FastQCFastQC Report
Fri 10 Feb 2017
ERR1632785.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632785.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379179
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7900.208344871419567No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT7170.18909275038965764No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC6120.16140134342882914No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC6090.16061016037280545No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG5880.15507187898063976No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5860.1545444236099573No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC4890.12896283813185858No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT4580.12078727988628063No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA4430.11683136460616227No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT4320.11393036006740878No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC4250.11208426627002024No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC4220.11129308321399654No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA4030.1062822571925133No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA3950.1041724357097835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTCGC556.2431536E-726.9090922
TTTCCGA358.858392E-426.4285717
ATAACCG358.858392E-426.428576
ATTTCCG358.858392E-426.4285716
TAACGGC509.070123E-625.90000236
CGTTATA509.070123E-625.9000022
GTATCAA5250.025.0190491
GCCGCTC601.3334611E-624.66666627
TTAACGG551.8983023E-523.54545435
CGGCGAT400.001928813623.12500211
ACCAAGC400.001928813623.12500235
ATAGTAC400.001928813623.1250023
GGTATCA2050.021.6585371
CGGCAAG1400.021.14285718
CCGCTCT705.0870567E-621.14285728
CGCTCTC705.0870567E-621.14285729
CTTATCC705.0870567E-621.1428573
TCTAAGC705.0870567E-621.1428573
TAAGCAG705.0870567E-621.1428575
CGAACTA1151.3460522E-1020.91304429
Produced by FastQC (version 0.11.5)