##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632780.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	413963
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.669081536272564	33.0	33.0	33.0	27.0	33.0
2	31.158627703442097	33.0	33.0	33.0	27.0	33.0
3	31.624604131287096	33.0	33.0	33.0	27.0	33.0
4	31.75599268533661	33.0	33.0	33.0	27.0	33.0
5	31.886250703565295	33.0	33.0	33.0	33.0	33.0
6	34.7342177924114	37.0	37.0	37.0	27.0	37.0
7	34.89057234583767	37.0	37.0	37.0	27.0	37.0
8	35.06356848317362	37.0	37.0	37.0	33.0	37.0
9	35.16519592330715	37.0	37.0	37.0	33.0	37.0
10	35.17438998171334	37.0	37.0	37.0	33.0	37.0
11	35.18584269608637	37.0	37.0	37.0	33.0	37.0
12	35.126477487118414	37.0	37.0	37.0	33.0	37.0
13	35.163355179086054	37.0	37.0	37.0	33.0	37.0
14	35.15970751009148	37.0	37.0	37.0	33.0	37.0
15	35.180612760077594	37.0	37.0	37.0	33.0	37.0
16	35.1737836473308	37.0	37.0	37.0	33.0	37.0
17	35.135253633778866	37.0	37.0	37.0	33.0	37.0
18	35.14198128818276	37.0	37.0	37.0	33.0	37.0
19	35.1426190263381	37.0	37.0	37.0	33.0	37.0
20	35.138836079553	37.0	37.0	37.0	33.0	37.0
21	35.13530677862514	37.0	37.0	37.0	33.0	37.0
22	35.138898887098605	37.0	37.0	37.0	33.0	37.0
23	35.10866913226545	37.0	37.0	37.0	33.0	37.0
24	35.113295149566504	37.0	37.0	37.0	33.0	37.0
25	35.115471672589095	37.0	37.0	37.0	33.0	37.0
26	35.077649451762596	37.0	37.0	37.0	33.0	37.0
27	35.01191893961538	37.0	37.0	37.0	27.0	37.0
28	34.987969939342406	37.0	37.0	37.0	27.0	37.0
29	35.024965999376754	37.0	37.0	37.0	27.0	37.0
30	35.00309447945831	37.0	37.0	37.0	27.0	37.0
31	34.97276326628225	37.0	37.0	37.0	27.0	37.0
32	35.0145761819293	37.0	37.0	37.0	27.0	37.0
33	35.001280307660345	37.0	37.0	37.0	27.0	37.0
34	34.92897915997323	37.0	37.0	37.0	27.0	37.0
35	34.89871075434278	37.0	37.0	37.0	27.0	37.0
36	34.887883699750944	37.0	37.0	37.0	27.0	37.0
37	34.9068346687989	37.0	37.0	37.0	27.0	37.0
38	34.76114773542563	37.0	37.0	37.0	27.0	37.0
39	34.834260549855905	37.0	37.0	37.0	27.0	37.0
40	34.86217125685146	37.0	37.0	37.0	27.0	37.0
41	34.885166065566246	37.0	37.0	37.0	27.0	37.0
42	34.78649299575083	37.0	37.0	37.0	27.0	37.0
43	34.58123310537415	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	11.0
16	13.0
17	17.0
18	14.0
19	25.0
20	43.0
21	100.0
22	288.0
23	657.0
24	1406.0
25	2279.0
26	3880.0
27	5731.0
28	7704.0
29	10786.0
30	13549.0
31	17025.0
32	21541.0
33	27562.0
34	38929.0
35	69452.0
36	192950.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.87372784524221	19.64257675202856	12.796554281421285	23.687141121307942
2	16.869140478738437	23.49992632191766	37.363001041155854	22.26793215818805
3	18.78138867483326	26.21369542688598	29.61303304884736	25.391882849433404
4	12.519959513289836	17.90304930633897	38.96797539876752	30.60901578160367
5	14.765087701074734	36.05056490555919	34.42433260943611	14.760014783929964
6	30.28918043400014	39.36801115075502	16.889673714800598	13.453134700444242
7	28.461963991950974	31.346038172493678	22.175894947132957	18.016102888422395
8	25.01890265555134	34.80021161311518	20.34433029038827	19.836555440945205
9	26.552373038170078	14.51796416587956	19.702485487833453	39.227177308116914
10	15.644151772018272	27.637252604701384	33.2505562091298	23.46803941415054
11	35.2289938955897	23.921461579899653	22.81918915458628	18.030355369924365
12	23.810340537680904	24.781441819679536	30.02176523022589	21.38645241241367
13	29.216137674139958	22.00776397890633	25.68804458369468	23.088053763259037
14	23.088536898225204	20.84365027792339	26.928976744298406	29.138836079553005
15	24.06277855750393	28.352292354630727	25.039194324130413	22.545734763734924
16	24.141046422023223	27.81118119252204	24.534076717001277	23.51369566845346
17	23.965668429304067	26.304766368008735	25.004167039083203	24.72539816360399
18	23.89561385920964	25.46845974157111	27.700301717786374	22.93562468143288
19	26.908201940753155	25.367001398675725	26.430139891729453	21.294656768841662
20	26.160550580607445	25.5788560813406	26.341243057954454	21.919350280097497
21	23.769998768005838	26.691757475909682	26.35863591673652	23.17960783934796
22	24.623215118259363	25.180269734251613	26.820996079359748	23.37551906812928
23	23.23951657515285	26.140017344545285	27.584107758422853	23.036358321879007
24	23.674821179670648	26.911825452999423	25.967779729106223	23.445573638223706
25	23.87121554341813	26.67074110488135	27.332636008532162	22.12540734316835
26	25.227133825969954	25.850619499810367	25.787087251759218	23.13515942246046
27	24.419815297502435	25.888787162137678	25.71872365404638	23.972673886313512
28	23.817829129656516	25.779115524817435	27.025120602565927	23.377934742960118
29	24.575867891574852	26.62146133833217	26.466616581675172	22.336054188417805
30	24.666697265214523	25.83805799068999	26.275295135072458	23.219949609023026
31	23.690764633554206	25.169882332478988	27.03816524665248	24.101187787314327
32	22.867985786169296	26.03372765198822	26.957240139819262	24.141046422023223
33	23.604041907126966	25.48005498075915	27.38819652964154	23.527706582472348
34	24.139838584607805	25.799165625913428	26.986711372755533	23.074284416723234
35	24.141046422023223	26.484975710389573	25.646978111570355	23.72699975601684
36	23.71443824689646	25.935409686372935	25.938791631136116	24.41136043559449
37	24.411843570560656	25.530542584723754	26.875590330536784	23.182023514178805
38	23.959870809710047	25.31337341743103	25.983240048023614	24.743515724835312
39	23.48180876068634	25.57982235127294	27.173684604662736	23.76468428337798
40	24.22172996137336	26.268772813029184	26.506475216384075	23.003022009213385
41	23.90310245118525	25.623787633194272	26.649241598886857	23.82386831673362
42	22.870401461000135	27.59159635039847	26.17770187190643	23.36030031669497
43	24.76453209586364	24.94981435538925	25.954251950053507	24.331401598693603
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9.0
1	20.0
2	31.0
3	115.5
4	200.0
5	200.0
6	271.5
7	343.0
8	312.0
9	281.0
10	363.0
11	445.0
12	445.0
13	689.5
14	934.0
15	1664.0
16	2394.0
17	2793.5
18	3193.0
19	3193.0
20	2798.5
21	2404.0
22	2695.0
23	2986.0
24	3798.5
25	4611.0
26	4611.0
27	5593.5
28	6576.0
29	8072.0
30	9568.0
31	11190.5
32	12813.0
33	12813.0
34	16781.0
35	20749.0
36	22274.5
37	23800.0
38	24883.5
39	25967.0
40	25967.0
41	26303.5
42	26640.0
43	28113.5
44	29587.0
45	29501.5
46	29416.0
47	29416.0
48	29072.5
49	28729.0
50	29858.0
51	30987.0
52	32365.0
53	33743.0
54	33743.0
55	32571.5
56	31400.0
57	26907.5
58	22415.0
59	20609.0
60	18803.0
61	18803.0
62	17291.0
63	15779.0
64	11990.0
65	8201.0
66	7073.5
67	5946.0
68	5946.0
69	5204.5
70	4463.0
71	4655.5
72	4848.0
73	4409.5
74	3971.0
75	3971.0
76	2342.0
77	713.0
78	563.5
79	414.0
80	317.5
81	221.0
82	221.0
83	192.0
84	163.0
85	141.0
86	119.0
87	86.5
88	54.0
89	54.0
90	45.5
91	37.0
92	22.0
93	7.0
94	5.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	413963.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.80917946317061
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.09388146070933	47.40259477126586
2	6.233746549441607	6.708655736136479
3	1.7296294089807658	2.7920981781787124
4	0.8557174983897254	1.8418182576251256
5	0.5448391709844143	1.4658674365032722
6	0.3360207533929639	1.084860061360307
7	0.2961263240697031	1.115402016094497
8	0.21899020584313575	0.9426946629511971
9	0.17376273303661927	0.8415027077380606
>10	1.2317003111869569	13.376063901902091
>50	0.1405262397287289	5.101397966698377
>100	0.13689975612279776	13.653325513432568
>500	0.006346346310328373	2.283603699159533
>1k	0.0018132418029509637	1.3901150909538753
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2033	0.49110669311025384	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1381	0.33360469413933125	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	1278	0.30872324338165485	No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA	1007	0.24325845546582667	No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG	910	0.21982640960665567	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	892	0.21547819491113943	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	770	0.1860069619748625	No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC	705	0.1703050755743871	No Hit
GATACGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	701	0.16933880564205014	No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT	696	0.16813096822662896	No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC	688	0.16619842836195506	No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT	628	0.15170437937690082	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	622	0.15025497447839542	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	578	0.13962600522268898	No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC	568	0.1372103303918466	No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG	554	0.13382838562866728	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	535	0.1292386034500668	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	515	0.12440725378838205	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	492	0.1188512016774446	No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA	485	0.11716022929585494	No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	481	0.11619395936351799	No Hit
ATGCAAGGGTCTCGCTGAAGACTTGGAGGATTAGGGAAGAGAG	477	0.11522768943118104	No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC	476	0.11498612194809682	No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA	472	0.11401985201575987	No Hit
AGGGAAGAGAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTG	438	0.1058065575908958	No Hit
CATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCC	423	0.10218304534463224	No Hit
CAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAG	414	0.10000893799687412	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.41567483084237E-4	0.0	0.0
2	0.0	0.0	2.41567483084237E-4	0.0	0.0
3	0.0	0.0	4.83134966168474E-4	0.0	0.0
4	0.0	0.0	4.83134966168474E-4	0.0	0.0
5	0.0	0.0	7.24702449252711E-4	0.0	0.0
6	0.0	0.0	0.001449404898505422	0.0	0.0
7	0.0	0.0	0.0048313496616847395	0.0	0.0
8	0.0	0.0	0.005797619594021688	0.0	0.0
9	0.0	0.0	0.00724702449252711	9.66269932336948E-4	0.0
10	0.0	0.0	0.008696429391032531	9.66269932336948E-4	0.0
11	2.41567483084237E-4	0.0	0.013286211569633034	9.66269932336948E-4	0.0
12	2.41567483084237E-4	0.0	0.014977183951222694	9.66269932336948E-4	0.0
13	2.41567483084237E-4	0.0	0.015701886400475405	9.66269932336948E-4	0.0
14	2.41567483084237E-4	0.0	0.0176344262651493	9.66269932336948E-4	0.0
15	2.41567483084237E-4	0.0	0.019083831163654724	0.0012078374154211849	0.0
16	2.41567483084237E-4	0.0	0.020533236062160145	0.0012078374154211849	0.0
17	2.41567483084237E-4	0.0	0.020774803545244382	0.0012078374154211849	0.0
18	2.41567483084237E-4	0.0	0.02101637102832862	0.0012078374154211849	0.0
19	2.41567483084237E-4	0.0	0.021499505994497094	0.001449404898505422	0.0
20	2.41567483084237E-4	0.0	0.02246577592683404	0.001690972381589659	0.0
21	2.41567483084237E-4	0.0	0.023190478376086752	0.001690972381589659	0.0
22	2.41567483084237E-4	0.0	0.02343204585917099	0.002174107347758133	0.0
23	2.41567483084237E-4	0.0	0.02343204585917099	0.002657242313926607	0.0
24	2.41567483084237E-4	0.0	0.02343204585917099	0.002898809797010844	0.0
25	2.41567483084237E-4	0.0	0.02343204585917099	0.002898809797010844	0.0
26	2.41567483084237E-4	0.0	0.023673613342255227	0.003381944763179318	0.0
27	2.41567483084237E-4	0.0	0.023673613342255227	0.005072917144768977	0.0
28	2.41567483084237E-4	0.0	0.023673613342255227	0.014010914018885746	0.0
29	2.41567483084237E-4	0.0	0.023673613342255227	0.029471232936276914	0.0
30	2.41567483084237E-4	0.0	0.023673613342255227	0.05773462845713264	0.0
31	2.41567483084237E-4	0.0	0.023673613342255227	0.12851390100081408	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAAC	25	0.0054923315	29.6	35
TCGAACG	25	0.0054923315	29.6	36
AGTCGAA	25	0.0054923315	29.6	34
GGTATCA	395	0.0	29.037973	1
GTATCAA	1155	0.0	25.30736	1
TAGGGGT	55	1.8988487E-5	23.545454	4
CGAGTCG	40	0.0019291117	23.125	21
TTATACT	75	3.728819E-7	22.2	4
CGGGCCC	50	2.6980566E-4	22.2	10
CCGTTAA	60	3.7188755E-5	21.583334	16
GCGAACT	95	7.1122486E-9	21.421053	28
CGCTCTC	90	9.4416464E-8	20.555555	29
GAAGTGT	45	0.0038217353	20.555555	6
CGAACTA	100	1.2838427E-8	20.35	29
CCGCTCT	95	1.6696504E-7	19.473684	28
GAGTCGC	50	0.007027465	18.5	22
ATACTGA	80	1.612602E-5	18.5	6
AGAGTGT	50	0.007027465	18.5	6
GTGTCAA	50	0.007027465	18.5	28
GCCCCGT	125	8.53106E-9	17.76	14
>>END_MODULE