FastQCFastQC Report
Fri 10 Feb 2017
ERR1632781.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632781.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106882
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25432.379259370146517No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21822.0415037143766024No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12041.126475926722928No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA6830.6390224733818604No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5820.5445257386650698No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5010.46874122864467355No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4410.4126045545554911No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA4090.38266499504126045No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG3960.3705020489886043No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG3770.3527254355270298No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3660.342433711944013No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC3600.3368200445350948No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3580.33494882206545534No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC3350.31342976366460207No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3150.29471753896820796No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC3030.28349020415037146No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3020.28255459291555174No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC2770.259164312045059No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT2730.25542186710578024No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2700.2526150334013211No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA2600.24325892105312402No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT2550.23858086487902547No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG2510.23483841993974663No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT2340.2189330289478116No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC2320.21706180647817222No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA2290.21425497277371305No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC2270.21238375030407367No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT2240.20957691659961453No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC2110.1974139705469583No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG2090.19554274807731892No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG2070.1936715256076795No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC2030.18992908066840067No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA1980.18525102449430214No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1980.18525102449430214No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC1920.1796373570853839No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1880.17589491214610506No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC1850.17308807844164592No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT1840.17215246720682623No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA1820.1702812447371868No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG1770.16560318856308828No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG1750.16373196609344884No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG1750.16373196609344884No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1700.1590539099193503No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT1680.1571826874497109No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1680.1571826874497109No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1640.15344024251043206No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC1640.15344024251043206No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG1620.15156902004079265No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT1620.15156902004079265No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC1590.1487621863363335No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC1590.1487621863363335No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA1580.1478265751015138No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1580.1478265751015138No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT1580.1478265751015138No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT1570.14689096386669412No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC1550.1450197413970547No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG1550.1450197413970547No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC1510.14127729645777587No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG1490.13940607398813645No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG1470.13753485151849704No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG1470.13753485151849704No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC1450.13566362904885762No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1440.13472801781403793No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT1440.13472801781403793No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA1430.1337924065792182No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA1410.1319211841095788No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1410.1319211841095788No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT1400.1309855728747591No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA1390.13004996163993937No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG1380.12911435040511968No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG1360.12724312793548023No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1340.12537190546584084No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG1330.12443629423102114No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA1320.12350068299620141No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1320.12350068299620141No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG1320.12350068299620141No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG1310.1225650717613817No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT1290.12069384929174229No Hit
GAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACC1260.11788701558728316No Hit
GGTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAA1230.11508018188282405No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG1210.11320895941318464No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC1210.11320895941318464No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG1200.11227334817836493No Hit
AATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAAC1200.11227334817836493No Hit
GAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAA1170.1094665144739058No Hit
GCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTAC1170.1094665144739058No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA1160.10853090323908611No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG1140.10665968076944668No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1130.10572406953462699No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG1120.10478845829980726No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG1110.10385284706498756No Hit
GTATCAACGCAGAGTACGGGGGTTAAGCGACTAAGCGTACACG1100.10291723583016786No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG1090.10198162459534812No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG1090.10198162459534812No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG1080.10104601336052843No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA1080.10104601336052843No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTAC200.00183447537.015
CGTTATT200.00183447537.010
TTATTGT200.00183447537.012
TATTGTT200.00183447537.013
CTTAGGA308.241577E-637.02
ATTGTTA200.00183447537.014
GAATCAA200.00183447537.01
TTAGGAC251.2261466E-437.03
CGGCCGT200.00183447537.014
GGACCGT401.582157E-632.3756
TAGGACC303.5778605E-430.8333344
TTACCGG303.5778605E-430.83333431
AGGACCG303.5778605E-430.8333345
CCGTTTA303.5778605E-430.83333427
TACCGGG303.5778605E-430.83333432
GACCGTT303.5778605E-430.8333347
GCCGTTT303.5778605E-430.83333426
CGCCGTT303.5778605E-430.83333425
GGCCGTA250.00547433529.615
TGTTACG250.00547433529.616
Produced by FastQC (version 0.11.5)