FastQCFastQC Report
Fri 10 Feb 2017
ERR1632779.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632779.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252202
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9520.3774751984520345No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6360.2521788090498886No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3680.14591478259490406No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACGT250.005488312329.634
TTAACGG250.005488312329.635
TACGTGA250.005488312329.636
ATCAGAC358.850131E-426.4285723
GGTATCA2300.024.934781
TCGCCTA400.001927032123.12500235
ATATACT400.001927032123.1250024
GGTGTTC502.6940063E-422.237
GATTATC502.6940063E-422.224
GTATCAA5500.021.190911
GGTAAAC450.003817656820.55555535
TATCTAT450.003817656820.5555557
TCTAACC450.003817656820.5555553
TCGTCTT450.003817656820.55555514
CGATAGA450.003817656820.55555537
ACCGGGG555.127809E-420.18181834
AAGGTTC656.8748675E-519.9230757
AGTCCTA500.007020036718.531
CGAACTA609.2095917E-418.529
CCGGGGG609.2095917E-418.535
Produced by FastQC (version 0.11.5)