FastQCFastQC Report
Fri 10 Feb 2017
ERR1632775.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632775.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452372
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT72371.5997895537301159No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT56071.2394666336554871No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT28320.6260334415038951No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG21880.48367272952348955No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA21330.4715145941835481No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA19250.42553473689795124No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA15190.33578559238856515No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15070.33313290831439607No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT14450.31942737393118936No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG13880.3068271245788864No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC12680.2803002838371959No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT12020.26571052142926616No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC11750.2597419822623858No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC10860.24006790871229874No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG10840.23962579469993722No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA10540.23299408451451462No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA10350.22879400139708028No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT10120.22370969025492293No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA9640.21309895395824674No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC9510.21022521287789694No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG9460.20911992784699318No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA9370.2071304147913664No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC9190.20315138868011282No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG9110.20138293263066678No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT8950.19784602053177472No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT8810.19475122244524418No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC8790.19430910843288268No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT8740.19320382340197892No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA8320.18391942914238724No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT8200.1812667450682182No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC8090.1788351180002299No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA8030.17750877596314538No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG7690.16999283775299973No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC7640.16888755272209596No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC7460.1649085266108424No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT7290.1611505575057696No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7260.16048738648722732No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC7100.15695047438833526No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG7050.1558451893574315No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA6990.15451884732034699No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC6730.14877136515964737No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA6580.14545551006693605No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA6560.14501339605457456No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG6320.13970802790623646No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA6270.1386027428753327No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT6080.13440265975789836No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG5990.13241314670227158No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT5970.13197103268991006No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG5930.13108680466518705No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG5810.12843412059101803No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG5690.12578143651684898No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG5680.12556037951066823No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5610.12401298046740294No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG5500.12158135339941464No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG5480.12113923938705314No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC5460.12069712537469163No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC5390.11914972633142636No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA5370.11870761231906485No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT5300.11716021327579956No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG5210.11517070022017278No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC5060.11185484512746147No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA5060.11185484512746147No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT5030.11119167410891921No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG4970.10986533207183469No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA4920.10876004704093091No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4890.10809687602238865No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC4760.10522313494203885No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC4700.10389679290495432No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT4670.10323362188641207No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG4610.10190727984932754No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC4610.10190727984932754No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT4610.10190727984932754No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT4540.10035988080606226No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACTT200.00184075637.05
TGCGTCT303.5960582E-430.83333410
TTAGATC250.00549286429.63
GGTATCA12650.028.2252981
GTATTAT405.9322876E-527.7500021
GGGTATT405.9322876E-527.75000218
ACTAGTG905.456968E-1226.7222218
GATGGAC358.861048E-426.4285731
GCCGTCC2700.023.98148227
CTACCGT851.9681465E-923.9411778
CCGCTCT3050.023.65573928
CGCTCTC3050.023.65573929
GAGTCGC2900.023.60344922
GTATCAA34300.023.5699711
CGAGTCG3000.023.43333421
ACCGTTT952.8012437E-1023.36842210
GCGACGG400.001929386423.12500217
CTAGTGC802.7164788E-823.1250029
CGAGGGA400.001929386423.12500216
GATCTGC400.001929386423.1250026
Produced by FastQC (version 0.11.5)