##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632784.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	352073
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.44120679518168	33.0	33.0	33.0	27.0	33.0
2	31.027607910859395	33.0	33.0	33.0	27.0	33.0
3	31.4714363214447	33.0	33.0	33.0	27.0	33.0
4	31.633493622061334	33.0	33.0	33.0	27.0	33.0
5	31.748856061100966	33.0	33.0	33.0	27.0	33.0
6	34.56749026480304	37.0	37.0	37.0	27.0	37.0
7	34.756709546031644	37.0	37.0	37.0	27.0	37.0
8	34.927185555268366	37.0	37.0	37.0	27.0	37.0
9	35.01693682844183	37.0	37.0	37.0	27.0	37.0
10	35.07550422781639	37.0	37.0	37.0	33.0	37.0
11	35.083684349552506	37.0	37.0	37.0	33.0	37.0
12	35.02341275814959	37.0	37.0	37.0	27.0	37.0
13	35.041619209652545	37.0	37.0	37.0	27.0	37.0
14	35.02193863204506	37.0	37.0	37.0	27.0	37.0
15	35.03690427837409	37.0	37.0	37.0	27.0	37.0
16	35.047669091353214	37.0	37.0	37.0	27.0	37.0
17	35.00501316488342	37.0	37.0	37.0	27.0	37.0
18	35.0230889616642	37.0	37.0	37.0	27.0	37.0
19	35.027610751179445	37.0	37.0	37.0	27.0	37.0
20	35.03095380787507	37.0	37.0	37.0	27.0	37.0
21	35.034424678972826	37.0	37.0	37.0	27.0	37.0
22	35.03302724150957	37.0	37.0	37.0	27.0	37.0
23	35.011733362115244	37.0	37.0	37.0	27.0	37.0
24	35.00715476619906	37.0	37.0	37.0	27.0	37.0
25	34.989314715982196	37.0	37.0	37.0	27.0	37.0
26	34.950501742536346	37.0	37.0	37.0	27.0	37.0
27	34.895322844978175	37.0	37.0	37.0	27.0	37.0
28	34.86427246622149	37.0	37.0	37.0	27.0	37.0
29	34.909425034012834	37.0	37.0	37.0	27.0	37.0
30	34.86803304996407	37.0	37.0	37.0	27.0	37.0
31	34.84456064509349	37.0	37.0	37.0	27.0	37.0
32	34.87943125431374	37.0	37.0	37.0	27.0	37.0
33	34.87309450028829	37.0	37.0	37.0	27.0	37.0
34	34.79062864803606	37.0	37.0	37.0	27.0	37.0
35	34.795085110190215	37.0	37.0	37.0	27.0	37.0
36	34.74572602840888	37.0	37.0	37.0	27.0	37.0
37	34.796746697417866	37.0	37.0	37.0	27.0	37.0
38	34.65028559418075	37.0	37.0	37.0	27.0	37.0
39	34.72354596915981	37.0	37.0	37.0	27.0	37.0
40	34.75438900455303	37.0	37.0	37.0	27.0	37.0
41	34.76726701564732	37.0	37.0	37.0	27.0	37.0
42	34.67670909158044	37.0	37.0	37.0	27.0	37.0
43	34.436250436699204	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	14.0
16	14.0
17	23.0
18	16.0
19	13.0
20	33.0
21	116.0
22	256.0
23	621.0
24	1268.0
25	2207.0
26	3665.0
27	5213.0
28	7328.0
29	9643.0
30	12218.0
31	15322.0
32	19232.0
33	25072.0
34	34831.0
35	60944.0
36	154024.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.63880786086976	20.01772359709492	13.143581018709188	25.19988752332613
2	16.50822414669685	23.058286207689882	36.716817250967836	23.71667239464543
3	18.448730802986884	26.32664248607533	29.865113200955484	25.359513509982307
4	13.124550874392526	17.835505704782815	38.465886336072344	30.574057084752308
5	14.656903539890875	36.50180502339004	34.67008262491017	14.171208811808913
6	30.31587199245611	39.337864590582065	16.98340969060394	13.362853726357887
7	26.900103103617717	32.0268807889273	22.97108838223891	18.101927725216076
8	24.89483715025009	35.654537553291505	20.442635476165457	19.007989820292952
9	26.14685022708359	14.861974647303258	19.878547914779038	39.11262721083412
10	14.93525490452264	28.0115203381117	33.83730078705269	23.215923970312975
11	35.18758893752148	23.052605567595357	23.52125837539374	18.238547119489425
12	23.041244287406304	25.0882629454687	30.29598975212527	21.57450301499973
13	28.604011099970744	21.9144893246571	25.685014187398636	23.79648538797352
14	22.141146864428684	20.426445651896056	27.53235834613844	29.900049137536815
15	24.145276689777404	28.305209430998683	23.999568271352818	23.549945607871095
16	23.223876866445313	27.9186418725662	24.91784374263292	23.939637518355568
17	22.76857356286906	27.204017348674846	26.020171952975662	24.007237135480423
18	23.572100104239745	26.455877048225794	27.298031942239255	22.67399090529521
19	25.022083488367468	26.010798896819693	26.850113470785885	22.11700414402695
20	24.68720975479517	25.82901841379487	26.79643142189262	22.687340409517347
21	23.55193383190418	26.47717944858027	26.919985343948554	23.050901375566998
22	23.27244633925351	26.24256901267635	27.004342849352263	23.48064179871788
23	23.33805773234528	26.28829816543728	27.281842117969852	23.091801984247585
24	23.536312071644232	26.594768698536953	26.329766838127323	23.539152391691495
25	23.65759373766236	26.29057042147509	27.19720058056142	22.85463526030113
26	23.684008714101907	26.471498808485737	26.715198268540902	23.129294208871457
27	23.530631431549708	26.41952095162083	26.63140882714664	23.41843878968282
28	23.26761779517316	26.31670136590991	27.08983648277488	23.32584435614205
29	24.129370897512732	26.349081014448707	26.38714130308203	23.13440678495653
30	23.99019521519685	26.09856478628012	26.81915398227072	23.09208601625231
31	23.758425099340194	26.197691955929592	26.69588409221951	23.347998852510703
32	22.791580155251896	26.16900472345224	27.10744646706791	23.93196865422796
33	22.870541052565805	26.276652853243505	27.42016570427156	23.432640389919136
34	23.415882501640283	25.957684911935875	27.1386899875878	23.487742598836036
35	23.406509445484318	26.59249644249914	26.881641023310504	23.119353088706035
36	23.8913520775521	26.234616116544014	26.59505273054168	23.278979075362212
37	23.643392137426044	26.083511090029628	26.621183674976496	23.651913097567835
38	23.632030857236995	26.363850678694472	26.51808005726085	23.48603840680768
39	23.213083650265713	26.120151218639315	27.047231682065938	23.61953344902904
40	23.280399235385843	26.53000940145936	27.219071044925343	22.97052031822946
41	23.608172168839985	25.815952941577457	27.01201171347988	23.563863176102686
42	22.88360652478321	26.634249147193906	27.04098297796196	23.441161350060923
43	23.53261965558279	25.779880876977217	27.025929281711463	23.66157018572853
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	6.5
2	8.0
3	61.5
4	115.0
5	115.0
6	156.0
7	197.0
8	192.5
9	188.0
10	259.0
11	330.0
12	330.0
13	480.0
14	630.0
15	1104.0
16	1578.0
17	1806.0
18	2034.0
19	2034.0
20	1879.0
21	1724.0
22	1903.5
23	2083.0
24	2881.5
25	3680.0
26	3680.0
27	4897.5
28	6115.0
29	7501.5
30	8888.0
31	10663.0
32	12438.0
33	12438.0
34	14738.0
35	17038.0
36	18650.5
37	20263.0
38	22138.0
39	24013.0
40	24013.0
41	25235.0
42	26457.0
43	27196.0
44	27935.0
45	27933.0
46	27931.0
47	27931.0
48	27610.5
49	27290.0
50	27134.0
51	26978.0
52	26196.0
53	25414.0
54	25414.0
55	23826.0
56	22238.0
57	19956.0
58	17674.0
59	16375.5
60	15077.0
61	15077.0
62	13259.5
63	11442.0
64	9617.5
65	7793.0
66	6654.5
67	5516.0
68	5516.0
69	4533.5
70	3551.0
71	2898.5
72	2246.0
73	1823.0
74	1400.0
75	1400.0
76	1081.5
77	763.0
78	617.0
79	471.0
80	358.0
81	245.0
82	245.0
83	199.0
84	153.0
85	122.5
86	92.0
87	68.0
88	44.0
89	44.0
90	36.5
91	29.0
92	17.5
93	6.0
94	3.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	352073.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.5245951214948
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.60388493669282	64.08881591409961
2	6.058190583911914	8.666192573663036
3	1.6260325243630482	3.4890395387834747
4	0.7387903926532418	2.1136673485669317
5	0.4240787662287517	1.5166031027067253
6	0.29785666186667414	1.2782446286552305
7	0.20327946907401406	1.0177637205421894
8	0.14184997555897647	0.811660965587978
9	0.11527190309516422	0.7420298577989223
>10	0.7026014924810624	9.579518512528251
>50	0.05704683734247014	2.7674380142368173
>100	0.030318598866909595	3.328115991103786
>500	3.9892893245933674E-4	0.25023643040106486
>1k	3.9892893245933674E-4	0.35067340132600766
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1229	0.34907533380861355	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	877	0.24909606814495855	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	462	0.13122278618354716	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	5.680640094525852E-4	0.0	0.0
2	2.840320047262926E-4	0.0	5.680640094525852E-4	0.0	0.0
3	2.840320047262926E-4	0.0	8.520960141788777E-4	0.0	0.0
4	2.840320047262926E-4	0.0	8.520960141788777E-4	0.0	0.0
5	2.840320047262926E-4	0.0	8.520960141788777E-4	0.0	0.0
6	2.840320047262926E-4	0.0	0.001988224033084048	0.0	0.0
7	2.840320047262926E-4	0.0	0.006532736108704729	2.840320047262926E-4	0.0
8	2.840320047262926E-4	0.0	0.007384832122883606	2.840320047262926E-4	0.0
9	2.840320047262926E-4	0.0	0.008520960141788776	0.0011361280189051703	0.0
10	8.520960141788777E-4	0.0	0.009373056155967655	0.0014201600236314628	0.0
11	8.520960141788777E-4	0.0	0.015053696250493506	0.0014201600236314628	0.0
12	8.520960141788777E-4	0.0	0.01562176025994609	0.0014201600236314628	0.0
13	8.520960141788777E-4	0.0	0.016473856274124968	0.0014201600236314628	0.0
14	8.520960141788777E-4	0.0	0.018178048302482725	0.0014201600236314628	0.0
15	8.520960141788777E-4	0.0	0.019030144316661602	0.0017041920283577554	0.0
16	8.520960141788777E-4	0.0	0.021018368349745648	0.0017041920283577554	0.0
17	8.520960141788777E-4	0.0	0.021586432359198233	0.0017041920283577554	0.0
18	8.520960141788777E-4	0.0	0.02215449636865082	0.0017041920283577554	0.0
19	0.0011361280189051703	0.0	0.022438528373377113	0.0017041920283577554	0.0
20	0.0011361280189051703	0.0	0.023574656392282282	0.0017041920283577554	0.0
21	0.0011361280189051703	0.0	0.02442675240646116	0.0017041920283577554	0.0
22	0.0011361280189051703	0.0	0.02471078441118745	0.001988224033084048	0.0
23	0.0011361280189051703	0.0	0.02471078441118745	0.0022722560378103406	0.0
24	0.0011361280189051703	0.0	0.02471078441118745	0.003124352051989218	0.0
25	0.0011361280189051703	0.0	0.02471078441118745	0.003124352051989218	0.0
26	0.0011361280189051703	0.0	0.02471078441118745	0.003692416061441803	0.0
27	0.0011361280189051703	0.0	0.02471078441118745	0.005964672099252144	0.0
28	0.0011361280189051703	0.0	0.02471078441118745	0.011361280189051703	0.0
29	0.0011361280189051703	0.0	0.02471078441118745	0.022438528373377113	0.0
30	0.0011361280189051703	0.0	0.02471078441118745	0.04629721677038569	0.0
31	0.0011361280189051703	0.0	0.02471078441118745	0.11219264186688556	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCTAAA	30	3.594453E-4	30.833332	11
AGGTCGC	50	2.711713E-7	29.6	35
GGTATCA	230	0.0	27.347826	1
GGTCGCC	55	6.2410254E-7	26.90909	36
ATACTTA	35	8.8571274E-4	26.42857	6
GGCCTAA	35	8.8571274E-4	26.42857	10
CTTAGGT	50	9.067715E-6	25.900002	30
CGAACCG	120	0.0	24.666666	31
GCCGGCA	45	1.3206666E-4	24.666666	15
GGCGAAC	125	0.0	23.68	29
TAGCTCG	55	1.8978022E-5	23.545454	36
CCGGGGG	150	0.0	23.433334	35
GCGAACC	130	0.0	22.76923	30
TGCTCGG	115	5.456968E-12	22.52174	10
GTATCAA	580	0.0	22.327585	1
GCTCCGA	75	3.7255268E-7	22.2	29
GAATCCA	135	0.0	21.925924	11
AACCGGG	145	0.0	21.689655	33
TCCGAGG	80	6.931932E-7	20.8125	31
CATAGAC	80	6.931932E-7	20.8125	2
>>END_MODULE