FastQCFastQC Report
Fri 10 Feb 2017
ERR1632780.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632780.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413963
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20330.49110669311025384No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13810.33360469413933125No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG12780.30872324338165485No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA10070.24325845546582667No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG9100.21982640960665567No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG8920.21547819491113943No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7700.1860069619748625No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC7050.1703050755743871No Hit
GATACGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG7010.16933880564205014No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT6960.16813096822662896No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC6880.16619842836195506No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT6280.15170437937690082No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA6220.15025497447839542No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT5780.13962600522268898No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC5680.1372103303918466No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG5540.13382838562866728No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA5350.1292386034500668No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5150.12440725378838205No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4920.1188512016774446No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA4850.11716022929585494No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG4810.11619395936351799No Hit
ATGCAAGGGTCTCGCTGAAGACTTGGAGGATTAGGGAAGAGAG4770.11522768943118104No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC4760.11498612194809682No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA4720.11401985201575987No Hit
AGGGAAGAGAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTG4380.1058065575908958No Hit
CATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCC4230.10218304534463224No Hit
CAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAG4140.10000893799687412No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAC250.005492331529.635
TCGAACG250.005492331529.636
AGTCGAA250.005492331529.634
GGTATCA3950.029.0379731
GTATCAA11550.025.307361
TAGGGGT551.8988487E-523.5454544
CGAGTCG400.001929111723.12521
TTATACT753.728819E-722.24
CGGGCCC502.6980566E-422.210
CCGTTAA603.7188755E-521.58333416
GCGAACT957.1122486E-921.42105328
CGCTCTC909.4416464E-820.55555529
GAAGTGT450.003821735320.5555556
CGAACTA1001.2838427E-820.3529
CCGCTCT951.6696504E-719.47368428
GAGTCGC500.00702746518.522
ATACTGA801.612602E-518.56
AGAGTGT500.00702746518.56
GTGTCAA500.00702746518.528
GCCCCGT1258.53106E-917.7614
Produced by FastQC (version 0.11.5)