##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632783.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	433781
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.694986640724235	33.0	33.0	33.0	27.0	33.0
2	31.23184740687121	33.0	33.0	33.0	27.0	33.0
3	31.667541916312608	33.0	33.0	33.0	27.0	33.0
4	31.79190420972795	33.0	33.0	33.0	27.0	33.0
5	31.913161249570635	33.0	33.0	33.0	33.0	33.0
6	34.78828948248079	37.0	37.0	37.0	27.0	37.0
7	34.90874657949518	37.0	37.0	37.0	27.0	37.0
8	35.12472422720221	37.0	37.0	37.0	33.0	37.0
9	35.168186250665656	37.0	37.0	37.0	33.0	37.0
10	35.247369064112995	37.0	37.0	37.0	33.0	37.0
11	35.28352094720608	37.0	37.0	37.0	33.0	37.0
12	35.17996177794786	37.0	37.0	37.0	33.0	37.0
13	35.243848854606355	37.0	37.0	37.0	33.0	37.0
14	35.19503620490524	37.0	37.0	37.0	33.0	37.0
15	35.25387234572284	37.0	37.0	37.0	33.0	37.0
16	35.243237947259104	37.0	37.0	37.0	33.0	37.0
17	35.21143618554063	37.0	37.0	37.0	33.0	37.0
18	35.188717348154945	37.0	37.0	37.0	33.0	37.0
19	35.22375807146924	37.0	37.0	37.0	33.0	37.0
20	35.170735924348925	37.0	37.0	37.0	33.0	37.0
21	35.22000963619891	37.0	37.0	37.0	33.0	37.0
22	35.22963891917811	37.0	37.0	37.0	33.0	37.0
23	35.20945592361122	37.0	37.0	37.0	33.0	37.0
24	35.190393309066096	37.0	37.0	37.0	33.0	37.0
25	35.21655167008237	37.0	37.0	37.0	33.0	37.0
26	35.17562779374846	37.0	37.0	37.0	33.0	37.0
27	35.116406666036546	37.0	37.0	37.0	33.0	37.0
28	35.09534995769755	37.0	37.0	37.0	33.0	37.0
29	35.139164693704885	37.0	37.0	37.0	33.0	37.0
30	35.098413715676806	37.0	37.0	37.0	33.0	37.0
31	35.08301193459372	37.0	37.0	37.0	33.0	37.0
32	35.12741452484087	37.0	37.0	37.0	33.0	37.0
33	35.08416228465516	37.0	37.0	37.0	33.0	37.0
34	35.038521742538286	37.0	37.0	37.0	27.0	37.0
35	35.04665487884439	37.0	37.0	37.0	27.0	37.0
36	35.01852317183095	37.0	37.0	37.0	27.0	37.0
37	35.04922991094585	37.0	37.0	37.0	27.0	37.0
38	34.90020309787658	37.0	37.0	37.0	27.0	37.0
39	34.96568314425943	37.0	37.0	37.0	27.0	37.0
40	35.013283200509015	37.0	37.0	37.0	27.0	37.0
41	35.037618060726494	37.0	37.0	37.0	27.0	37.0
42	34.903414395743475	37.0	37.0	37.0	27.0	37.0
43	34.71341298950392	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	10.0
16	21.0
17	19.0
18	22.0
19	21.0
20	31.0
21	95.0
22	259.0
23	635.0
24	1307.0
25	2236.0
26	3568.0
27	5321.0
28	7754.0
29	10321.0
30	13427.0
31	17050.0
32	21853.0
33	28617.0
34	40552.0
35	74029.0
36	206629.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.96675511375556	20.33307129634539	13.865291471963964	24.834882117935088
2	15.784001604496279	23.45077354702027	38.137216706125905	22.62800814235755
3	18.012776032145254	26.59452580910644	30.715729826802	24.676968331946306
4	13.085174316071935	18.067411896786627	38.86062321770663	29.986790569434806
5	13.932837076773763	37.056717560243534	35.51354254796775	13.49690281501495
6	29.434668646160162	39.25206498209926	17.935087060060262	13.378179311680318
7	26.31466108474092	32.24322872601612	23.710351536835407	17.73175865240755
8	24.029176012780642	36.131826889605584	21.24528275788935	18.593714339724425
9	25.866739207111422	15.775241423667705	20.13527563447915	38.22274373474173
10	14.81992065120418	28.42286776045977	34.168163197558215	22.589048390777837
11	33.382743826954155	23.886246746630214	24.42937795800185	18.301631468413785
12	22.286361090043133	25.486593465366163	30.78465861805842	21.442386826532285
13	27.93068391653853	22.061593292467858	26.847648928837366	23.16007386215625
14	22.197606626385202	21.03895744626897	27.63975370060007	29.123682226745757
15	23.72718030526925	28.622968733070376	24.48401382264322	23.165837139017153
16	23.07316364709381	28.510930630894393	25.0548087629472	23.361096959064596
17	22.420530175364988	27.76792897798659	26.508768249416182	23.302772597232245
18	22.983487059138135	27.515727982553408	27.423054490630065	22.077730467678393
19	24.695410817901198	26.466581062794358	27.414063778727055	21.42394434057739
20	24.458424873380807	26.142684903211528	27.405995191121786	21.99289503228588
21	22.902801183085476	27.172006150569068	26.886147618268204	23.039045048077256
22	22.618556368305665	26.970060929362973	26.980434827712603	23.43094787461876
23	22.58858732862896	27.1021552350149	27.56644481892937	22.742812617426765
24	23.113276054045706	26.9154250647216	26.882459121077222	23.08883976015547
25	23.35371996468264	26.820907324202764	27.349515077884924	22.47585763322967
26	23.17874687918558	27.06665344955173	27.015475551026903	22.739124120235786
27	23.322598269633755	26.850645832805032	26.589684656543277	23.237071241017933
28	22.734282967672627	27.049594150043454	27.333147371599953	22.882975510683963
29	23.41896025874808	27.06434813880737	26.80638386651329	22.710307735931266
30	23.05495169221335	27.315627009942805	27.06734504277504	22.562076255068803
31	23.461839038593208	26.781486510474178	26.970060929362973	22.78661352156964
32	22.53072402894548	26.78125597939974	27.408761564015023	23.279258427639753
33	22.681952413775615	26.70979134632453	27.76700685368884	22.841249386211015
34	23.01345609881484	26.471652746431957	27.196903506608173	23.31798764814503
35	22.977954313351667	27.183532704290876	27.28173894200069	22.55677404035677
36	23.187045997865283	26.80730599081103	27.07633575467805	22.929312256645634
37	23.625516101442894	26.40364607947328	26.896982578766703	23.07385524031712
38	23.545752349687977	26.615734667954566	26.954154285226878	22.88435869713058
39	22.968041477150912	26.62242006911322	27.27436194761873	23.13517650611714
40	22.818887871990707	26.86609141479226	27.520799666191003	22.794221047026035
41	22.780850244708738	26.43407618129886	27.582351463065464	23.202722110926942
42	23.005387511209573	26.80062058965238	27.024927325078785	23.169064574059263
43	22.95490120590805	26.248959728526604	27.66764796060685	23.128491104958492
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	13.0
2	18.0
3	77.0
4	136.0
5	136.0
6	175.5
7	215.0
8	209.0
9	203.0
10	262.0
11	321.0
12	321.0
13	514.0
14	707.0
15	1272.5
16	1838.0
17	2160.0
18	2482.0
19	2482.0
20	2504.5
21	2527.0
22	3065.5
23	3604.0
24	4760.5
25	5917.0
26	5917.0
27	7449.0
28	8981.0
29	11493.0
30	14005.0
31	15994.0
32	17983.0
33	17983.0
34	20312.0
35	22641.0
36	24383.5
37	26126.0
38	28612.5
39	31099.0
40	31099.0
41	32297.5
42	33496.0
43	33065.0
44	32634.0
45	33050.5
46	33467.0
47	33467.0
48	32852.5
49	32238.0
50	32889.5
51	33541.0
52	33284.0
53	33027.0
54	33027.0
55	29464.5
56	25902.0
57	23331.0
58	20760.0
59	18051.5
60	15343.0
61	15343.0
62	13583.0
63	11823.0
64	9808.0
65	7793.0
66	6702.0
67	5611.0
68	5611.0
69	4524.5
70	3438.0
71	2953.5
72	2469.0
73	2018.5
74	1568.0
75	1568.0
76	1192.0
77	816.0
78	633.0
79	450.0
80	363.5
81	277.0
82	277.0
83	218.5
84	160.0
85	129.0
86	98.0
87	67.5
88	37.0
89	37.0
90	28.0
91	19.0
92	11.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	433781.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.89960090997403
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.10300639333056	57.827495548060185
2	6.0645811173817785	7.871777884084836
3	1.6240889074752185	3.1620816581237228
4	0.7637000370103066	1.9825531046760523
5	0.4382794334792916	1.4222080159927768
6	0.29253652758038234	1.1391302334933855
7	0.22813303016550424	1.0364019828487003
8	0.16938035759470027	0.879417408790779
9	0.12953598525947158	0.7566150372137986
>10	1.0280325716108054	13.488842966924683
>50	0.10974948471267404	4.919997675659923
>100	0.04790368386509207	4.897260723911843
>500	7.149803561954039E-4	0.3292217627075471
>1k	3.5749017809770197E-4	0.28699599751179405
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1237	0.2851669390775529	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	912	0.2102443398857949	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	507	0.11687925473914257	No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT	434	0.10005048630530153	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.3053107443617862E-4	0.0	0.0	0.0	0.0
3	2.3053107443617862E-4	0.0	2.3053107443617862E-4	0.0	0.0
4	2.3053107443617862E-4	0.0	2.3053107443617862E-4	4.6106214887235724E-4	0.0
5	2.3053107443617862E-4	0.0	4.6106214887235724E-4	4.6106214887235724E-4	0.0
6	2.3053107443617862E-4	0.0	0.0020747796699256077	4.6106214887235724E-4	0.0
7	2.3053107443617862E-4	0.0	0.005532745786468287	4.6106214887235724E-4	0.0
8	2.3053107443617862E-4	0.0	0.006454870084213002	4.6106214887235724E-4	0.0
9	2.3053107443617862E-4	0.0	0.008299118679702431	9.221242977447145E-4	0.0
10	2.3053107443617862E-4	0.0	0.00852964975413861	9.221242977447145E-4	0.0
11	2.3053107443617862E-4	0.0	0.012679209093989825	9.221242977447145E-4	0.0
12	2.3053107443617862E-4	0.0	0.014292926615043075	9.221242977447145E-4	0.0
13	2.3053107443617862E-4	0.0	0.015445581987223967	9.221242977447145E-4	0.0
14	2.3053107443617862E-4	0.0	0.017520361657149576	0.0011526553721808931	0.0
15	2.3053107443617862E-4	0.0	0.018903548103766647	0.0011526553721808931	0.0
16	2.3053107443617862E-4	0.0	0.022130983145873147	0.0011526553721808931	0.0
17	2.3053107443617862E-4	0.0	0.022592045294745504	0.0011526553721808931	0.0
18	2.3053107443617862E-4	0.0	0.023514169592490218	0.0011526553721808931	0.0
19	2.3053107443617862E-4	0.0	0.0237447006669264	0.0013831864466170717	0.0
20	2.3053107443617862E-4	0.0	0.024205762815798757	0.0013831864466170717	0.0
21	2.3053107443617862E-4	0.0	0.02512788711354347	0.0016137175210532504	0.0
22	2.3053107443617862E-4	0.0	0.02558894926241583	0.0020747796699256077	0.0
23	2.3053107443617862E-4	0.0	0.026050011411288185	0.0023053107443617863	0.0
24	2.3053107443617862E-4	0.0	0.026050011411288185	0.0023053107443617863	0.0
25	2.3053107443617862E-4	0.0	0.026050011411288185	0.0023053107443617863	0.0
26	2.3053107443617862E-4	0.0	0.026280542485724364	0.0027663728932341434	0.0
27	2.3053107443617862E-4	0.0	0.026280542485724364	0.00507168363759593	0.0
28	2.3053107443617862E-4	0.0	0.026511073560160543	0.012218146945117468	0.0
29	2.3053107443617862E-4	0.0	0.026511073560160543	0.028816384304522328	0.0
30	2.3053107443617862E-4	0.0	0.026511073560160543	0.05117789852483166	0.0
31	2.3053107443617862E-4	0.0	0.026511073560160543	0.11042438465492956	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGCCG	25	0.0054926192	29.599998	19
TAATCGC	25	0.0054926192	29.599998	32
GGTATCA	255	0.0	26.117647	1
GTATCAA	695	0.0	23.158274	1
GTCGCCA	40	0.0019292599	23.125	12
ATTTCGG	40	0.0019292599	23.125	21
GTATTAG	150	0.0	22.2	1
AGCTTCG	60	3.7194048E-5	21.583332	21
AAACGTC	60	3.7194048E-5	21.583332	15
AATTTCG	45	0.003822026	20.555555	20
TTCTGCG	45	0.003822026	20.555555	18
TCACGAT	65	6.889474E-5	19.923077	20
TCGCCGC	65	6.889474E-5	19.923077	25
CAAACGT	65	6.889474E-5	19.923077	14
CCGCTCT	65	6.889474E-5	19.923077	28
GCCGCTC	65	6.889474E-5	19.923077	27
GCTTCGC	65	6.889474E-5	19.923077	22
GTCACGA	65	6.889474E-5	19.923077	19
CGAATCT	65	6.889474E-5	19.923077	34
TGCTCGG	65	6.889474E-5	19.923077	10
>>END_MODULE