FastQCFastQC Report
Fri 10 Feb 2017
ERR1632786.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632786.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383113
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9060.23648375283532533No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6400.16705254063422542No Hit
GCCTTAGGCCGTATGACAAAATGAAGAGACTGAAATGACAGCG4540.11850289601240366No Hit
CTTTAAAGCCTTAGGCCGTATGACAAAATGAAGAGACTGAAAT3900.1017976419489811No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGTG250.00549182729.610
ATTAGAG556.2434447E-726.909093
ATGCCCG358.8585605E-426.4285729
GGTATCA2450.024.1632651
TTAGCCG400.001928850523.12529
TATTAGA802.7137503E-823.1252
TTAACGG400.001928850523.12535
TTTAACG502.6975482E-422.234
GGAGTTA502.6975482E-422.225
GTATCAA5950.021.1428591
GGGTGTC705.087286E-621.1428577
ACTATCG450.003821221920.55555711
CGCGGCT450.003821221920.55555710
AGCTTCG450.003821221920.55555721
CCTACCG450.003821221920.55555724
CCGCTGA450.003821221920.55555726
CCCGTTC450.003821221920.55555732
TTCGCCG450.003821221920.55555724
CCGTATG1351.8189894E-1220.5555559
GGCCGTA1450.020.4137947
Produced by FastQC (version 0.11.5)