76	2
TAGAGGT	95	3.5928606E-6	17.526316	19
>>END_MODULE