FastQCFastQC Report
Fri 10 Feb 2017
ERR1632784.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632784.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352073
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12290.34907533380861355No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8770.24909606814495855No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4620.13122278618354716No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTAAA303.594453E-430.83333211
AGGTCGC502.711713E-729.635
GGTATCA2300.027.3478261
GGTCGCC556.2410254E-726.9090936
ATACTTA358.8571274E-426.428576
GGCCTAA358.8571274E-426.4285710
CTTAGGT509.067715E-625.90000230
CGAACCG1200.024.66666631
GCCGGCA451.3206666E-424.66666615
GGCGAAC1250.023.6829
TAGCTCG551.8978022E-523.54545436
CCGGGGG1500.023.43333435
GCGAACC1300.022.7692330
TGCTCGG1155.456968E-1222.5217410
GTATCAA5800.022.3275851
GCTCCGA753.7255268E-722.229
GAATCCA1350.021.92592411
AACCGGG1450.021.68965533
TCCGAGG806.931932E-720.812531
CATAGAC806.931932E-720.81252
Produced by FastQC (version 0.11.5)