FastQCFastQC Report
Fri 10 Feb 2017
ERR1632778.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632778.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360875
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13730.38046414963630065No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9210.2552130239002425No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5090.14104606858330448No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC4810.13328714928992033No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT4530.1255282299965362No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC4410.12220297887080014No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA4340.12026324904745411No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4230.11721510218219604No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG3990.11056459993072393No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG3870.10723934880498788No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAAGG250.005491410429.59999821
GGTATCA3200.028.9062521
TGCTCGC556.241753E-726.9090910
GTATCAA8700.024.0287361
GTCAACC502.6971276E-422.19999915
TAGGACC502.6971276E-422.1999994
GCCGGCA555.133701E-420.18181815
GTCGCCA801.611667E-518.512
CGGCCTT1103.8298822E-818.524
TAGGAGT701.2162579E-418.54
GTTAGTA500.00702576218.49999827
TCTCGTC500.00702576218.49999812
AACGAAC500.00702576218.49999836
ACGAACC500.00702576218.49999837
ACGTGTC1156.3688276E-817.6956528
CCCGCCC1156.3688276E-817.69565214
CCGTATT953.592535E-617.5263161
ACGTGGG852.7147407E-517.41176636
ACGGGGG1603.274181E-1117.3437516
CTCGTCT752.0623185E-417.26666613
Produced by FastQC (version 0.11.5)