##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632782.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	164856
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.339132333673025	33.0	33.0	33.0	14.0	33.0
2	30.290847770175183	33.0	33.0	33.0	14.0	33.0
3	30.75644805163294	33.0	33.0	33.0	27.0	33.0
4	30.931898141408258	33.0	33.0	33.0	27.0	33.0
5	31.049067064589703	33.0	33.0	33.0	27.0	33.0
6	33.24020357160188	37.0	33.0	37.0	27.0	37.0
7	33.45422065317611	37.0	33.0	37.0	27.0	37.0
8	33.65343693890426	37.0	33.0	37.0	27.0	37.0
9	33.59592007570243	37.0	33.0	37.0	27.0	37.0
10	33.67489202698112	37.0	33.0	37.0	27.0	37.0
11	33.696929441451935	37.0	33.0	37.0	27.0	37.0
12	33.7027405735915	37.0	33.0	37.0	27.0	37.0
13	33.786613772019216	37.0	33.0	37.0	27.0	37.0
14	33.76573494443635	37.0	33.0	37.0	27.0	37.0
15	33.85403624981802	37.0	33.0	37.0	27.0	37.0
16	33.80058960547387	37.0	33.0	37.0	27.0	37.0
17	33.814595768428205	37.0	33.0	37.0	27.0	37.0
18	33.807571456301254	37.0	33.0	37.0	27.0	37.0
19	33.804417188334064	37.0	33.0	37.0	27.0	37.0
20	33.692895617993884	37.0	33.0	37.0	27.0	37.0
21	33.776538312224	37.0	33.0	37.0	27.0	37.0
22	33.76279298296695	37.0	33.0	37.0	27.0	37.0
23	33.75566555054108	37.0	33.0	37.0	27.0	37.0
24	33.76633546853011	37.0	33.0	37.0	27.0	37.0
25	33.73906924831368	37.0	33.0	37.0	27.0	37.0
26	33.67814334934731	37.0	33.0	37.0	27.0	37.0
27	33.55844494589217	37.0	33.0	37.0	27.0	37.0
28	33.57551438831465	37.0	33.0	37.0	27.0	37.0
29	33.60269568593196	37.0	33.0	37.0	27.0	37.0
30	33.59261416023681	37.0	33.0	37.0	27.0	37.0
31	33.562308924151985	37.0	33.0	37.0	27.0	37.0
32	33.60383607512011	37.0	33.0	37.0	27.0	37.0
33	33.56985490367351	37.0	33.0	37.0	27.0	37.0
34	33.48489590915708	37.0	33.0	37.0	27.0	37.0
35	33.48848692191974	37.0	33.0	37.0	27.0	37.0
36	33.458618430630366	37.0	33.0	37.0	27.0	37.0
37	33.48083782209929	37.0	33.0	37.0	27.0	37.0
38	33.35689328868831	37.0	33.0	37.0	22.0	37.0
39	33.420840005823266	37.0	33.0	37.0	27.0	37.0
40	33.4423557528995	37.0	33.0	37.0	27.0	37.0
41	33.475232930557574	37.0	33.0	37.0	27.0	37.0
42	33.31008880477508	37.0	33.0	37.0	14.0	37.0
43	33.038093851603826	37.0	33.0	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	6.0
16	13.0
17	15.0
18	15.0
19	19.0
20	85.0
21	195.0
22	403.0
23	911.0
24	1703.0
25	2543.0
26	3544.0
27	4687.0
28	6183.0
29	7427.0
30	9052.0
31	10796.0
32	12914.0
33	15813.0
34	20824.0
35	30404.0
36	37304.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.22446256126559	21.025015771339834	12.718372397728928	25.03214926966565
2	16.7285388460232	24.442543795797544	35.65839277915271	23.170524579026544
3	18.92621439316737	27.294123356141114	29.61554326199835	24.16411898869316
4	14.4471538797496	19.64320376571068	37.295579172125976	28.61406318241374
5	15.645169117290243	36.570704129664676	34.48828068132188	13.295846071723199
6	29.63131460183433	39.48536904935216	17.498908138011355	13.384408210802153
7	26.877396030475083	32.365822293395446	22.947299461348088	17.809482214781386
8	24.664555733488623	35.95258892609308	20.60768185568011	18.775173484738193
9	26.169505507837144	15.648202067258698	20.04537293152812	38.136919493376034
10	15.102877662930073	28.328965885378754	33.71366525937788	22.854491192313294
11	34.132818945018684	23.596957344591644	23.891760081525696	18.37846362886398
12	23.142014849323044	25.16256611830931	30.518755762604936	21.176663269762702
13	28.12818459746688	22.010724511088466	26.415174455282187	23.44591643616247
14	22.395302567088855	20.927961372349202	27.57618770320765	29.100548357354295
15	24.024603290144125	28.540059203183382	24.037948270005337	23.397389236667152
16	23.30518755762605	28.159120687145144	25.168025428252534	23.36766632697627
17	22.470519726306595	27.807298490804094	26.301742126461882	23.420439656427426
18	23.46290095598583	27.08545639831125	27.28866404619789	22.162978599505024
19	24.712476342990247	26.58987237346533	26.900446450235354	21.79720483330907
20	24.59843742417625	26.133110108215657	26.931989129907315	22.33646333770078
21	23.444703256175085	26.713010142184697	26.83068860096084	23.01159800067938
22	23.18508273887514	26.813097491143782	26.80703159120687	23.194788178774203
23	23.012204590673072	26.61474256320668	27.47913330421701	22.893919541903237
24	23.336123647304312	26.803392051244725	26.559542873780757	23.30094142767021
25	23.433178046294948	26.628694133061583	27.125491337894893	22.81263648274858
26	23.627286844276217	26.928956179938858	26.617168923181445	22.826588052603483
27	23.542364245159412	26.9259232299704	26.359974765856265	23.171737759013926
28	23.26697238802349	26.57531421361673	26.92531663997671	23.23239675838307
29	23.451982336099384	26.989008589314313	26.643252292910173	22.91575678167613
30	23.564201484932305	26.64810501285971	27.269859756393462	22.517833745814528
31	23.764376182850487	26.4139612752948	26.825835881011308	22.995826660843402
32	22.52268646576406	26.730601252001744	27.061799388557283	23.684912893676906
33	22.905444751783374	26.87133013053817	27.359635075459792	22.86359004221866
34	23.416193526471588	26.314480516329404	27.036322608822243	23.233003348376766
35	23.394356286698695	26.964138399572963	26.99082835929538	22.65067695443296
36	23.69037220362013	26.551050613869076	26.898020090260594	22.860557092250204
37	23.394356286698695	26.400616295433593	27.026617168923185	23.178410248944534
38	23.613335274421313	26.508589314310672	26.66084340272723	23.217232008540787
39	22.90665793177076	26.62990731304896	27.104260688115687	23.35917406706459
40	22.95700490124715	27.059373028582524	27.260760906488084	22.722861163682243
41	22.992187120881255	26.107633328480613	27.27167952637453	23.628500024263598
42	23.151113699228418	26.729994662008057	26.777308681515986	23.341582957247535
43	23.412553986509437	26.107026738486923	27.15764060756054	23.322778667443103
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	2.0
2	4.0
3	23.0
4	42.0
5	42.0
6	52.5
7	63.0
8	62.0
9	61.0
10	105.5
11	150.0
12	150.0
13	197.0
14	244.0
15	419.0
16	594.0
17	701.0
18	808.0
19	808.0
20	827.0
21	846.0
22	950.5
23	1055.0
24	1485.5
25	1916.0
26	1916.0
27	2499.0
28	3082.0
29	3785.0
30	4488.0
31	5554.5
32	6621.0
33	6621.0
34	7571.5
35	8522.0
36	9392.0
37	10262.0
38	10945.0
39	11628.0
40	11628.0
41	12062.0
42	12496.0
43	12761.0
44	13026.0
45	12936.0
46	12846.0
47	12846.0
48	12775.0
49	12704.0
50	12627.0
51	12550.0
52	12299.0
53	12048.0
54	12048.0
55	11174.5
56	10301.0
57	9261.5
58	8222.0
59	7277.5
60	6333.0
61	6333.0
62	5569.5
63	4806.0
64	3987.0
65	3168.0
66	2667.0
67	2166.0
68	2166.0
69	1778.0
70	1390.0
71	1204.5
72	1019.0
73	821.0
74	623.0
75	623.0
76	474.5
77	326.0
78	264.5
79	203.0
80	155.5
81	108.0
82	108.0
83	86.5
84	65.0
85	52.0
86	39.0
87	29.5
88	20.0
89	20.0
90	15.0
91	10.0
92	5.0
93	0.0
94	0.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	164856.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.79649828189508
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.16336306213486	64.01617242898553
2	6.14191493918051	8.819359707568385
3	1.6203997006728352	3.4901707297602154
4	0.794356253679804	2.281279896101386
5	0.47273176168676834	1.697024255787065
6	0.31303052923992897	1.3484697512853159
7	0.22727528696383711	1.1422298825011439
8	0.18839544305189823	1.082090648271398
9	0.14852272747036696	0.9597070572883716
>10	0.8711993577340016	11.60592836302707
>50	0.05183336924797108	2.572813190410318
>100	0.006977568937226876	0.9847540890137964
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	416	0.25234143737564907	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	298	0.1807638181200563	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	187	0.11343232882030378	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0012131799873829282	0.0	0.0
2	0.0	0.0	0.0012131799873829282	0.0	0.0
3	0.0	0.0	0.0012131799873829282	0.0	0.0
4	0.0	0.0	0.0012131799873829282	0.0	0.0
5	0.0	0.0	0.0012131799873829282	0.0	0.0
6	0.0	0.0	0.0012131799873829282	0.0	0.0
7	0.0	0.0	0.0054593099432231765	0.0	0.0
8	0.0	0.0	0.007279079924297569	0.0	0.0
9	0.0	0.0	0.007885669917989033	6.065899936914641E-4	0.0
10	0.0	0.0	0.00909884990537196	6.065899936914641E-4	0.0
11	0.0	0.0	0.013951569854903674	0.0012131799873829282	0.0
12	0.0	0.0	0.01637792982966953	0.0012131799873829282	0.0
13	0.0	0.0	0.01637792982966953	0.0012131799873829282	0.0
14	0.0	0.0	0.017591109817052458	0.0012131799873829282	0.0
15	0.0	0.0	0.019410879798126852	0.0012131799873829282	0.0
16	0.0	0.0	0.023050419760275633	0.0012131799873829282	0.0
17	0.0	0.0	0.0236570097539671	0.0012131799873829282	0.0
18	0.0	0.0	0.024263599747658564	0.0012131799873829282	0.0
19	0.0	0.0	0.024870189741350027	0.0012131799873829282	0.0
20	0.0	0.0	0.026083369728732955	0.0012131799873829282	0.0
21	0.0	0.0	0.026083369728732955	0.0012131799873829282	0.0
22	0.0	0.0	0.026083369728732955	0.0018197699810743922	0.0
23	0.0	0.0	0.026083369728732955	0.0018197699810743922	0.0
24	0.0	0.0	0.026689959722424418	0.0018197699810743922	0.0
25	0.0	0.0	0.026689959722424418	0.0024263599747658565	0.0
26	0.0	0.0	0.026689959722424418	0.0024263599747658565	0.0
27	0.0	0.0	0.026689959722424418	0.0024263599747658565	0.0
28	0.0	0.0	0.026689959722424418	0.012131799873829282	0.0
29	0.0	0.0	0.026689959722424418	0.023050419760275633	0.0
30	0.0	0.0	0.026689959722424418	0.04003493958363663	0.0
31	0.0	0.0	0.026689959722424418	0.09038190906002815	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCTACC	25	0.0054828655	29.599998	26
TAGCACC	25	0.0054828655	29.599998	4
ACATATA	25	0.0054828655	29.599998	1
GGTATCA	130	0.0	27.038462	1
ATCCTAC	35	8.837071E-4	26.428572	25
CGAACCG	35	8.837071E-4	26.428572	31
GCGAACC	35	8.837071E-4	26.428572	30
GGCGAAC	35	8.837071E-4	26.428572	29
CAATACA	35	8.837071E-4	26.428572	4
GTATCAA	390	0.0	25.615385	1
ACCGGGG	45	1.3165067E-4	24.666668	34
TACCACT	40	0.0019242159	23.125	29
ATACACA	40	0.0019242159	23.125	4
TGTTCTC	50	2.6885213E-4	22.199999	5
CCGGGGG	45	0.003812129	20.555557	35
TAATATA	55	5.1174534E-4	20.181818	4
TACAATA	65	6.856404E-5	19.923077	2
ATCAACG	520	0.0	19.211538	3
CAACGCA	530	0.0	18.849054	5
AACGCAG	530	0.0	18.849054	6
>>END_MODULE