FastQCFastQC Report
Fri 10 Feb 2017
ERR1632777.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632777.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430613
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT35820.8318374038870169No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26410.6133117207330016No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14240.33069136324263315No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC12390.2877293532708023No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC11910.2765824533862192No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA11660.2707767763629988No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG11580.2689189597155683No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC11160.25916542231655804No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG11080.2573076056691275No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10900.2531275182124088No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG10430.24221284540875448No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT10230.23756830379017818No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA10010.23245930800974424No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT9330.2166678665065848No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT9310.21620341234472717No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG9310.21620341234472717No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC8910.20691432910757454No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG8740.20296646873178467No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG8620.2001797437606389No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT8390.19483852089927614No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC7740.17974376063890316No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA7670.17811817107240144No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7500.17417031069661157No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA7330.1702224503208217No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG7170.16650681702596065No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC7130.1655779087022454No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG7110.16511345454038778No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC7030.16325563789295724No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6940.16116559416459791No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC6790.15768218795066566No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC6700.15559214422230633No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT6530.15164428384651646No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC6510.15117982968465885No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG6430.14932201303722833No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG6430.14932201303722833No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT6310.14653528806608254No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG6280.14583860682329608No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG6230.144677471418652No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT6210.1442130172567944No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC6210.1442130172567944No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA6170.14328410893307914No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG5990.13910402147636045No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG5920.13747843190985873No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC5910.13724620482892994No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA5880.13654952358614347No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT5690.13213720904849596No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA5630.1307438465629231No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5580.12958271115827902No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA5570.1293504840773502No Hit
TATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCC5470.12702821326806205No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA5450.1265637591062044No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT5410.12563485078248915No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT5400.12540262370156036No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG5290.12284812581134337No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA5130.11913249251648231No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5100.11843581127369589No Hit
CCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTAT5090.11820358419276705No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT5060.11750690294998062No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC5050.11727467586905178No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA5030.11681022170719417No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGC4960.11518463214069245No Hit
GCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGT4930.114487950897906No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA4900.11379126965511957No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT4880.11332681549326193No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG4870.11309458841233311No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT4840.11239790716954666No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC4840.11239790716954666No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4830.11216568008861785No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT4790.11123677176490258No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG4790.11123677176490258No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG4660.10821781971282798No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT4630.10752113847004154No Hit
TTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAG4500.10450218641796694No Hit
CAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGG4490.10426995933703813No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC4450.10334105101332286No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT4440.10310882393239405No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA4350.10101878020403472No Hit
GATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTA4330.10055432604217708No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4310.10008987188031945No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGAA200.001840658137.02
GGTATCA4950.029.8989871
TAGATCT250.00549257329.64
AGGGACG250.00549257329.634
CGACAAG358.860352E-426.42857234
CCGACAA358.860352E-426.42857233
GTATCAA17850.026.324931
CATGTTT2200.026.06818230
CGGCGTC509.073856E-625.90000224
GCCTCTG509.073856E-625.90000220
CACATGT2350.025.1914928
AGTGACA2350.025.1914923
ACATGTT2300.024.93478429
CAAAGTA601.3341632E-624.66666830
TCAAAGT601.3341632E-624.66666829
TGTTTAC751.3686076E-824.66666614
GACTGCC451.3213356E-424.66666626
GTGACAC2450.024.16326724
ATGTTTA2350.023.61702231
GACACAT2550.023.21568726
Produced by FastQC (version 0.11.5)