##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632778.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	360875
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.71560512642882	33.0	33.0	33.0	27.0	33.0
2	31.246326290266712	33.0	33.0	33.0	27.0	33.0
3	31.68883962590925	33.0	33.0	33.0	27.0	33.0
4	31.828292344994804	33.0	33.0	33.0	27.0	33.0
5	31.92943262902667	33.0	33.0	33.0	33.0	33.0
6	34.80384897817804	37.0	37.0	37.0	27.0	37.0
7	34.98669622445445	37.0	37.0	37.0	33.0	37.0
8	35.14964738482854	37.0	37.0	37.0	33.0	37.0
9	35.25838032559751	37.0	37.0	37.0	33.0	37.0
10	35.2753612746796	37.0	37.0	37.0	33.0	37.0
11	35.297202632490475	37.0	37.0	37.0	33.0	37.0
12	35.26015102182196	37.0	37.0	37.0	33.0	37.0
13	35.287110495323866	37.0	37.0	37.0	33.0	37.0
14	35.26354832005542	37.0	37.0	37.0	33.0	37.0
15	35.27431382057499	37.0	37.0	37.0	33.0	37.0
16	35.29371111880845	37.0	37.0	37.0	33.0	37.0
17	35.24332525112574	37.0	37.0	37.0	33.0	37.0
18	35.24850987183928	37.0	37.0	37.0	33.0	37.0
19	35.235341877381366	37.0	37.0	37.0	33.0	37.0
20	35.2543761690336	37.0	37.0	37.0	33.0	37.0
21	35.249191548320056	37.0	37.0	37.0	33.0	37.0
22	35.27576030481469	37.0	37.0	37.0	33.0	37.0
23	35.24372982334604	37.0	37.0	37.0	33.0	37.0
24	35.24702736404572	37.0	37.0	37.0	33.0	37.0
25	35.23526428818843	37.0	37.0	37.0	33.0	37.0
26	35.19521163837894	37.0	37.0	37.0	33.0	37.0
27	35.130358157256666	37.0	37.0	37.0	33.0	37.0
28	35.11383443020436	37.0	37.0	37.0	33.0	37.0
29	35.1623803255975	37.0	37.0	37.0	33.0	37.0
30	35.11112712157949	37.0	37.0	37.0	33.0	37.0
31	35.12442535503983	37.0	37.0	37.0	33.0	37.0
32	35.139045375822654	37.0	37.0	37.0	33.0	37.0
33	35.11861724974022	37.0	37.0	37.0	33.0	37.0
34	35.06401108417042	37.0	37.0	37.0	33.0	37.0
35	35.05944440595774	37.0	37.0	37.0	27.0	37.0
36	35.028494631104955	37.0	37.0	37.0	27.0	37.0
37	35.06126498094908	37.0	37.0	37.0	27.0	37.0
38	34.906599237963285	37.0	37.0	37.0	27.0	37.0
39	34.96767578801524	37.0	37.0	37.0	27.0	37.0
40	34.99966470384482	37.0	37.0	37.0	27.0	37.0
41	35.0371014894354	37.0	37.0	37.0	27.0	37.0
42	34.945659854520265	37.0	37.0	37.0	27.0	37.0
43	34.7155358503637	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	4.0
16	19.0
17	15.0
18	12.0
19	13.0
20	23.0
21	73.0
22	204.0
23	456.0
24	982.0
25	1850.0
26	2980.0
27	4403.0
28	6323.0
29	8576.0
30	10767.0
31	14119.0
32	17893.0
33	23553.0
34	33445.0
35	60276.0
36	174887.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.48333910633876	20.22777970211292	13.75988915829581	24.52899203325251
2	15.73896778662972	23.56937997921718	38.53439556633183	22.15725666782127
3	17.957464496016627	27.035122965015585	31.104399030135088	23.9030135088327
4	12.876757880152406	18.16390717007274	38.944232767578804	30.015102182196053
5	13.886248701073779	37.219812954624174	35.16203671631452	13.731901627987531
6	28.937720817457567	39.72622099064773	17.805334257014202	13.530723934880498
7	25.893730516106682	32.77561482507794	23.218565985452027	18.112088673363353
8	23.877242812608245	36.14963630065812	20.943817111188086	19.02930377554555
9	25.845237270523036	15.787183927952892	20.04599930723935	38.32157949428473
10	14.891305853827502	28.379632836854867	33.73910633875996	22.989954970557672
11	33.32095600969865	24.240249393834432	24.18344302043644	18.25535157603048
12	22.61447869760998	25.798960859023207	30.32906130931763	21.257499134049183
13	27.996674748874263	22.35677173536543	26.717007273986837	22.92954624177347
14	22.316037409075165	21.487772774506407	27.776931070315207	28.41925874610322
15	24.070107377900936	28.36937997921718	24.982611707655007	22.57790093522688
16	22.75413924489089	28.73737443713197	25.050779355732594	23.457706962244547
17	22.628056806373397	27.751714582611708	26.025355039833737	23.594873571181157
18	22.78766886040873	27.29726359542778	27.665812261863525	22.249255282299966
19	25.122549359196398	26.4415656390717	27.55164530654659	20.884239695185315
20	24.96238309664011	25.77041912019397	27.66387253204018	21.603325251125735
21	23.04731555247662	27.290613093176308	26.95088326983027	22.711188084516802
22	22.426047800484934	27.245445098718392	27.04371319709041	23.28479390370627
23	22.599237963283684	27.3360581918947	27.571873917561483	22.492829927260132
24	23.366262556286806	27.166193280221684	26.782680983720127	22.68486317977139
25	23.469622445445097	26.795150675441636	27.679667474887427	22.055559404225843
26	23.532802216834085	26.91541392448909	27.010460685833042	22.541323172843782
27	23.281191548320056	26.798198822306894	26.939244890890198	22.981364738482853
28	22.400554208520955	27.26124004156564	27.59404225839972	22.74416349151368
29	23.49844128853481	27.211361274679597	26.526913751298924	22.763283685486666
30	22.764946311049535	27.52892275718739	27.355732594388638	22.350398337374436
31	23.392033252511258	26.893522687911325	27.21579494284725	22.49864911673017
32	22.031728437824732	27.053134741946653	27.48486317977139	23.430273640457223
33	22.37506061655698	26.778247315552477	27.98060270176654	22.866089366124005
34	22.192171804641497	26.81676480775892	27.48015240734326	23.510910980256323
35	22.71811569102875	27.373190162798757	27.24766193280222	22.66103221337028
36	23.031243505368895	27.227156217526844	27.02930377554555	22.71229650155871
37	23.75005195704884	26.38586768271562	26.854728091444407	23.009352268791133
38	23.390647731208865	27.063387599584342	26.87689643228265	22.669068236924144
39	22.98053342570142	26.656875649463114	27.431104953238655	22.931485971596814
40	22.837824731555248	27.14291652234153	27.361274679598196	22.657984066505023
41	22.66795981988223	26.617249740214753	27.80658122618635	22.90820921371666
42	22.867474887426393	26.919293384135777	27.17118115691029	23.042050571527536
43	22.80706615864219	26.467059231035677	27.360166262556284	23.365708347765846
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7.0
1	10.5
2	14.0
3	73.5
4	133.0
5	133.0
6	171.0
7	209.0
8	218.5
9	228.0
10	287.0
11	346.0
12	346.0
13	542.5
14	739.0
15	1346.0
16	1953.0
17	2254.5
18	2556.0
19	2556.0
20	2465.5
21	2375.0
22	2854.5
23	3334.0
24	4237.0
25	5140.0
26	5140.0
27	6382.5
28	7625.0
29	10054.0
30	12483.0
31	13642.0
32	14801.0
33	14801.0
34	16398.5
35	17996.0
36	19560.0
37	21124.0
38	22862.0
39	24600.0
40	24600.0
41	26093.0
42	27586.0
43	26841.5
44	26097.0
45	26705.0
46	27313.0
47	27313.0
48	27342.5
49	27372.0
50	27784.5
51	28197.0
52	28823.0
53	29449.0
54	29449.0
55	25662.5
56	21876.0
57	19637.5
58	17399.0
59	15107.5
60	12816.0
61	12816.0
62	11221.5
63	9627.0
64	7868.5
65	6110.0
66	5148.0
67	4186.0
68	4186.0
69	3420.0
70	2654.0
71	2295.0
72	1936.0
73	1582.0
74	1228.0
75	1228.0
76	915.5
77	603.0
78	457.5
79	312.0
80	249.5
81	187.0
82	187.0
83	144.5
84	102.0
85	92.5
86	83.0
87	64.0
88	45.0
89	45.0
90	35.0
91	25.0
92	16.0
93	7.0
94	4.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	360875.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.59662958009599
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.37239586322637	53.26293570946405
2	5.600126052753977	6.674972759356478
3	1.5173912684925595	2.7129421606926916
4	0.7639512416186166	1.8211567665599642
5	0.4594649775137161	1.3691282034956036
6	0.3385240865639915	1.2104936754536733
7	0.23407383821161387	0.9765008281202211
8	0.16340730381540036	0.7790819644934898
9	0.1475365296072698	0.791341191408359
>10	1.1577013904678202	14.212482158199952
>50	0.15877844423671364	6.689458700031739
>100	0.08524388451646339	8.717091831557982
>500	9.367459836974E-4	0.39916235931755456
>1k	4.683729918487E-4	0.3832516918482535
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1373	0.38046414963630065	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	921	0.2552130239002425	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	509	0.14104606858330448	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	481	0.13328714928992033	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	453	0.1255282299965362	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	441	0.12220297887080014	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	434	0.12026324904745411	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	423	0.11721510218219604	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	399	0.11056459993072393	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	387	0.10723934880498788	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.7710426047800486E-4	2.7710426047800486E-4	0.0
2	0.0	0.0	2.7710426047800486E-4	2.7710426047800486E-4	0.0
3	0.0	0.0	5.542085209560097E-4	2.7710426047800486E-4	0.0
4	0.0	0.0	8.313127814340146E-4	2.7710426047800486E-4	0.0
5	2.7710426047800486E-4	0.0	8.313127814340146E-4	2.7710426047800486E-4	0.0
6	2.7710426047800486E-4	0.0	0.0013855213023900243	2.7710426047800486E-4	0.0
7	2.7710426047800486E-4	0.0	0.0030481468652580534	2.7710426047800486E-4	0.0
8	2.7710426047800486E-4	0.0	0.0033252511257360583	2.7710426047800486E-4	0.0
9	2.7710426047800486E-4	0.0	0.004433668167648078	0.0011084170419120194	0.0
10	2.7710426047800486E-4	0.0	0.005264980949082092	0.0011084170419120194	0.0
11	2.7710426047800486E-4	0.0	0.00914444059577416	0.0013855213023900243	0.0
12	2.7710426047800486E-4	0.0	0.009421544856252165	0.0013855213023900243	0.0
13	2.7710426047800486E-4	0.0	0.009698649116730169	0.0013855213023900243	0.0
14	2.7710426047800486E-4	0.0	0.011084170419120194	0.0013855213023900243	0.0
15	2.7710426047800486E-4	0.0	0.013301004502944233	0.0013855213023900243	0.0
16	2.7710426047800486E-4	0.0	0.014409421544856253	0.0013855213023900243	0.0
17	2.7710426047800486E-4	0.0	0.014409421544856253	0.0013855213023900243	0.0
18	2.7710426047800486E-4	0.0	0.014963630065812262	0.0016626255628680291	0.0
19	2.7710426047800486E-4	0.0	0.015517838586768272	0.0016626255628680291	0.0
20	2.7710426047800486E-4	0.0	0.015517838586768272	0.0016626255628680291	0.0
21	2.7710426047800486E-4	0.0	0.01662625562868029	0.0016626255628680291	0.0
22	2.7710426047800486E-4	0.0	0.016903359889158295	0.001939729823346034	0.0
23	2.7710426047800486E-4	0.0	0.016903359889158295	0.002216834083824039	0.0
24	2.7710426047800486E-4	0.0	0.016903359889158295	0.0027710426047800486	0.0
25	2.7710426047800486E-4	0.0	0.0171804641496363	0.0027710426047800486	0.0
26	2.7710426047800486E-4	0.0	0.0171804641496363	0.0027710426047800486	0.0
27	2.7710426047800486E-4	0.0	0.0171804641496363	0.0033252511257360583	0.0
28	2.7710426047800486E-4	0.0	0.0171804641496363	0.006927606511950121	0.0
29	2.7710426047800486E-4	0.0	0.0171804641496363	0.023553862140630412	0.0
30	2.7710426047800486E-4	0.0	0.0171804641496363	0.048770349844128855	0.0
31	2.7710426047800486E-4	0.0	0.0171804641496363	0.11028749567024593	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGAAGG	25	0.0054914104	29.599998	21
GGTATCA	320	0.0	28.906252	1
TGCTCGC	55	6.241753E-7	26.90909	10
GTATCAA	870	0.0	24.028736	1
GTCAACC	50	2.6971276E-4	22.199999	15
TAGGACC	50	2.6971276E-4	22.199999	4
GCCGGCA	55	5.133701E-4	20.181818	15
GTCGCCA	80	1.611667E-5	18.5	12
CGGCCTT	110	3.8298822E-8	18.5	24
TAGGAGT	70	1.2162579E-4	18.5	4
GTTAGTA	50	0.007025762	18.499998	27
TCTCGTC	50	0.007025762	18.499998	12
AACGAAC	50	0.007025762	18.499998	36
ACGAACC	50	0.007025762	18.499998	37
ACGTGTC	115	6.3688276E-8	17.695652	8
CCCGCCC	115	6.3688276E-8	17.695652	14
CCGTATT	95	3.592535E-6	17.526316	1
ACGTGGG	85	2.7147407E-5	17.411766	36
ACGGGGG	160	3.274181E-11	17.34375	16
CTCGTCT	75	2.0623185E-4	17.266666	13
>>END_MODULE