FastQCFastQC Report
Fri 10 Feb 2017
ERR1632782.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632782.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164856
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4160.25234143737564907No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2980.1807638181200563No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1870.11343232882030378No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTACC250.005482865529.59999826
TAGCACC250.005482865529.5999984
ACATATA250.005482865529.5999981
GGTATCA1300.027.0384621
ATCCTAC358.837071E-426.42857225
CGAACCG358.837071E-426.42857231
GCGAACC358.837071E-426.42857230
GGCGAAC358.837071E-426.42857229
CAATACA358.837071E-426.4285724
GTATCAA3900.025.6153851
ACCGGGG451.3165067E-424.66666834
TACCACT400.001924215923.12529
ATACACA400.001924215923.1254
TGTTCTC502.6885213E-422.1999995
CCGGGGG450.00381212920.55555735
TAATATA555.1174534E-420.1818184
TACAATA656.856404E-519.9230772
ATCAACG5200.019.2115383
CAACGCA5300.018.8490545
AACGCAG5300.018.8490546
Produced by FastQC (version 0.11.5)