FastQCFastQC Report
Fri 10 Feb 2017
ERR1632776.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632776.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419467
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13650.3254129645478667No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9050.21574998748411675No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT5360.12778120805689125No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5070.12086767254635049No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC5010.11943728588899724No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG4810.11466933036448637No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4490.10704060152526898No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4340.10346463488188583No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC4290.10227264600075811No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA4250.10131905489585594No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGT200.001840603937.025
CGTCCGC200.001840603937.029
AGTCGCC200.001840603937.023
CCGTCCG250.00549241329.628
ACCGTTC250.00549241329.68
CGCCGTC250.00549241329.626
GGTATCA3300.029.1515141
CGAGTCG358.859967E-426.42857221
TTAGACT400.001929154223.1254
TATTCGA400.001929154223.12515
GTATCAA9050.022.4861891
TTAACGG603.7190286E-521.58333235
ATTCGAG450.003821818420.55555716
TTACATA450.003821818420.5555574
TACACCC555.135614E-420.1818185
TAAACCG500.00702761618.55
CCTACAC1002.8641261E-718.53
GTTAGTA801.6126853E-518.527
TTATCGA500.00702761618.531
CGTTAAA500.00702761618.517