##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632776.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	419467
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.60806928792968	33.0	33.0	33.0	27.0	33.0
2	31.141739397854895	33.0	33.0	33.0	27.0	33.0
3	31.601868561770054	33.0	33.0	33.0	27.0	33.0
4	31.74421825793209	33.0	33.0	33.0	27.0	33.0
5	31.84593305313648	33.0	33.0	33.0	33.0	33.0
6	34.70487308894383	37.0	37.0	37.0	27.0	37.0
7	34.89050628535737	37.0	37.0	37.0	27.0	37.0
8	35.06393589960593	37.0	37.0	37.0	33.0	37.0
9	35.15785508752774	37.0	37.0	37.0	33.0	37.0
10	35.17385873024576	37.0	37.0	37.0	33.0	37.0
11	35.20613540516894	37.0	37.0	37.0	33.0	37.0
12	35.164196468375344	37.0	37.0	37.0	33.0	37.0
13	35.2073822255386	37.0	37.0	37.0	33.0	37.0
14	35.168563915635794	37.0	37.0	37.0	33.0	37.0
15	35.19339304402968	37.0	37.0	37.0	33.0	37.0
16	35.194499209711374	37.0	37.0	37.0	33.0	37.0
17	35.16235842152064	37.0	37.0	37.0	33.0	37.0
18	35.158715703499915	37.0	37.0	37.0	33.0	37.0
19	35.17569200914494	37.0	37.0	37.0	33.0	37.0
20	35.173334255138066	37.0	37.0	37.0	33.0	37.0
21	35.1786314537258	37.0	37.0	37.0	33.0	37.0
22	35.187962342687264	37.0	37.0	37.0	33.0	37.0
23	35.1747408020178	37.0	37.0	37.0	33.0	37.0
24	35.148860339430755	37.0	37.0	37.0	33.0	37.0
25	35.15985524487028	37.0	37.0	37.0	33.0	37.0
26	35.113756266881545	37.0	37.0	37.0	33.0	37.0
27	35.04435629024452	37.0	37.0	37.0	27.0	37.0
28	35.027997434839925	37.0	37.0	37.0	27.0	37.0
29	35.07212486321927	37.0	37.0	37.0	33.0	37.0
30	35.03392161957913	37.0	37.0	37.0	27.0	37.0
31	35.01736489402027	37.0	37.0	37.0	27.0	37.0
32	35.07125471133605	37.0	37.0	37.0	33.0	37.0
33	35.042506323501016	37.0	37.0	37.0	27.0	37.0
34	34.98056104532657	37.0	37.0	37.0	27.0	37.0
35	34.97334474464022	37.0	37.0	37.0	27.0	37.0
36	34.963486996593296	37.0	37.0	37.0	27.0	37.0
37	34.98230611704854	37.0	37.0	37.0	27.0	37.0
38	34.8328259433996	37.0	37.0	37.0	27.0	37.0
39	34.91299434758873	37.0	37.0	37.0	27.0	37.0
40	34.947695527896116	37.0	37.0	37.0	27.0	37.0
41	34.960454576879705	37.0	37.0	37.0	27.0	37.0
42	34.89003187378268	37.0	37.0	37.0	27.0	37.0
43	34.662674298574146	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	11.0
16	21.0
17	14.0
18	14.0
19	18.0
20	35.0
21	79.0
22	267.0
23	624.0
24	1258.0
25	2259.0
26	3650.0
27	5511.0
28	7729.0
29	10478.0
30	13659.0
31	16994.0
32	21809.0
33	28026.0
34	39622.0
35	70837.0
36	196549.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.41350809479649	20.0399554672954	13.69142268640918	24.855113751498926
2	15.994345204747932	23.271198926256414	38.164623200394786	22.569832668600867
3	18.056724366875105	26.797578832184655	30.707540760059793	24.43815604088045
4	12.862752016249193	18.19189590599499	38.808773991756205	30.136578085999616
5	13.830170192172448	37.11996414497445	35.360350158653716	13.689515504199376
6	29.35797094884699	39.54661510917426	17.55680423013014	13.538609711848606
7	26.17297665847373	32.50267601503813	23.44594449622974	17.8784028302584
8	24.091764072024734	36.11011116488305	20.662412061020294	19.135712702071917
9	25.917891037912398	15.76905930621479	19.848283655210064	38.46476600066275
10	15.15017867913328	28.14762543894991	33.77381295787273	22.928382924044087
11	33.66200440082295	23.92107126424725	24.129192522892147	18.28773181203766
12	22.706911389930553	25.916699049031273	29.89078997871108	21.485599582327097
13	28.196735380852367	22.077779658471346	26.488376916420126	23.237108044256164
14	22.217719153115738	21.23504351951405	27.324437917643102	29.222799409727106
15	24.10487594971714	28.53430663198774	24.623152715231473	22.73766470306365
16	22.97248651264581	28.519525969861753	24.777634474225625	23.730353043266813
17	22.662807801328828	27.546147849532858	25.914791866821467	23.876252482316847
18	23.033754741135777	27.12036942119404	27.343509739741144	22.502366097929038
19	25.106861803193098	26.302188253187975	27.374501450650467	21.216448492968457
20	24.875377562478096	25.7424302746104	27.35256885523772	22.029623307673788
21	22.9789232526039	27.344463330846047	26.68076392183414	22.995849494715912
22	22.76221967401488	27.017143184088376	26.775646236771905	23.444990905124836
23	22.895722428701184	27.193080742942833	27.325629906524227	22.585566921831752
24	23.465493113880235	27.22502604495705	26.18942610503329	23.120054736129422
25	23.620928463979286	26.733926625932437	27.306558084426186	22.33858682566209
26	23.56752736210477	26.87791888277266	26.69578298173635	22.858770773386226
27	23.2216121888015	26.851695127387853	26.636421935456184	23.29027074835446
28	22.61822741717465	27.02715589068985	27.32515311097178	23.029463581163714
29	23.713188403378574	27.285102284565887	26.406844876950984	22.59486443510455
30	23.13555059158408	27.122276603403844	27.10654235017296	22.635630454839117
31	23.70961243673519	26.682909501820166	26.907241809248404	22.70023625219624
32	22.46231527152315	26.620926080001528	27.30155173112545	23.615206917349877
33	22.541463333230027	26.657162541987812	27.675359444247107	23.12601468053506
34	22.678065259007262	26.60686061120422	27.212152565040874	23.502921564747645
35	23.118147553919616	27.127998150033257	26.898421091528057	22.85543320451907
36	23.395880963222375	26.890077169360165	26.730589057065274	22.983452810352183
37	23.850505522484486	26.127204285438427	26.661215304183646	23.361074887893444
38	23.70055332123862	26.783513363387346	26.596371109050292	22.91956220632374
39	23.018258885681114	26.518653434000765	27.303458913335255	23.159628766982863
40	23.137219376017658	26.72915867040792	27.21835090722274	22.91527104635168
41	22.852334033428136	26.269527757845072	27.549247020623792	23.328891188103
42	23.064269656492645	26.743939332533905	27.0705442859629	23.12124672501055
43	23.17250224689904	26.19371726500535	27.126567763375903	23.507212724719704
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	8.5
2	11.0
3	64.0
4	117.0
5	117.0
6	163.5
7	210.0
8	188.0
9	166.0
10	249.0
11	332.0
12	332.0
13	498.5
14	665.0
15	1252.0
16	1839.0
17	2150.5
18	2462.0
19	2462.0
20	2389.0
21	2316.0
22	2736.5
23	3157.0
24	4031.0
25	4905.0
26	4905.0
27	6258.0
28	7611.0
29	10151.5
30	12692.0
31	14128.0
32	15564.0
33	15564.0
34	17925.5
35	20287.0
36	22237.5
37	24188.0
38	26829.0
39	29470.0
40	29470.0
41	31798.5
42	34127.0
43	33349.0
44	32571.0
45	33142.5
46	33714.0
47	33714.0
48	33803.0
49	33892.0
50	33778.5
51	33665.0
52	33690.0
53	33715.0
54	33715.0
55	29553.0
56	25391.0
57	22422.5
58	19454.0
59	17139.5
60	14825.0
61	14825.0
62	13101.0
63	11377.0
64	9200.5
65	7024.0
66	6072.0
67	5120.0
68	5120.0
69	4195.0
70	3270.0
71	2717.0
72	2164.0
73	1783.5
74	1403.0
75	1403.0
76	1082.5
77	762.0
78	590.5
79	419.0
80	325.0
81	231.0
82	231.0
83	183.5
84	136.0
85	137.0
86	138.0
87	95.5
88	53.0
89	53.0
90	33.5
91	14.0
92	9.0
93	4.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	419467.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.905018425817765
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.70195479385053	49.0297939873045
2	6.193423009764576	6.9248685495954465
3	1.780046716477944	2.985406334505476
4	0.9384805521648933	2.0986309024419993
5	0.5714798085568943	1.597429461367799
6	0.3879787382230636	1.3013975105511537
7	0.30431339366637555	1.1908852116099302
8	0.24228643876603798	1.0836022258832896
9	0.17143008019041547	0.8625421612606133
>10	1.4346744122787296	16.20891222218097
>50	0.17357644432778577	6.544402624827973
>100	0.0982020578328943	9.253759787864729
>500	0.0017228431198753385	0.5896921162728194
>1k	4.3071077996883464E-4	0.32867690433335994
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1365	0.3254129645478667	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	905	0.21574998748411675	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	536	0.12778120805689125	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	507	0.12086767254635049	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	501	0.11943728588899724	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	481	0.11466933036448637	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	449	0.10704060152526898	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	434	0.10346463488188583	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	429	0.10227264600075811	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	425	0.10131905489585594	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	4.7679555245108674E-4	0.0	0.0
2	2.3839777622554337E-4	0.0	4.7679555245108674E-4	0.0	0.0
3	2.3839777622554337E-4	0.0	7.151933286766301E-4	0.0	0.0
4	2.3839777622554337E-4	0.0	9.535911049021735E-4	0.0	0.0
5	2.3839777622554337E-4	0.0	0.0011919888811277167	0.0	0.0
6	2.3839777622554337E-4	0.0	0.0014303866573532602	0.0	0.0
7	2.3839777622554337E-4	0.0	0.002622375538480977	0.0	0.0
8	2.3839777622554337E-4	0.0	0.004052762195834237	0.0	0.0
9	2.3839777622554337E-4	0.0	0.004529557748285324	2.3839777622554337E-4	0.0
10	0.0011919888811277167	0.0	0.005244751076961954	2.3839777622554337E-4	0.0
11	0.0011919888811277167	0.0	0.008582319944119562	4.7679555245108674E-4	0.0
12	0.0011919888811277167	0.0	0.010012706601472821	4.7679555245108674E-4	0.0
13	0.0011919888811277167	0.0	0.010727899930149451	4.7679555245108674E-4	0.0
14	0.0011919888811277167	0.0	0.010966297706374995	4.7679555245108674E-4	0.0
15	0.0011919888811277167	0.0	0.0126350821399538	4.7679555245108674E-4	0.0
16	0.0011919888811277167	0.0	0.013588673244855971	4.7679555245108674E-4	0.0
17	0.0011919888811277167	0.0	0.013827071021081515	4.7679555245108674E-4	0.0
18	0.0011919888811277167	0.0	0.014065468797307058	7.151933286766301E-4	0.0
19	0.0011919888811277167	0.0	0.014780662125983689	7.151933286766301E-4	0.0
20	0.0011919888811277167	0.0	0.014780662125983689	7.151933286766301E-4	0.0
21	0.0011919888811277167	0.0	0.014780662125983689	0.0011919888811277167	0.0
22	0.0011919888811277167	0.0	0.015257457678434776	0.0014303866573532602	0.0
23	0.0011919888811277167	0.0	0.01549585545466032	0.001907182209804347	0.0
24	0.0011919888811277167	0.0	0.015734253230885863	0.0021455799860298904	0.0
25	0.0011919888811277167	0.0	0.015734253230885863	0.0021455799860298904	0.0
26	0.0011919888811277167	0.0	0.015972651007111406	0.0021455799860298904	0.0
27	0.0011919888811277167	0.0	0.015972651007111406	0.003337568867157607	0.0
28	0.0011919888811277167	0.0	0.015972651007111406	0.008343922167894018	0.0
29	0.0011919888811277167	0.0	0.015972651007111406	0.021694197636524446	0.0
30	0.0011919888811277167	0.0	0.015972651007111406	0.04338839527304889	0.0
31	0.0011919888811277167	0.0	0.015972651007111406	0.11776850145541842	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCGT	20	0.0018406039	37.0	25
CGTCCGC	20	0.0018406039	37.0	29
AGTCGCC	20	0.0018406039	37.0	23
CCGTCCG	25	0.005492413	29.6	28
ACCGTTC	25	0.005492413	29.6	8
CGCCGTC	25	0.005492413	29.6	26
GGTATCA	330	0.0	29.151514	1
CGAGTCG	35	8.859967E-4	26.428572	21
TTAGACT	40	0.0019291542	23.125	4
TATTCGA	40	0.0019291542	23.125	15
GTATCAA	905	0.0	22.486189	1
TTAACGG	60	3.7190286E-5	21.583332	35
ATTCGAG	45	0.0038218184	20.555557	16
TTACATA	45	0.0038218184	20.555557	4
TACACCC	55	5.135614E-4	20.181818	5
TAAACCG	50	0.007027616	18.5	5
CCTACAC	100	2.8641261E-7	18.5	3
GTTAGTA	80	1.6126853E-5	18.5	27
TTATCGA	50	0.007027616	18.5	31
CGTTAAA	50	0.007027616	18.5	17
>>END_MODULE