FastQCFastQC Report
Fri 10 Feb 2017
ERR1632783.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632783.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433781
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12370.2851669390775529No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9120.2102443398857949No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5070.11687925473914257No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT4340.10005048630530153No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCCG250.005492619229.59999819
TAATCGC250.005492619229.59999832
GGTATCA2550.026.1176471
GTATCAA6950.023.1582741
GTCGCCA400.001929259923.12512
ATTTCGG400.001929259923.12521
GTATTAG1500.022.21
AGCTTCG603.7194048E-521.58333221
AAACGTC603.7194048E-521.58333215
AATTTCG450.00382202620.55555520
TTCTGCG450.00382202620.55555518
TCACGAT656.889474E-519.92307720
TCGCCGC656.889474E-519.92307725
CAAACGT656.889474E-519.92307714
CCGCTCT656.889474E-519.92307728
GCCGCTC656.889474E-519.92307727
GCTTCGC656.889474E-519.92307722
GTCACGA656.889474E-519.92307719
CGAATCT656.889474E-519.92307734
TGCTCGG656.889474E-519.92307710
Produced by FastQC (version 0.11.5)